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Genetica

, Volume 138, Issue 7, pp 787–794 | Cite as

Chromosomal mapping of the major and minor ribosomal genes, (GATA)n and U2 snRNA gene by double-colour FISH in species of the Batrachoididae family

  • María Úbeda-Manzanaro
  • Manuel A. Merlo
  • José L. Palazón
  • Ismael Cross
  • Carmen Sarasquete
  • Laureana Rebordinos
Original Research

Abstract

In the present study dual-colour fluorescence in situ hybridization (FISH) was performed to study the chromosomal distribution of 18S and 5S rDNAs, (GATA)n and 5S rDNA, and U2 snRNA and 18S rDNA in four species of Batrachoididae family: Amphichthys cryptocentrus, Batrachoides manglae, Porichthys plectrodon and Thalassophryne maculosa. The 18S rDNA signals were present in only one pair of chromosomes in all the four Batrachoididae species. The 5S rDNA was mapped on one pair of chromosomes, except in B. manglae, which showed a hybridization signal in two pairs. The two ribosomal genes are located on different chromosome pairs, except in A. cryptocentrus, in which they appear co-located. In all the cases, the (GATA)n probe produced disperse hybridization signals in all four species. The U2 snRNA signals appear very widely scattered in A. cryptocentrus, P. plectrodon, but show a degree of clustering in a specific chromosome pair in B. manglae. In T. maculosa, they are thinly dispersed and strong hybridization signals are observed co-located to the 18S rDNA-bearing chromosomes. Finally, a double-colour FISH with U2 snRNA and 5S rDNA probes was performed in B. manglae, and this showed that these genes were not co-located. These results have been compared with those from another Batrachoididae species, and evolutive processes of these species are discussed.

Keywords

45S rDNA 5S rDNA Batrachoididae (GATA)n Toadfish U2 snRNA 

Notes

Acknowledgments

María Úbeda-Manzanaro holds a CSIC JAE-Pre. fellowship. This work forms part of the Proyecto de Excelencia/IP: Carmen Sarasquete (RNM-03074; Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía, Spain).

