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Microsatellite mutation rates in the eastern tiger salamander (Ambystoma tigrinum tigrinum) differ 10-fold across loci

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Abstract

Microsatellites are commonly used for mapping and population genetics because of their high heterozygosities and allelic variability (i.e., polymorphism). Microsatellite markers are generally more polymorphic than other types of molecular markers such as allozymes or SNPs because the insertions/deletions that give rise to microsatellite variability are relatively common compared to nucleotide substitutions. Nevertheless, direct evidence of microsatellite mutation rates (MMRs) is lacking in most vertebrate groups despite the importance of such estimates to key population parameters (e.g., genetic differentiation or θ = 4N e μ). Herein, we present empirical data on MMRs in eastern tiger salamanders (Ambystoma tigrinum tigrinum). We conducted captive breeding trials and genotyped over 1,000 offspring at a suite of microsatellite loci. These data on 7,906 allele transfers provide the first direct estimates of MMRs in amphibians, and they illustrate that MMRs can vary by more than an order of magnitude across loci within a given species (one locus had ten mutations whereas the others had none).

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Acknowledgments

This research was funded by a grant from the National Science Foundation (DEB-0514815 to J.A.D.). S. Hucko, B. Reinhart, and G. Stevens provided valuable assistance, and we thank them. We also thank the Department of Ecology and Evolutionary Biology at Cornell University for hosting J.A.D. during the preparation of this manuscript.

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Correspondence to J. Andrew DeWoody.

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Bulut, Z., McCormick, C.R., Gopurenko, D. et al. Microsatellite mutation rates in the eastern tiger salamander (Ambystoma tigrinum tigrinum) differ 10-fold across loci. Genetica 136, 501–504 (2009). https://doi.org/10.1007/s10709-008-9341-z

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  • DOI: https://doi.org/10.1007/s10709-008-9341-z

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