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Genetica

, Volume 138, Issue 2, pp 169–177 | Cite as

Elucidating genetic relationships, diversity and population structure among the Turkish female figs

  • Hatice Ikten
  • Nedim Mutlu
  • Osman GulsenEmail author
  • Hilmi Kocatas
  • Uygun Aksoy
Article

Abstract

A collection of 96 female Turkish fig (Ficus carica L.) accessions was studied to elucidate genetic structure and estimate diversity and genetic similarity distribution among the female figs present in Turkish genetic resources, using 157 molecular genome markers including 129 sequence-related amplified polymorphisms, 21 random amplified polymorphic DNAs, and 7 simple-sequence repeats. The plant samples mainly included Turkish fig collections selected throughout the country over the course of a half-century. Neighbor-joining analysis revealed continuous dissimilarity range, and it was difficult to classify figs into distinct groups. The principle component analysis produced similar results. The analysis of molecular variance indicated that 95 and 93% of genetic variation were explained by within geographic origins and similar fruit rind color, respectively. Sub-structuring Bayesian analysis assigned the 96 female figs into four sub-populations, and indicated that they were highly related. The corrected allelic pairwise distances among the six geographic origins were less than 5%. This study suggests that geography- and color-based groups were not genetically distinct among the Turkish figs.

Keywords

Ficus carica SRAP Neighbor-joining PCA AMOVA Population structure 

Notes

Acknowledgments

This research was supported by a grant from The Scientific and Technological Research Council of Turkey, TUBITAK, (TOVAG-105O056). We are very grateful to Dr. Moshe A. Flaishman, the Volcani Center, Israel for providing part of research materials.

