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Mapping of rRNA genes and telomeric sequences in Danube salmon (Hucho hucho) chromosomes using primed in situ labeling technique (PRINS)

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Abstract

In the current paper we described the application of primed in situ (PRINS) labeling approach for the chromosomal mapping of repetitive DNA sequences in Danube salmon (Hucho hucho) (2n = 82, NF = 112). PRINS was successfully performed with primers enabling amplification of 5S rRNA genes (minor rDNAs), NOR building DNA sequences (major rDNAs), and telomeric sequences. Two loci of 5S rRNA were observed on distinct chromosome pairs; the minor arrays were located interstitially on the long (q) arms of two large metacentrics (chromosomes No. 3) and the large clusters of 5S rDNAs were assigned to the short (p) arms of two subtelocentric chromosomes No. 18. Major rDNA clusters were observed on the p-arms of two submeta-subtelocentric chromosomes No. 10. These chromosomal areas were built with GC-rich chromatin what was proved in the course of chromomycin A3 (CMA3) staining performed sequentially. Major and minor rDNA families were not co-localized in the Danube salmon chromosomes.The distinct hybridization signals at the ends of all the chromosomes were provided in the course of PRINS with (CCCTAA) n primer. The chromosomal localization of rRNA genes and telomeric DNA sequences was discussed in the context of Salmonidae karyotype evolution.

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References

  • Abuin M, Clabby C, Martinez P, Goswami U, Flavin F, Wilkins NP, Houghton JA, Powell R, Sanchez L (1996) Localization of the repetitive telomeric sequences (TTAGGG) in four salmonid species. Genome 39:1035–1038

    Article  PubMed  CAS  Google Scholar 

  • Allendorf FW, Thorgaard GH (1984) Tetraploidy and the evolution of salmonid fishes. In: Turner BJ (ed.) Evolutionary Genetics of Fishes. Plenum Press, New York, pp. 1–54

    Google Scholar 

  • Alonso M, Fujiwara A, Yamaha E, Kimura S, Abe S (1999) Ribosomal RNA and silver-stained nucleolar organizer regions associated with heterochromatin in Alaskan char (Salvelinus malma) and chum salmon (Oncorhynchus keta). Hereditas 131:221–225

    Article  PubMed  CAS  Google Scholar 

  • Amemiya ChT, Gold JR (1986) Chromomycin A3 stains Nucleolus Organizer Regions of fish chromosomes. Copeia 1:226–231

    Article  Google Scholar 

  • De Lucchini S, Nardi I, Barsacchi G, Batistioni R, Andronico F (1993) Molecular cytogenetics of ribosomal (18S + 28S and 5S) DNA loci in primitive and advanced urodele amphibians. Genome 36:762–763

    Article  PubMed  CAS  Google Scholar 

  • Frolov SV, Frolova VN (2000) Polymorphism and karyotype divergence in taimens of the Hucho genus. Genetika 36:237–240

    PubMed  CAS  Google Scholar 

  • Fujiwara A, Abe S, Yamaha E,Yamazaki F, Yoshida MC (1998) Chromosomal localization and heterochromatin association of ribosomal regions in salmonid fishes. Chromosome Res 6:463–471

    Article  PubMed  CAS  Google Scholar 

  • Gromicho M, Ozouf-Costaz C, Collares-Pereira MJ (2005) Lack of correspondence between CMA3-, Ag-positive signals and 28S rDNA loci in two Iberian minnows (Teleostei, Cyprinidae) evidenced by sequential banding. Cytogenet Genome Res 109:507–511

    Article  PubMed  CAS  Google Scholar 

  • Hatanaka T, Galett Jr PM (2004) Mapping of the 18S and 5S ribosomal RNA genes in the fish Prochilodus argentus Agassiz, 1829 (Characiformes, Procholodontidae). Genetica 122:239–244

    Article  PubMed  CAS  Google Scholar 

  • Jankun M, Martinez P, Padro BG, Kirtiklis L, Ráb P, Rabova M, Sanchez L (2001) Ribosomal genes in Coregonid fishes (C.lavaretus, C. albula and C. peled) (Salmonidae): single and multiple nucleolus organizer regions. Heredity 87:672–679

    Article  PubMed  CAS  Google Scholar 

  • Jankun M, Ocalewicz K, Padro BG, Martinez P, Woznicki P, Sanchez L (2003a) Localization of 5S rRNA loci and nucleolus organizer regions (NOR) in three coregonid species (Salmonidae). Genetica 119:183–186

    Article  PubMed  CAS  Google Scholar 

  • Jankun M, Ocalewicz K, Pardo BG, Martinez P, Woznicki P, Sanchez L (2003b) Chromosomal characteristics of rDNA in Thymallus thymallus (Salmoniformes). Genetica 119:219–224

    Article  PubMed  CAS  Google Scholar 

  • Koch J, Kolvraa S, Petersen K, Gregersen N, Bolend L (1989) Oligonucleotide-priming methods for chromosome specific labelling of alpha satellite DNA in situ. Chromosoma 98:259–265

    Article  PubMed  CAS  Google Scholar 

  • Komiya H, Hasegawa M, Takemura S (1986) Differentiation of oocyte- and somatic-type 5S rRNAs in animals. J Biochem 100:369–374

    PubMed  CAS  Google Scholar 

  • Martins C, Galetti Jr PM (1999) Chromosomal localisation of 5S rDNA genes in Leprinus fish (Anastomidae, Characiformes). Chromosome Res 7:363–367

