, Volume 135, Issue 1, pp 1–5 | Cite as

Classical and molecular cytogenetic characterization of Agonostomus monticola, a primitive species of Mugilidae (Mugiliformes)

  • Mauro Nirchio
  • Claudio Oliveira
  • Irani A. Ferreira
  • Cesar Martins
  • Anna Rita Rossi
  • Luciana Sola


This study reports the first description of the karyotype of Agonostomus monticola, a species belonging to a genus which is considered to be the most primitive among living mugilid fish. Specimens from Panama and Venezuela were cytogenetically analysed by conventional chromosome banding (Ag and base-specific-fluorochrome staining, C-banding) and by fluorescent in situ hybridization (FISH). Agonostomus monticola showed a chromosome complement of 2n = 48, composed of 23 acrocentric and one subtelocentric chromosome pairs and a pericentromeric distribution of the C-positive heterochromatin in all chromosomes. Major ribosomal genes were found to be located on the short arms of the subtelocentric chromosome pair number 24 and minor ribosomal genes in a paracentromeric position of a single medium-sized chromosome pair. All these observed cytogenetic features are similar to those previously described in four representatives of two genera, Liza and Chelon, which are considered to be among the most advanced in the family. Thus, this karyotypic form might represent the plesiomorphic condition for the mullets. This hypothesis regarding the plesiomorphic condition, if confirmed, would shed new light on the previously inferred cytotaxonomic relationships for the studied species of Mugilidae, because the karyotype with 48 acrocentric chromosomes, which has been so far regarded as primitive for the family, would have to be considered as derived.


Constitutive heterochromatin FISH Karyotype 18S and 5S rDNA NORs 



Financial support was provided by the Italian Ministry of Instruction, University and Research (MIUR), DM n. 219, 22-12-05, National Relevant Interest Research Projects (PRIN), 2005; by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) Brazil and by Consejo de Investigación, Universidad de Oriente, Venezuela.


