, 137:245 | Cite as

Chromosomal localization of 18S and 5S rDNA using FISH in the genus Tor (Pisces, Cyprinidae)

  • Mamta Singh
  • Ravindra Kumar
  • N. S. Nagpure
  • B. Kushwaha
  • Indramani Gond
  • W. S. Lakra


Dual color fluorescence in situ hybridization (FISH) was performed to study the simultaneous chromosomal localization of 18S and 5S ribosomal genes in the genus Tor for the first time. The 18S and 5S rDNAs in four Tor species were amplified, sequenced and mapped on the metaphase chromosomes. The number and distribution of 18S and 5S rDNA clusters were examined on metaphase chromosome spreads using FISH. The specimens of T. chelynoides, T. putitora and T. progeneius showed six bright fluorescent signals of 18S rDNA and T. tor exhibited ten such signals. The 5S rDNA signals were present only on one pair of chromosomes in all the four Tor species. Ag-NORs were observed on two pairs of chromosomes in T. chelynoides, T. putitora, T. progeneius and four pairs in T. tor. Comparison of the observed 18S rDNA FISH signals and Ag-NORs strongly suggested a possible inactivation of NORs localized at the telomeres of a subtelocentric and telocentric chromosome pairs in all four species. The 5S rDNA contained an identical 120 bp long coding region and 81 bp long highly divergent non-transcribed spacers in all species examined. 18S and 5S rDNA sequencing and chromosomal localization can be a useful genetic marker in species identification as well as phylogenetic and evolutionary studies.


18S 5S FISH rDNA Tor 



The authors are thankful to the Department of Biotechnology, the Ministry of Science and Technology, Government of India for funding.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Mamta Singh
    • 1
  • Ravindra Kumar
    • 1
  • N. S. Nagpure
    • 1
  • B. Kushwaha
    • 1
  • Indramani Gond
    • 1
  • W. S. Lakra
    • 1
  1. 1.National Bureau of Fish Genetic ResourcesLucknowIndia

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