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Genetica

, Volume 140, Issue 4–6, pp 115–123 | Cite as

Microsatellite genetic diversity and differentiation of native and introduced grass carp populations in three continents

  • Qin Chen
  • Chenghui WangEmail author
  • Guoqing Lu
  • Jinliang Zhao
  • Duane C. Chapman
  • Jeney Zsigmond
  • Sifa Li
Article

Abstract

Grass carp (Ctenopharyngodon idella), a freshwater species native to China, has been introduced to about 100 countries/regions and poses both biological and environmental challenges to the receiving ecosystems. In this study, we analyzed genetic variation in grass carp from three introduced river systems (Mississippi River Basin in US, Danube River in Hungary, and Tone River in Japan) as well as its native ranges (Yangtze, Pearl, and Amur Rivers) in China using 21 novel microsatellite loci. The allelic richness, observed heterozygosity, and within-population gene diversity were found to be lower in the introduced populations than in the native populations, presumably due to the small founder population size of the former. Significant genetic differentiation was found between all pairwise populations from different rivers. Both principal component analysis and Bayesian clustering analysis revealed obvious genetic distinction between the native and introduced populations. Interestingly, genetic bottlenecks were detected in the Hungarian and Japanese grass carp populations, but not in the North American population, suggesting that the Mississippi River Basin grass carp has experienced rapid population expansion with potential genetic diversification during the half-century since its introduction. Consequently, the combined forces of the founder effect, introduction history, and rapid population expansion help explaining the observed patterns of genetic diversity within and among both native and introduced populations of the grass carp.

Keywords

Ctenopharyngodon idella Microsatellite Genetic differentiation Bottleneck effect 

Notes

Acknowledgments

The authors thank Mr. Minghu Tang (Hangjiang Chinese Farmed Fish Farm, China), Lixia Fu (Yangzhou University, Jiangsu, China), Kevin Irons (Illinois Natural History Survey, USA), Mark Pegg (University of Nebraska, Lincoln, USA), Kirk Steffensen (Nebraska Game and Parks Commission), and James T. Lamer (Western Illinois University, USA). We also acknowledge Mary Christman (University of Nebraska at Omaha, USA) for proofreading this paper. This research was supported by the National Natural Science Foundation of China (Grant No. 30630051).

Supplementary material

10709_2012_9663_MOESM1_ESM.doc (56 kb)
Supplementary material 1 (DOC 56 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Qin Chen
    • 1
  • Chenghui Wang
    • 1
    Email author
  • Guoqing Lu
    • 2
  • Jinliang Zhao
    • 1
  • Duane C. Chapman
    • 3
  • Jeney Zsigmond
    • 4
  • Sifa Li
    • 1
  1. 1.Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of AgricultureShanghai Ocean UniversityShanghaiChina
  2. 2.Department of BiologyUniversity of Nebraska at OmahaOmahaUSA
  3. 3.USGS Columbia Environmental Research CenterColumbiaUSA
  4. 4.Research Institute for FisheriesAquaculture and IrrigationSzarvasHungary

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