Skip to main content

Advertisement

SpringerLink
Log in

Genetic variation in the endangered Anisodus tanguticus (Solanaceae), an alpine perennial endemic to the Qinghai-Tibetan Plateau

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

We used random amplified polymorphic DNA markers (RAPDs) to assess genetic variation between- and within-populations of Anisodus tanguticus (Solanaceae), an endangered perennial endemic to the Qinghai-Tibetan Plateau with important medicinal value. We recorded a total of 92 amplified bands, using 12 RAPD primers, 76 of which (P = 82.61%) were polymorphic, and calculated values of Ht and Hsp of 0.3015 and 0.4459, respectively, suggesting a remarkably high rate of genetic variation at the species level. The average within-population diversity also appeared to be high, with P, He and Hpop values of 55.11%, 0.1948 and 0.2918, respectively. Analyses of molecular variance (AMOVA) showed that among- and between-population genetic variation accounted for 67.02% and 32.98% of the total genetic variation, respectively. In addition, Nei’s coefficient of differentiation (GST) was found to be high (0.35), confirming the relatively high level of genetic differentiation among the populations. These differentiation coefficients are higher than mean corresponding coefficients for outbreeding species, but lower than reported coefficients for some rare species from this region. The genetic structure of A. tanguticus has probably been shaped by its breeding attributes, biogeographic history and human impact due to collection for medicinal purposes. The observed genetic variations suggest that as many populations as possible should be considered in any planned in situ or ex situ conservation programs for this species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Bolaric S, Barth S, Melchinger AE, Posselt UK (2005) Genetic diversity in European perennial ryegrass cultivars investigated with RAPD markers. Plant Breed 124:161–166

    Article  CAS  Google Scholar 

  • Borowsky R (2001) Estimating nucleotide diversity from random amplified polymorphic DNA and amplified fragment length polymorphism data. Mol Phylogenet Evol 18:143–148

    Article  PubMed  CAS  Google Scholar 

  • Bussell JD (1999) The distribution of random amplified polymorphic DNA (RAPD) diversity amongst populations of Isotoma petraea (Lobeliaceae). Mol Ecol 8:775–789

    Article  CAS  Google Scholar 

  • Carlson JE (1991) Segregation of random amplified DNA Markers in F1 progeny of conifers. Theor Appl Genet 83:194–200

    Article  Google Scholar 

  • Chen SL, Xia T, Chen SY, Zhou YJ (2005) RAPD Profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China. Biochem Genet 43:189–201

    Article  PubMed  CAS  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf material. Phytochem Bull 19:11–15

    Google Scholar 

  • Ellstand NC, Elam DR (1993) Population genetic consequences of small population size: implications for plant conservation. Annu Rev Ecol Syst 24:217–242

    Article  Google Scholar 

  • Ennos RA (1994) Estimating the relative rates of pollen and seed migration among plant populations. Heredity 72:250–259

    Google Scholar 

  • Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distance among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:176–191

    Google Scholar 

  • Gabrielsen TM, Bachmann K, Jakobsen KS, Brochmann C (1997) Glacial survival does not matter: RAPD phylogeography of Nordic Saxifraga oppositifolia. Mol Ecol 6:831–842

    Article  CAS  Google Scholar 

  • Ge XJ, Zhang LB, Yuan YM, Hao G, Chiang TY (2005) Strong genetic differentiation of the East-Himalayan Megacodon stylophorus (Gentianaceae) detected by inter-simple sequence repeats (ISSR). Biodivers Conserv 14:849–861

    Article  Google Scholar 

  • Hamrick JL, Godt MJW (1989) Allozyme diversity in plant species. In: Brown AHD, Clegg MT, Kahler AL, Weir BS (eds) Population genetics and germplasm resources in crop improvement. Sinauer Associates, Sunderland

    Google Scholar 

  • Hamrick JL, Godt MJW (1996) Conservation genetics of endemic plant species. In: Avise JC, Hamrick JL (eds) Conservation genetics: case histories from nature. Chapman & Hall, New York

    Google Scholar 

  • He YJ, Cui GF, Feng ZW, Zheng J, Dong JS, Li YB (2004) Conservation priorities for plant species of forest-meadow ecotone in Sanjiangyuan nature reserve. Chin J Appl Ecol 15(8):1307–1312

    Google Scholar 

  • Huff DR, Peakall R, Smouse PE (1993) RAPD variation within and among natural populations of outcrossing buffalograss (Buchloe dactyloides (Nutt.) Engelm). Theor Appl Genet 8:927–934

    Google Scholar 

  • Liu JQ, Gao TG, Chen ZD, Lu AM (2002) Molecular phylogeny and biogeography of the Qinghai-Tibet Plateau endemic Nannoglottis (Asteraceae). Mol Phylogenet Evol 23:307–325

    Article  PubMed  CAS  Google Scholar 

  • Liu JQ, Wang YJ, Wang AL, Ohba H, Abbott R (2006a) Radiation and diversification of Ligularia-Cremanthodium-Parasenecio triggered by uplifts of the Qinghai-Tibetan Plateau. Mol Phylogenet Evol 38:31–49