References

  1. Barzotti R, Pelliccia F, Rocchi A (2003) Identification and characterization of U1 small nuclear RNA genes from two crustacean isopod species. Chromosome Res 11:365–373CrossRefPubMedGoogle Scholar
  2. Boroń A, Ozouf-Coastaz C, Coutanceau J-P, Woroniecka K (2006) Gene mapping of 28S and 5S rDNA sites in the spined loach Cobitis taenia (Pisces, Cobitidae) from a diploid and a diploid-tetraploid population. Genetica 128:71–79CrossRefPubMedGoogle Scholar
  3. Brum MJI, Affonso PRAM, Moto LCG, Pauls E, Netto MRCB (2001) Cytogenetic characterization of Porichthys porisissimus (Valenciennes, 1857) (Batrachiodidae, Batrachoidiformes) from the Rio de Janeiro Coast, Brazil. Chromosome Sci 5:15–18Google Scholar
  4. Cross I (2005) Caracterización poblacional y citogenética de Crassostrea angulata. Análisis molecular del ADNr 5S en ostreidos. Tesis doctoral, Universidad de CádizGoogle Scholar
  5. Cross I, Vega L, Rebordinos L (2003) Nucleolar organizing regions in Crassostrea angulata: chromosomal location and polymorphism. Genetica 119:65–74CrossRefPubMedGoogle Scholar
  6. Cross I, Díaz E, Sánchez I, Rebordinos L (2005) Molecular and cytogenetic characterization of Crassostrea angulata chromosomes. Aquaculture 247:135–144CrossRefGoogle Scholar
  7. Cross I, Merlo A, Manchado M, Infante C, Cañavate JP, Rebordinos L (2006) Cytogenetic characterization of the Solea senegalensis (Teleostei: Pleurenectiformes. Soleidae): Ag-NOR, (GATA)n, (TTAGGG)n and ribosomal genes by one-color and two-color FISH. Genetica 128:253–259CrossRefPubMedGoogle Scholar
  8. Demas S, Duronslet M, Wachtel S, Caillouet C, Nakamura D (1990) Sex-specific DNA in reptiles with temperature sex determination. J Exp Zool 253:319–324CrossRefPubMedGoogle Scholar
  9. Diniz D, Moreira-Filho O, Bertollo LAC (2008) Molecular cytogenetics and characterization of a ZZ/ZW sex chromosome system in Triportheus nemaratus (Characiformes, Characidae). Genetica 133:85–91CrossRefPubMedGoogle Scholar
  10. Dover GA (1989) Linkage disequilibrium and molecular drive in the rDNA gene family. Genetics 122:249–252PubMedGoogle Scholar
  11. Epplen JT, McCarrey JR, Sutou S, Ohno S (1982) Base sequence of a cloned snake W-chromosome DNA fragment and identification of a male-specific putative mRNA in the mouse. Proc Natl Acad Sci USA 79:3798–3802CrossRefPubMedGoogle Scholar
  12. Epplen JT, Studer R, McLaren A (1988) Heterogeneity in the Sxr (sex-reversal) locus of the mouse as revealed by synthetic GATA-GACA probes. Genet Res 51:239–246CrossRefPubMedGoogle Scholar
  13. Fujiwara A, Fujiwara M, Nishida-Umehara C, Abe S, Masaoka T (2007) Characterization of Japanese flounder karyotype by chromosome bandings and fluorescence in situ hybridization with DNA markers. Genetica 131:267–274CrossRefPubMedGoogle Scholar
  14. Gold JR, Karel WJ, Strand MR (1980) Chromosome formulae of North American Fishes. Prog Fish Cult 42:10–23CrossRefGoogle Scholar
  15. Greenfield DW (1997) Allenbatrachus, a new genus of Indo-Pacific toadfish (Batrachoididae). Pac Sci 51:306–313Google Scholar
  16. Hatanaka T, Galetti PM Jr (2004) Mapping of the 18S and 5S ribosomal RNA genes in the fish Prochilodus argenteus Agassiz, 1829 (Characiformes, Prochilodontidae). Genetica 122:239–244CrossRefPubMedGoogle Scholar
  17. Hsu TC, Patak S, Chen TR (1975) The possibility of latent centromeres and a proposed nomenclature system for total chromosome and whole arm translocations. Cytogenet Cell Genet 15:41–49CrossRefPubMedGoogle Scholar
  18. Ijdo JW, Wells RA, Baldini A, Reeders ST (1991) Improved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR. Nucleic Acids Res 19:4780CrossRefPubMedGoogle Scholar
  19. Jones KW, Singh L (1985) Snakes and evolution of sex chromosomes. Trends Genet 1:55–61CrossRefGoogle Scholar
