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Isolation and characterization of sex chromosome rearrangements generating male muscle dystrophy and female abnormal oogenesis in the silkworm, Bombyx mori

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In deletion-mapping of W-specific RAPD (W-RAPD) markers and putative female determinant gene (Fem), we used X-ray irradiation to break the translocation-carrying W chromosome (WZe). We succeeded in obtaining a fragment of the WZe chromosome designated as Ze W, having 3 of 12 W-RAPD markers (W-Bonsai, W-Yukemuri-S, W-Yukemuri-L). Inheritance of the Ze W fragment by males indicates that it does not include the Fem gene. On the basis of these results, we determined the relative positions of W-Yukemuri-S and W-Yukemuri-L, and we narrowed down the region where Fem gene is located. In addition to the Ze W fragment, the Z chromosome was also broken into a large fragment (Z1) having the +sch (1-21.5) and a small fragment (Z2) having the +od (1-49.6). Moreover, a new chromosomal fragment (Ze WZ2) was generated by a fusion event between the Ze W and the Z2 fragments. We analyzed the genetic behavior of the Z1 fragment and the Ze WZ2 fragment during male (Z/Z1 Ze WZ2) and female (Z1 Ze WZ2/W) meiosis using phenotypic markers. It was observed that the Z1 fragment and the Z or the W chromosomes separate without fail. On the other hand, non-disjunction between the Ze WZ2 fragment and the Z chromosome and also between the Ze WZ2 fragment and the W chromosome occurred. Furthermore, the females (2A: Z/Ze WZ2/W) and males (2A: Z/Z1) resulting from non-disjunction between the Ze WZ2 fragment and the W chromosome had phenotypic defects: namely, females exhibited abnormal oogenesis and males were flapless due to abnormal indirect flight muscle structure. These results suggest that Z2 region of the Z chromosome contains dose-sensitive gene(s), which are involved in oogenesis and indirect flight muscle development.

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Acknowledgments

This work was supported by grants from BRAIN (to. K. M.), Grants-in-Aid for Scientific Research, MEXT (Nos. 17052006, 16011263, and 17018007) and JSPS (No. 16208006), the Insect Technology Project, MAFF/NIAS (No. 1216), and the National Bioresource Project, MEXT.

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Authors and Affiliations

  1. Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Saiwai-cho 3-5-8, Fuchu, Tokyo, 183-8509, Japan

    T. Fujii, T. Yokoyama, O. Ninagi, K. Kakehashi & H. Abe

  2. Department of Veterinary, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Saiwai-cho 3-5-8, Fuchu, Tokyo, 183-8509, Japan

    Y. Obara

  3. National Institute of Radiological Sciences, Anagawa 4-9-1, Inage-ku, Tiba, 263-8555, Japan

    M. Nenoi & T. Ishikawa

  4. National Institute of Agrobiological Science, Owashi 1-2, Tsukuba, Ibaraki, 305-8634, Japan

    K. Mita

  5. Department of Agricultural and Environmental Biology, Graduate school of Agricultual and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan

    T. Shimada

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  1. T. Fujii
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  9. T. Shimada
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Correspondence to H. Abe.

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Fujii, T., Yokoyama, T., Ninagi, O. et al. Isolation and characterization of sex chromosome rearrangements generating male muscle dystrophy and female abnormal oogenesis in the silkworm, Bombyx mori . Genetica 130, 267–280 (2007). https://doi.org/10.1007/s10709-006-9104-7

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  • DOI: https://doi.org/10.1007/s10709-006-9104-7

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