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Genetica

, Volume 135, Issue 1, pp 1–5 | Cite as

Classical and molecular cytogenetic characterization of Agonostomus monticola, a primitive species of Mugilidae (Mugiliformes)

  • Mauro Nirchio
  • Claudio Oliveira
  • Irani A. Ferreira
  • Cesar Martins
  • Anna Rita Rossi
  • Luciana Sola
Article

Abstract

This study reports the first description of the karyotype of Agonostomus monticola, a species belonging to a genus which is considered to be the most primitive among living mugilid fish. Specimens from Panama and Venezuela were cytogenetically analysed by conventional chromosome banding (Ag and base-specific-fluorochrome staining, C-banding) and by fluorescent in situ hybridization (FISH). Agonostomus monticola showed a chromosome complement of 2n = 48, composed of 23 acrocentric and one subtelocentric chromosome pairs and a pericentromeric distribution of the C-positive heterochromatin in all chromosomes. Major ribosomal genes were found to be located on the short arms of the subtelocentric chromosome pair number 24 and minor ribosomal genes in a paracentromeric position of a single medium-sized chromosome pair. All these observed cytogenetic features are similar to those previously described in four representatives of two genera, Liza and Chelon, which are considered to be among the most advanced in the family. Thus, this karyotypic form might represent the plesiomorphic condition for the mullets. This hypothesis regarding the plesiomorphic condition, if confirmed, would shed new light on the previously inferred cytotaxonomic relationships for the studied species of Mugilidae, because the karyotype with 48 acrocentric chromosomes, which has been so far regarded as primitive for the family, would have to be considered as derived.

Keywords

Constitutive heterochromatin FISH Karyotype 18S and 5S rDNA NORs 

Notes

Acknowledgments

Financial support was provided by the Italian Ministry of Instruction, University and Research (MIUR), DM n. 219, 22-12-05, National Relevant Interest Research Projects (PRIN), 2005; by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) Brazil and by Consejo de Investigación, Universidad de Oriente, Venezuela.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Mauro Nirchio
    • 1
  • Claudio Oliveira
    • 2
  • Irani A. Ferreira
    • 2
  • Cesar Martins
    • 2
  • Anna Rita Rossi
    • 3
  • Luciana Sola
    • 3
  1. 1.Escuela de Ciencias Aplicadas del Mar. Universidad de OrienteBoca de RioVenezuela
  2. 2.Departamento de Morfologia, Instituto de BiociênciasUniversidade Estadual PaulistaBotucatuBrazil
  3. 3.Department of Animal and Human BiologyUniversity of Rome “La Sapienza”RomeItaly

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