  20. Komiya H, Hasegawa M, Takemura S (1986) Differentiation of oocyte- and somatic-type 5S rRNAs in animals. J Biochem 100:369–374PubMedGoogle Scholar
  21. Magtoon W, Donsakul T (2008) Karyotytes of five teleostean fishes from Thailand. In: 34th Congress on science and technology of Thailand, BangkokGoogle Scholar
  22. Manchado M, Zuasti E, Cross I, Merlo A, Infante C, Rebordinos L (2006) Molecular characterization and chromosomal mapping of the 5S rRNA gene in Solea senegalensis: a new linkage to the U1, U2, and U5 small nuclear RNA genes. Genome 49:79–86CrossRefPubMedGoogle Scholar
  23. Martins C, Galetti PM Jr (1999) Chromosomal localization of 5S rDNA genes in Leporinus fish (Anostomidae, Characiformes). Chromosome Res 7:363–367CrossRefPubMedGoogle Scholar
  24. Martins C, Galetti PM Jr (2000) Conservative distribution of 5S rDNA loci in Schizodon (Pisces, Anostomidae) chromosomes. Chromosome Res 8:353–355CrossRefGoogle Scholar
  25. Martins C, Galetti PM Jr (2001) Two 5S arrays in Neotropical fish species: is it a general rule for fishes? Genetica 111:439–446CrossRefPubMedGoogle Scholar
  26. Martins C, Wasko AP, Oliveira C, Porto-Foresti F, Parise-Maltempi PP, Wright JM, Foresti F (2002) Dynamics of 5S rDNA in the tilapia (Oreochromis niloticus) genome: repeat units, inverted sequences, pseudogenes and chromosome loci. Cytogenet Genome Res 98:78–85CrossRefPubMedGoogle Scholar
  27. Matera AG, Weiner AM, Schmid CW (1990) Structure and evolution of the U2 small nuclear RNA multigene family in primates: gene amplification under natural selection? Mol Cell Biol 10:5876–5882PubMedGoogle Scholar
  28. Merlo MA, Cross I, Sarasquete C, Palazón-Fernández JL, Rebordinos L (2005) Caracterización cromosómica del pez sapo Halobatrachus didactylus (Schneider. 1801) (Teleostei: Batrachoididae) mediante hibridación in situ de fluorescencia. Bol Inst Eso Oceanogr 21:239–246Google Scholar
  29. Merlo A, Cross I, Palazón JL, Sarasquete C, Rebordinos L (2007) Chromosomal mapping of the major and minor ribosomal genes, (GATA)n and (TTAGGG)n by one-color and double color FISH in the toadfish Halobatrachus didactylus (Teleostei: Batrachoididae). Genetica 131:195–200CrossRefPubMedGoogle Scholar
  30. Merlo MA, Pacchiarini T, Sánchez-Ramos I, Chairi H, Cross I, Rebordinos L (2008) Utilización de las familias multigénicas para la diferenciación de especies de interés en Acuicultura. V Seminario de citogenética, Cádiz (Spain)Google Scholar
  31. Modesto T, Canário AVM (2003) Morphometric changes and sex steroid levels during the annual reproductive cycle of the Lusitanian toadfish, Halobatrachus didactylus. Gen Comp Endocrinol 131:220–231CrossRefPubMedGoogle Scholar
  32. Moscone EA, Klein F, Lambrou M, Fuchs J, Schweizer D (1999) Quantitative karyotyping and dual-color FISH mapping of 5S and 18S–25S rDNA probes in the cultivated Phaseolus species (Leguminosae). Genome 42:1224–1233CrossRefPubMedGoogle Scholar
  33. Nelson JS (1994) Fishes of the world. Wiley, New YorkGoogle Scholar
  34. Nirchio M, Gómez JA, Villalaz J (2001) Cariotipo del pez sapo Batrachoides pacifici (Batrachoididae: Teleostei) de la costa del Pacífico de Panamá. Saber Univer Oriente Venezuela 13:82–84Google Scholar
  35. Nirchio M, Turner BJ, Pérez JE, Gaviria JI, Cequea H (2002) Karyotypes of three species of toadfish (Batrachoididae: Teleostei) from Venezuela. Sci Mar 66:1–4CrossRefGoogle Scholar
  36. Nirchio M, Fenocchio AS, Swarça AC, Dias AL, Giuliano-Caetano L, Ron E, Gaviria JI, Pérez JE (2004a) Cytogenetic Characterization on Thalassophryne maculosa Günter, 1861 (Pises: Batrachoididae) from Margarita Island, Venezuela. Caribb J Sci 40:218–222Google Scholar