References

  1. Barkley NA, Roose ML, Krueger RR, Federici CT (2006) Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs). Theor Appl Genet 112:1519–1531CrossRefPubMedGoogle Scholar
  2. Budak H, Shearman RC, Gulsen O, Dweikat I (2005) Understanding ploidy complex and geographic origin of the Buffalograss genome using cytoplasmic and nuclear marker systems. Theor Appl Genet 111:1545–1552CrossRefPubMedGoogle Scholar
  3. Cabrita FL, Aksoy U, Hepaksoy S, Leitao MJ (2001) Suitability of isoenzyme, RAPD and AFLP markers to assess genetic differences and relatedness among fig (Ficus carica L.) clones. Sci Hortic 87:261–273CrossRefGoogle Scholar
  4. Chatti K, Salhi-Hannachi A, Mars M, Marrakchi M, Trifi M (2004) Genetic diversity and phylogenetic relationships in Tunisian fig (Ficus carica L.) cultivars mediated by RAPD. Biologia 2:1–4Google Scholar
  5. Chatti K, Saddoud O, Salhi-Hannachi A, Mars M, Marrakchi M, Trifi M (2007) Analysis of genetic diversity and relationships in a Tunisian Fig (Ficus carica) germplasm collection by random amplified microsatellite polymorphisms. J Integr Plant Biol 49:386–391CrossRefGoogle Scholar
  6. De Masi L, Castaldo D, Galano G, Minasi P (2005) Genotyping of fig (Ficus carica L.) via RAPD markers. Sci Food Agric 85:2235–2242CrossRefGoogle Scholar
  7. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
  8. Eroglu AS (1982) Fig research project: selection (in Turkish). Erbeyli Agricultural Research Institute, Aydin, Turkey (Unpublished report)Google Scholar
  9. Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distance among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491PubMedGoogle Scholar
  10. FAO (Food and Agricultural Organization) (2005) www.fao.org
  11. Fehr WR (1993) Heterozygosity. In: Principles of Cultivar Development: Theory and Technique. Vol. 1. MacGraw Hill, Inc, NY, pp. 115–119Google Scholar
  12. Fukunaga K, Hill J, Vigouroux Y, Matsuoka Y, Sanchez GJ, Liu K, Buckler ES, Doebley J (2005) Genetic diversity and population structure of Teosinte. Genetics 169:2241–2254CrossRefPubMedGoogle Scholar
  13. Garcia AF, Benchimol LL, Barbosa AMM, Geraldi IO, Souza CL Jr, de Souza AP (2004) Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines. Genet Mol Biol 27:579–588CrossRefGoogle Scholar
  14. Gulsen O, Roose ML (2001) Lemons: diversity and relationships with selected Citrus genotypes as measured with nuclear genome markers. J Am Soc Hort Sci 126:309–317Google Scholar
  15. Gulsen O, Shearman RC, Vogel KP, Lee DJ, Baenziger PS, Heng-Moss TM, Budak H (2005) Nuclear genome diversity and relationships among naturally occurring Buffalograss genotypes determined by sequence-related amplified polymorphism. HortScience 40:537–541Google Scholar
  16. Hudson RR, Hudson M, Maddison WP (1992) Estimation of levels of gene flow from DNA sequence data. Genetics 132:583–589PubMedGoogle Scholar
  17. Khadari B, Hochu I, Santoni S, Kjellberg F (2001) Identification and characterization of microsatellite loci in the common fig (Ficus carica L.) and representative species of genus Ficus. Mol Ecol Notes 1:191–193CrossRefGoogle Scholar
  18. Khadari B, Grout C, Santoni S, Kjellberg F (2005) Contrasted genetic diversity and differentiation among Mediterranean populations of Ficus carica L.: a study using mtDNA RFLP. Genet Res Crop Evol 52:97–109CrossRefGoogle Scholar
  19. Kjellberg F, Gouyon PH, Ibrahim M, Raymond M, Valdeyron G (1987) The stability of the symbiosis between dioecious figs and their pollinators: a study of Ficus carica L. and Blastophaga psenes L. Evol 41:693–704CrossRefGoogle Scholar
  20. Li G, Quiros CF (2001) Sequence related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103:455–461CrossRefGoogle Scholar
  21. Mars M, Chebli T, Marrakchi M (1998) Multivariate analysis fig (Ficus carica L.) germplasm in southern Tunisia. Acta Hort 480:75–81Google Scholar
  22. Mohammadi SA, Prasanna BM (2003) Analysis of genetic diversity in crop plants-salient statistical tools and considerations. Crop Sci 43:1235–1248CrossRefGoogle Scholar
  23. Ozbek S (1949) A research on Aegean fig cultivars (in Turkish). Ankara Univ J 98:1–15Google Scholar
  24. Papadopoulou K, Ehaliotis C, Tourna M, Kastanis P, Karydis I, Zervakis G (2002) Genetic relatedness among dioecious Ficus carica L. cultivars by random amplified polymorphic DNA analysis, and evaluation of agronomic and morphological characters. Genetica 114:183–194CrossRefPubMedGoogle Scholar
  25. Phan ATP (2000) Genetic diversity of blue grama (Bouteloua gracilis) and little bluestem (Schizachyrium scoparium) as affected by selection. Ph.D. Thesis, University of Manitoba, Winnipeg, MB, CanadaGoogle Scholar
  26. Pritchard KJ, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genet 155:945–959Google Scholar
  27. Rohlf FJ (1993) NTSYS-PC, numerical taxonomy and multivariate analysis system. Version 1.8. Exeter Software, Setauket, NYGoogle Scholar
  28. Rosenberg NA, Pritchard JK, Weber JL, Howard MC, Kidd KK, Zhivotovsky LA, Feldman MW (2002) Genetic structure of human populations. Science 298:2381–2385CrossRefPubMedGoogle Scholar
  29. Saddoud O, Chatti K, Salhi HA, Mars M, Rhouma A, Marrakchi M, Trifi M (2007) Genetic diversity of Tunisian figs (Ficus carica L.) as revealed by nuclear microsatellites. Hereditas 144:149–157CrossRefPubMedGoogle Scholar
  30. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–423PubMedGoogle Scholar
  31. Salhi-Hannachi A, Chatti K, Mars M, Marrakchi M, Trifi M (2005) Comparative analysis of genetic diversity in two Tunisian collections of fig cultivars based on random amplified polymorphic DNA and inter simple-sequence repeats fingerprints. Genet Res Crop Evol 52:563–573CrossRefGoogle Scholar
  32. Salhi-Hannachi A, Chatti K, Saddoud O, Mars M, Rhouma A, Marrakchi M, Trifi M (2006) Genetic diversity of different Tunisian fig (Ficus carica L.) collections revealed by RAPD fingerprint. Hereditas 143:15–22CrossRefPubMedGoogle Scholar
  33. Schneider S, Roessli D, Excoffier L (2001) Arlequin: a software for population genetics data analysis. Version 2.000. Genet and Biometry Lab, Department of Athropology, University of Geneva, GenevaGoogle Scholar
  34. Staub JE, Serquen FC, Gupta M (1996) Genetic markers, map construction, and their application in plant breeding. HortScience 31:729–741Google Scholar
  35. Valdeyron G, Lloyd DG (1979) Sex differences and flowering phenology in the common fig, Ficus carica L. Evol 33:673–685CrossRefGoogle Scholar
  36. Weiblen GD (2000) Phylogenetic relationships of functionally dioecious Ficus (Moraceae) based on ribosomal DNA sequences and morphology. Am J Bot 87:1342–1357CrossRefPubMedGoogle Scholar
  37. Zohary D, Hopf M (2000) Domestication of plants in the old world, 3rd edn. University Press, OxfordGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Hatice Ikten
    • 1
  • Nedim Mutlu
    • 2
  • Osman Gulsen
    • 3
    Email author
  • Hilmi Kocatas
    • 4
  • Uygun Aksoy
    • 5
  1. 1.West Mediterranean Research InstituteMinistry of Agriculture and Rural AffairsAntalyaTurkey
  2. 2.George W. Beadle Center, Department of BiochemistryUniversity of NebraskaLincolnUSA
  3. 3.Department of Horticulture, Faculty of AgricultureErciyes UniversityKayseriTurkey
  4. 4.The Fig Research InstituteAydinTurkey
  5. 5.Department of Horticulture, Faculty of AgricultureAegean UniversityIzmirTurkey

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