    Article  PubMed  CAS  Google Scholar 

  • Liu JM, Wang L, Geng YP, Wang QB, Luo LJ, Zhong Y (2006b) Genetic diversity and population structure of Lamiophlomis rotata (Lamiaceae), an endemic species of Qinghai–Tibet Plateau. Genetica 128:385–394

    Article  PubMed  Google Scholar 

  • Lynch M, Milligan GG (1994) Analysis of population genetic structure with RAPD markers. Mol Ecol 3:91–99

    PubMed  CAS  Google Scholar 

  • Miller MP (1997) Tools for population genetic analysis. Version 1.3. Department of Biological Sciences, Northern Arizona University, Flagstaff

  • Miller MP (1998) Amova-Prep 1.01. A program for the preparation of AMOVA input files from dominant-market raw data. Northern Arizona University, Flagstaff AZ. http:// herb.bio.nau.edu/miller/amovaprp.htm

  • Myers N, Mittermeier RA, Mittermeier CG, Fonseca da GAB, Kentm J (2000) Biodiversity hotspots for conservation priorities. Nature 403:853–858

    Article  PubMed  CAS  Google Scholar 

  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590

    PubMed  Google Scholar 

  • Newton AC, Allnutt TR, Gillies ACM, Lowe AJ, Ennos RA (1999) Molecular phylogeography, intraspecific variation and the conservation of tree species. Trends Ecol Evol 14:140–145

    Article  PubMed  Google Scholar 

  • Nybom H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol Ecol 13:1143–1155

    Article  PubMed  CAS  Google Scholar 

  • Peakall R, Smouse PE, Huff DR (1995) Evolutionary implications of allozyme and RAPD variation in diploid populations of dioecious buffalograss, Buchloe dactyloides. Mol Ecol 4:135–147

    CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (eds) (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Press, Cold Spring Harbor, NY

    Google Scholar 

  • Schaal BA, Hayworth DA, Olsen KM, Rauscher JT, Smith WA (1998) Phylogeographic studies in plants: problems and prospects. Mol Ecol 7:465–474

    Article  Google Scholar 

  • Wang AL, Yang MH, Liu JQ (2005) Molecular phylogeny, recent radiation and evolution of gross morphology of the rhubarb genus Rheum (Polygonaceae) inferred from chloroplast DNA trnL-F sequences. Ann Bot London 96:489–498

    Article  CAS  Google Scholar 

  • Weising K, Nybom H, Wolff K, Meyer W (eds) (1995) DNA fingerprinting in plants and fungi. CRC Press, Boca Raton

    Google Scholar 

  • Wolfe AD, Liston A (1998) Contributions of PCR-based methods to plant systematics and evolutionary biology. In: Soltis DE, Soltis PS, Doyle JJ (eds) Molecular systematics of plants II, DNA sequencing. Kluwer Academic Publishers, Dordrecht

    Google Scholar 

  • Xia T, Chen SL, Chen SY, Ge XJ (2005) Genetic variation within and among populations of Rhodiola alsia (Crassulaceae) native to the Tibetan Plateau as detected by ISSR markers. Biochem Genet 43:87–101

    Article  PubMed  CAS  Google Scholar 

  • Yang DZ, Zhang ZY, Lu AM, Sun K, Liu JQ (2002) Floral organogenesis and development of two taxa of the Solanaceae-Anisodus tanguticus and Atropa belladonna. Israel J Plant Sci 50:127–134

    Article  Google Scholar 

  • Yang YC (ed) (1991) Tibetan medicines. Qinghai People Press, Qinghai

    Google Scholar 

  • Yap IV, Nelson RJ (1996) WinBoot: a program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA-based dendrograms. International Rice Research Instititute (IRRI), Manila, Philippines

    Google Scholar 

  • Yeh FC, Yang R, Boyle T (1999) POPGENE. Microsoft Windows-based freeware for population genetic analysis. Release 1.31. University of Alberta, Edmonton, Canada

  • Zheng D (1996) The system of physico-geographical regions of the Qinghai-Tibet (Xizang) Plateau. Sci China Ser D 39:410–417

    Google Scholar 

Download references

Acknowledgements

This work was supported by grants from the Chinese Academy of Sciences (Key Innovation Plan KSCX2-SW-106 and the Special Fund for Outstanding PhD Dissertation).

Author information

Authors and Affiliations

  1. Key Laboratory of the Qinghai-Tibetan Plateau Ecosystem and Biological Evolution and Adaptation, Northwest Institute of Plateau Biology, The Chinese Academy of Sciences, Xining, Qinghai, 810001, P.R. China

    Wei Zheng, Liuyang Wang, Lihua Meng & Jianquan Liu

  2. Key Laboratory of Arid and Grassland Ecology, Lanzhou University, Lanzhou, Gansu, 730000, P.R. China

    Jianquan Liu

  3. Graduate School of the Chinese Academy of Sciences, Beijing, 100039, P.R. China

    Wei Zheng

Authors
  1. Wei Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Liuyang Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lihua Meng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jianquan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jianquan Liu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zheng, W., Wang, L., Meng, L. et al. Genetic variation in the endangered Anisodus tanguticus (Solanaceae), an alpine perennial endemic to the Qinghai-Tibetan Plateau. Genetica 132, 123–129 (2008). https://doi.org/10.1007/s10709-007-9154-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10709-007-9154-5

Keywords

Search

Navigation