  37. Nirchio M, Fenocchio AS, Swarça AC, Pérez JE (2004b) Karyology of the toadfish Porichthys plectrodon (Jordan and Gilbert, 1882) (Batrachoididae) from Margarita Island, Venezuela. Mar Biol 146:161–165CrossRefGoogle Scholar
  38. Palazón JL, Nirchio M, Sarasquete C (2003) Conventional karyotype and nucleolar organiser regions of the toadfish Halobatrachus didactylus (Schneider, 1801) (Pisces: Batrachoididae). Sci Mar 67:445–449CrossRefGoogle Scholar
  39. Pelliccia F, Di Castro M, Lanza V, Volpi EV, Rocchi A (1991) GATA repeats in the genome of Asellus aquaticus (Crustacea, Isopoda). Chromosoma 100:152–155CrossRefPubMedGoogle Scholar
  40. Pelliccia F, Barzotti R, Bucciarelli E, Rocchi A (2001) 5S ribosomal and U1 small nuclear RNA genes: A new linkage type in the genome of a crustacean that has three different tandemly repeated units containing 5S ribosomal DNA sequences. Genome 44:331–335CrossRefPubMedGoogle Scholar
  41. Phillips RB (2001) Application of fluorescence in situ hybridization (FISH) to fish genetics and genome mapping. Mar Biotechnol 3:145–152CrossRefGoogle Scholar
  42. Rocco L, Costagliola D, Fiorillo M, Tinti F, Stingo V (2005) Molecular and chromosomal analysis of ribosomal cistrons in two cartilaginous fish, Taeniura lymma and Raja montagui (Chondrichthyes, Batoidea). Genetica 123:245–253CrossRefPubMedGoogle Scholar
  43. Schmidt M (1978) Chromosome banding in Amphibia. II. Constitutive heterochromatin and nucleolus organizer regions in Ranidae, Microhylidae and Racophoridae. Chromosoma 68:131–148CrossRefGoogle Scholar
  44. Singh L, Jones KW (1982) Sex reversal in the mouse (Mus musculus) is caused by a recurrent nonreciprocal crossover involving the X and an aberrant Y chromosome. Cell 28:205–216CrossRefPubMedGoogle Scholar
  45. Singh L, Jones KW (1986) Bkm sequences are polymorphic in humans and are clustered in pericentric regions of various acrocentric chromosomes including the Y. Hum Genot 73:304–308CrossRefGoogle Scholar
  46. Singh L, Purdom IF, Jones KW (1980) Sex chromosome associated satellite DNA: evolution and conservation. Chromosoma (Berl) 79:137–157CrossRefGoogle Scholar
  47. Subramanian S, Mishra RK, Singh L (2003) Genome-wide analysis of Bkm sequences (GATA repeats): predominant association with sex chromosomes and potential role in higher order chromatin organization and function. Bioinf Discov Note 19:681–685CrossRefGoogle Scholar
  48. Tiersch TR, Simco BA, Davis KB, Wachtel SS (1992) Molecular genetics of sex determination in a channel catfish: studies on SRY, XFY, Bkm and human telomeric repeats. Biol Reprod 47:185–192CrossRefPubMedGoogle Scholar
  49. Traut W (1987) Hypervariable Bkm DNA loci in a moth Ephesia kuehniella: does transposition cause restriction fragment length polymorphism? Genetics 115:112–118Google Scholar
  50. Vitturi R, Catalano E, Colomba MS, Montagnino L, Pellerito L (1995) Karyotype analysis of Aphanius fasciatus (Pisces: Cyprinodontiformes): Ag-NORs and C-band polymorphism in four populations from Sicily. Biol Zent 114:392–402Google Scholar
  51. Weigant J, Ried T, Nederlof PM, van der Ploeg M, Tanke HJ, Raap AK (1991) In situ hybridization with fluoresceinated DNA. Nucleic Acids Res 19:3237–3241CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • María Úbeda-Manzanaro
    • 1
  • Manuel A. Merlo
    • 2
  • José L. Palazón
    • 3
  • Ismael Cross
    • 2
  • Carmen Sarasquete
    • 1
  • Laureana Rebordinos
    • 2
  1. 1.Instituto de Ciencias Marinas de AndalucíaCSICPuerto RealSpain
  2. 2.Laboratorio Genética, Facultad de Ciencias del Mar y AmbientalesUniversidad de CádizPuerto RealSpain
  3. 3.Instituto de Investigaciones CientíficasUniversidad de OrienteIsla MargaritaVenezuela

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