    Article  PubMed  CAS  Google Scholar 

  • Martins C, Galetti Jr PM (2001a) Two 5S rDNA arrays in Neotropical fish species: is it a general rule for fishes? Genetica 111:439–446

    Article  PubMed  CAS  Google Scholar 

  • Martins C, Galetti Jr PM (2001b) Organization of 5S rDNA in species of the fish Leporinus: two different genomic locations are characterized by distinct nontranscribed spacers. Genome 44:903–910

    Article  PubMed  CAS  Google Scholar 

  • Moran P, Martinez JL, Garcia-Vazquez E, Pendas AM (1996) Sex chromosome linkage of 5S rDNA in rainbow trout (Oncorhynchus mykiss). Cytogenet Cell Genet 75:145–150

    Article  PubMed  CAS  Google Scholar 

  • Ocalewicz K, Sliwinska A, Jankun M (2004) Autosomal localization of Interstitial Telomeric Site (ITS) in brook trout, Salvelinus fontinalis (Pisces, Salmonidae). Cytogenet Genome Res 105:79–82

    Article  PubMed  CAS  Google Scholar 

  • Pendas AM, Moran P, Garcia-Vazquez E (1993) Ribosomal RNA genes are interspersed hroughout a heterochromatic chromosome arm in Atlantic salmon. Cytogenet Cell Genet 63:128–130

    Article  PubMed  CAS  Google Scholar 

  • Pendas AM, Moran P, Freije JP, Garcia-Vazquez E (1994) Chromosomal mapping and nucleotide sequence of two tandem repeats of Atlantic salmon 5S rDNA. Cytogenet Cell Genet 67:31–36

    Article  PubMed  CAS  Google Scholar 

  • Phillips RB, Matsuoka MP, Reed KM (2002) Characterization of charr chromosomes using fluorescence in situ hybridization. Environ Biol Fish 64:223–228

    Article  Google Scholar 

  • Phillips RB, Ráb P (2001) Chromosome evolution in the Salmonidae (Pisces): an update. Biol Rev (Cambridge) 76:1–25

    CAS  Google Scholar 

  • Ráb P, Liehman P (1982) Chromosome Study of the Danube Salmon Hucho hucho (Pisces, Salmonidae). Folia Zool 31:181–190

    Google Scholar 

  • Ráb P, Roth P (1988) Cold-blooded vertebrates. In: Balicek P, Forejt J, Rubes J (eds) Methods of Chromosome Analysis. Cytogeneticka sekce Ceskoslovenske biologicke spolecnosti pri CSAV, Brno Czech Republic, pp. 115–124

    Google Scholar 

  • Ráb P, Slechta V, Flajshans M (1994) Cytogenetics, cytotaxonomy and biochemical genetics of Huchoninae salmonids. Folia Zool 43:97–107

    Google Scholar 

  • Reed KM, Phillips RB (1995) Molecular cytogenetic analysis of the double-CMA3 chromosome of lake trout, Salvelinus namaycush. Cytogenet Cell Genet 70:104–107

    Article  PubMed  CAS  Google Scholar 

  • Schmidtke J, Zenzes MT, Weiler C, Bross K, Engel W (1976) Gene action in fish of tertarploid origin. IV. Ribosomal DNA amount in clupeoid and salmonid fish. Biochem Genet 14:293–297

    Article  PubMed  CAS  Google Scholar 

  • Sofradzhija A (1979) The chromosomes of Hucho hucho (L.). Paper presented at the 3rd European Ichthyological Congress, Warsaw Poland

  • Stein J, Phillips RB, Devlin RH (2001) Identification of the Y chromosome in chinook salmon (Oncorhynchus tshawytscha). Cytogenet Cell Genet 92:108–110

    Article  PubMed  CAS  Google Scholar 

  • Suzuki H, Sakurai S, Matsuda Y (1996) Rat rDNA spacer sequences and chromosomal aasignment of the genes to the extreme terminal region of chromosome 19. Cytogenet Cell Genet 72:1–4

    Article  PubMed  CAS  Google Scholar 

  • Viktorovskij RM, Makoedov AN, Shevchishin AA (1985) The chromosomal sets of Brachymystax lenok and Hucho taimen and the divergency of the salmonid genera. Tsitologia 27:703–709 (in Russian with an English abstract)

    Google Scholar 

  • Woznicki P, Sanchez L, Martinez P, Pardo BG, Jankun M (2000) A population analysis of the structure and variability of NOR in Salmo trutta by Ag, CMA3 and ISH. Genetica 108:113–118

    Article  CAS  Google Scholar 

  • Zhang Q, Cooper RK, Tiersch TR (2000) Chromosomal location of the 28S ribosomal RNA gene of channel catfish by in situ polymerase chain reaction. J Fish Biol 56:388–397

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This study was supported by the project No. 0804.0206 financed by University of Warmia and Mazury in Olsztyn, Poland.

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Correspondence to K. Ocalewicz.

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Ocalewicz, K., Woznicki, P. & Jankun, M. Mapping of rRNA genes and telomeric sequences in Danube salmon (Hucho hucho) chromosomes using primed in situ labeling technique (PRINS). Genetica 134, 199–203 (2008). https://doi.org/10.1007/s10709-007-9225-7

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  • DOI: https://doi.org/10.1007/s10709-007-9225-7

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