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Genetica

, Volume 133, Issue 3, pp 261–267 | Cite as

Variation in size and location of the Ag-NOR in the Atlantic halibut (Hippoglossus hippoglossus)

  • K. Ocalewicz
  • D. J. Penman
  • I. Babiak
Article

Abstract

The distribution of differentially stained chromatin was studied in the Atlantic halibut (Hippoglossus hippoglossus) chromosomes (2n = 48). Four pairs of homologous chromosomes were identified using a combination of traditional cytogenetic staining techniques (Giemsa/DAPI/CMA3/Ag-NO3). Chromosome 1 showed a length polymorphism (1S-short, 1L-long isoforms of the chromosome 1) which was related to the variation of the size of the Ag-NORs. In one specimen the Ag-NOR was translocated from chromosome 1 into the telomeric region on the q-arm of the chromosome 2 forming a derivative chromosome der(2)t(1S;2)(q?;q?). Four Ag-NOR genotypes have been shown: 1S1S, 1S1L, 1L1L and 1S der(2)t(1S;2)(q?;q?). The chromosome rearrangements did not leave any interstitially located telomeric sequences and the telomeres were confined to the ends of the chromosomes. A single chromosomal location of 5S rDNA clusters was found using the PRINS technique. In the extended metaphase spreads two adjacent clusters of 5S rDNA could be seen on one chromosome while condensed chromatin gave a single hybridization signal. Double 5S rDNA signals on the same chromosome arm suggested paracentric inversion of the minor rDNA site. 5S rDNA clusters were not co-localized with Ag-NORs. Although female and male karyotypes were compared no sex related cytogenetic markers were found.

Keywords

Chromosomes DAPI NORs Polymorphism Atlantic halibut Sex 

Notes

Acknowledgments

This work was supported by research project no. 165272/S40 financed by the Research Council of Norway.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Department of IchthyologyUniversity of Warmia and Mazury in OlsztynOlsztynPoland
  2. 2.Department of Fisheries and Natural SciencesBodø University CollegeBodoNorway
  3. 3.Institute of AquacultureUniversity of StirlingStirlingUK

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