  1. Almeida-Toledo LF, Ozouf-Costaz C, Foresti F, Bonillo C, Porto-Foresti F, Daniel-Silva MFZ (2002) Conservation of the 5S-bearing chromosome pair and co-localization with major rDNA clusters in five species of Astyanax (Pisces, Characidae). Cytogenet Genome Res 97:229–233PubMedCrossRefGoogle Scholar
  2. Caldara F, Bargelloni L, Ostellari L, Penzo E, Colombo L, Patarnello T (1996) Molecular phylogeny of grey mullets based on mitochondrial DNA sequence analysis: evidence of a differential rate of evolution at the intrafamily level. Mol Phylogenet Evol 6:416–424PubMedCrossRefGoogle Scholar
  3. Fujiwara A, Abe S, Yamaha E, Yamazaki F, Yoshida MC (1998) Chromosomal localization and heterochromatin association of ribosomal regions in salmonid fishes. Chromosome Res 6:463–471PubMedCrossRefGoogle Scholar
  4. Fujiwara A, Fujiwara M, Nishida-Umehara C, Abe S, Masaoka T (2007) Characterization of Japanese flounder karyotype by chromosome bandings and fluorescence in situ hybridization with DNA markers. Genetica 131: 267–274PubMedCrossRefGoogle Scholar
  5. Gromicho M, Ozouf-Costaz C, Collares-Pereira MJ (2005) Lack of correspondence between CMA3-, Ag-positive signals and 28S rDNA loci in two Iberian minnows (Teleostei, Cyprinidae) evidenced by sequential banding. Cytogenet Genome Res 109:507–511PubMedCrossRefGoogle Scholar
  6. Howell WM, Black DA (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36:1014–1015PubMedCrossRefGoogle Scholar
  7. Kavalco KF, Pazza R, Bertollo LAC, Moreira-Filho O (2005) Molecular cytogenetics of Oligosarcus hepsetus (Teleostei, Characiformes) from two Brazilian locations. Genetica 124:85–91PubMedCrossRefGoogle Scholar
  8. LeGrande WH, Fitzsimons JM (1976) Karyology of the mullets Mugil curema and Mugil cephalus (Perciformes: Mugilidae) from Louisiana. Copeia 1976:388–391CrossRefGoogle Scholar
  9. Martins C, Galetti Jr PM (1999) Chromosomal localization of 5S rDNA genes in Leporinus fish (Anostomidae, Characiformes). Chromosome Res 7:363–367PubMedCrossRefGoogle Scholar
  10. Moran P, Martinez JL, Garcia-Vasquez, Pendas AM (1996) Sex chromosome linkage of 5S rDNA in raimbow trout (Oncorhynchus mykiss). Cytogenet Cell Genet 75:145–150PubMedCrossRefGoogle Scholar
  11. Nelson JS (2006) Fishes of the world, 4th edn. Wiley, Hoboken, New JerseyGoogle Scholar
  12. Nirchio M, Cequea H (1998) Karyology of Mugil liza and M. curema from Venezuela. Bol Invest Mar Cost 27:45–50Google Scholar
  13. Nirchio M, Cipriano RR, Cestari MM, Fenocchio AS (2005) Cytogenetical and morphological features reveal significant differences among Venezuelan and Brazilian samples of Mugil curema. Neotrop Ichthyol 3:107–110CrossRefGoogle Scholar
  14. Nirchio M, Oliveira C, Ferreira IA, Pérez JE, Gaviria JI, Harrison I, Rossi AR, Sola L (2007) Comparative cytogenetic and allozyme analysis of Mugil rubrioculus and Mugil curema (Teleostei: Mugilidae) from Venezuela. Interciencia 32:757–762Google Scholar
  15. Ohno S (1974) Protochordata, cyclostomata and pisces. In: John B (ed) Animal cytogenetics, vol. 4, Chordata 1. Gerbrüder Borntraeger, BerlinGoogle Scholar
  16. Papasotiropoulos V, Klossa-Kilia E, Kilias G, Alahiotis S (2002) Genetic divergence and phylogenetic relationships in grey mullets (Teleostei: Mugilidae) based on PCR-RFLP analysis of mtDNA segments. Biochem Genet 40:71–86PubMedCrossRefGoogle Scholar
  17. Phillip DAT (1993) Reproduction and feeding of the mountain mullet, Agonostomus monticola, in Trinidad, West Indies. Environ Biol Fishes 37:47–55CrossRefGoogle Scholar
  18. Pinkel D, Straume T, Gray JW (1986) Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83:2934–2938PubMedCrossRefGoogle Scholar
  19. Rab P, Crossman EJ, Reed KM, Rabova M (2002) Chromosomal characteristics of ribosomal DNA in two extant species of North American mudminnows Umbra pygmea and U. limi (Euteleostei: Umbridae). Cytogenet Genome Res 98:194–198PubMedCrossRefGoogle Scholar
  20. Rossi AR, Gornung E (2005) Cytogenetic analysis of three Italian populations of Coregonus lavaretus (Pisces, Salmoniformes) with chromosomal localization of major and minor ribosomal genes, and telomeric repeats. Hereditas 142:15–21PubMedCrossRefGoogle Scholar
  21. Rossi AR, Ungaro A, De Innocentiis S, Crosetti D, Sola L (2004) Phylogenetic analysis of mediterranean mugilids by allozymes and 16S mt-rRNA genes investigation: are the mediterranean species of Liza monophyletic? Biochem Genet 42:301–315PubMedCrossRefGoogle Scholar
  22. Rossi AR, Gornung E, Sola L, Nirchio M (2005) Comparative molecular cytogenetic analysis of two congeneric species, Mugil curema and M. liza, characterized by significant karyotype diversity. Genetica 125:2732CrossRefGoogle Scholar
  23. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar
  24. Sola L, Rossi AR, Iaselli V, Rasch EM, Monaco PJ (1992) Cytogenetics of bisexual/unisexual species of Poecilia. II. Analysis of heterochromatin and nucleolar organizer regions in Poecilia mexicana mexicana by C-banding and DAPI, quinacrine, chromomycin A3 and silver staining. Cytogenet Cell Genet 60:229–235PubMedCrossRefGoogle Scholar
  25. Sola L, Gornung E, Mannarelli ME, Rossi AR (2007) Chromosomal evolution in Mugilidae, Mugilomorpha: an overview. In: Pisano E, Ozouf-Costaz C, Foresti F, Kapoor BG (eds) Fish cytogenetics. Science Publishers, Enfield, NH, USA, pp 165–194Google Scholar
  26. Souza IL, Galian J, De la Rua P, Bertollo LAC, Moreira-Filho O (2001) Non-random distribution of the GC-rich heterochromatin and nucleolar rDNA sites on Astyanax scabripinnis chromosomes. Cytologia 66:85–91Google Scholar
  27. Sumner AT (1972) A simple technique for demonstrating centromeric heterocromatin. Exp Cell Res 75:304–306PubMedCrossRefGoogle Scholar
  28. Thomson JM (1997) The Mugilidae of the world. Mem Queensl Mus 41:457–562Google Scholar
  29. Turan C, Caliskan M, Kucuktas H (2005) Phylogenetic relationships of nine mullet species (Mugilidae) in the Mediterranean Sea. Hydrobiologia 532:45–51CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Mauro Nirchio
    • 1
  • Claudio Oliveira
    • 2
  • Irani A. Ferreira
    • 2
  • Cesar Martins
    • 2
  • Anna Rita Rossi
    • 3
  • Luciana Sola
    • 3
  1. 1.Escuela de Ciencias Aplicadas del Mar. Universidad de OrienteBoca de RioVenezuela
  2. 2.Departamento de Morfologia, Instituto de BiociênciasUniversidade Estadual PaulistaBotucatuBrazil
  3. 3.Department of Animal and Human BiologyUniversity of Rome “La Sapienza”RomeItaly

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