Calreticulin and calnexin are Ca2+-binding chaperones localized in the endoplasmic reticulum of eukaryotes acting in glycoprotein folding quality control and Ca2+ homeostasis. The evolutionary histories of calreticulin and calnexin gene families were inferred by comprehensive phylogenetic analyses using 18 completed genomes and ESTs covering the major green plants groups, from green algae to angiosperms. Calreticulin and calnexin possibly share a common origin, and both proteins are present along all green plants lineages. The calreticulin founder gene within green plants duplicated in early tracheophytes leading to two possible groups of orthologs with specialized functions, followed by lineage-specific gene duplications in spermatophytes. Calnexin founder gene in land plants was inherited from basal green algae during evolution in a very conservative copy number. A comprehensive classification in possible groups of orthologs and a catalog of calreticulin and calnexin genes from green plants are provided.
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I would like to thank the JGI (www.jgi.doe.gov/) for providing most of the data used in this work.
Electronic supplementary material
Below is the link to the electronic supplementary material.
CRT and CNX Possible Groups of Orthologous (PoGOs) in green plants (XLS 44 kb)
Detailed phylogenetic analysis of CNXs in green plants. Tree topology is a consensus from NJ, MP and Bayesian analyses. Bootstrap values and posterior probabilities from the original trees higher than 50% are shown (NJ/MP/Bayesian).
Detailed phylogenetic analysis of CRTs in green plants. Tree topology is a consensus from NJ, MP and Bayesian analyses. Bootstrap values and posterior probabilities from the original trees higher than 50% are shown (NJ/MP/Bayesian).
Shared intron positions betweenArabidopsis thaliana,Sorghum bicolor, Physcomitrella patens patensandVolvox carteri CNX genes. Single amino acid positions, highlighted in blue, represents intron location within the corresponding codon and double amino acid positions, highlighted in red, represent intron location between two codons. Supplementary material 4 (TIFF 349 kb)
Shared intron positions betweenArabidopsis thaliana,Sorghum bicolor, Physcomitrella patens patensandVolvox carteri CRT genes. Single amino acid positions, highlighted in blue, represents intron location within the corresponding codon and double amino acid positions, highlighted in red, represent intron location between two codons. Supplementary material 5 (TIFF 341 kb)
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Del Bem, L.E.V. The evolutionary history of calreticulin and calnexin genes in green plants. Genetica 139, 255–259 (2011). https://doi.org/10.1007/s10709-010-9544-y
- Green plants