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A study of wing morphology and fluctuating asymmetry in interspecific hybrids between Drosophila buzzatii and D. koepferae

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Abstract

In this work we investigate the effect of interspecific hybridization on wing morphology using geometric morphometrics in the cactophilic sibling species D. buzzatii and D. koepferae. Wing morphology in F1 hybrids exhibited an important degree of phenotypic plasticity and differs significantly from both parental species. However, the pattern of morphological variation between hybrids and the parental strains varied between wing size and wing shape, across rearing media, sexes, and crosses, suggesting a complex genetic architecture underlying divergence in wing morphology. Even though there was significant fluctuating asymmetry for both, wing size and shape in F1 hybrids and both parental species, there was no evidence of an increased degree of fluctuating asymmetry in hybrids as compared to parental species. These results are interpreted in terms of developmental stability as a function of a balance between levels of heterozygosity and the disruption of coadaptation as an indirect consequence of genomic divergence.

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Abbreviations

FA:

Fluctuating asymmetry

DA:

Directional asymmetry

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Acknowledgments

The authors wish to thank E. Soto for help in part of the experiments reported in this paper and to J. N. Thompson jr. for comments that helped to improve this paper. This work was supported by grants of Universidad de Buenos Aires, Agencia Nacional de Promoción Científica y Técnica and Consejo Nacional de Investigaciones Científicas y Técnicas. VC and IS were recipients of postgraduate scholarships of UBA and CONICET, respectively when this work was written. JJF and EH are members of Carrera del Investigador Científico of CONICET.

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  1. Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, Buenos Aires, C1428 EHA, Argentina

    Valeria P. Carreira, Ignacio M. Soto, Juan J. Fanara & Esteban Hasson

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  1. Valeria P. Carreira
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  2. Ignacio M. Soto
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  3. Juan J. Fanara
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Correspondence to Valeria P. Carreira.

Appendix

Appendix

Table 1 F values of the ANOVAs conducted with the absolute value of the difference between right and left wings for centroid size and procrustes distance corrected by DA if present (see text for more details) testing for differences among genotypes (D. koepferae, D. buzzatii and interspecific hybrids) and cacti (Opuntia sulphurea and Trichocereus terschekii) in flies of both sexes in each one of the six successful crosses
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Carreira, V.P., Soto, I.M., Fanara, J.J. et al. A study of wing morphology and fluctuating asymmetry in interspecific hybrids between Drosophila buzzatii and D. koepferae . Genetica 133, 1–11 (2008). https://doi.org/10.1007/s10709-007-9176-z

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