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Genomic content and new insights on the origin of the B chromosome of the cichlid fish Astatotilapia latifasciata

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Abstract

B chromosomes are additional chromosomes widely studied in a diversity of eukaryotic groups, including fungi, plants and animals, but their origin, evolution and possible functions are not clearly understood. To further understand the genomic content and the evolutionary history of B chromosomes, classical and molecular cytogenetic analyses were conducted in the cichlid fish Astatotilapia latifasciata, which harbor 1–2 B chromosomes. Through cytogenetic mapping of several probes, including transposable elements, rRNA genes, a repeated DNA genomic fraction (C 0 t − 1 DNA), whole genome probes (comparative genomic hybridization), and BAC clones from Oreochromis niloticus, we found similarities between the B chromosome and the 1st chromosome pair and chromosomes harboring rRNA genes. Based on the cytogenetic mapping data, we suggest the B chromosome may have evolved from a small chromosomal fragment followed by the invasion of the proto-B chromosome by several repeated DNA families.

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Acknowledgments

We thank TD Kocher for providing the BAC clones and the critical review of the manuscript, and F Yang for helpful assistance with BACs hybridization. Financial support was provided by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), the Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) from Brazil.

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Correspondence to Cesar Martins.

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Fantinatti, B.E.A., Mazzuchelli, J., Valente, G.T. et al. Genomic content and new insights on the origin of the B chromosome of the cichlid fish Astatotilapia latifasciata . Genetica 139, 1273–1282 (2011). https://doi.org/10.1007/s10709-012-9629-x

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  • DOI: https://doi.org/10.1007/s10709-012-9629-x

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