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Genetica

, 130:133 | Cite as

Physical mapping of 5S and 45S rDNA loci in pufferfishes (Tetraodontiformes)

  • Rafael Bueno Noleto
  • Marcelo Ricardo Vicari
  • Roger Raupp Cipriano
  • Roberto Ferreira Artoni
  • Marta Margarete Cestari
Original Paper

Abstract

Chromosomal features, location and variation of the major and minor rDNA genes cluster were studied in three pufferfish species: Sphoeroides greeleyi and Sphoeroides testudineus (Tetraodontidae) and Cyclichthys spinosus (Diodontidae). The location of the major rDNA was revealed with an 18S probe in two loci for all species. The minor rDNA loci (5S rDNA) was found in one chromosome pair in tetraodontid fishes and four sites located on two distinct chromosomal pairs in C. spinosus. A syntenical organization was not observed among the ribosomal genes. Signal homogeneity for GC/AT-DNA specific fluorochromes was observed in diodontid fish except in the NORs regions, which were CMA3-positive. Giemsa karyotypes of tetraodontid species presents 2n = 46, having the same diploid value of other Sphoeroides species that have been investigated. On the other hand, the karyotype of C. spinosus, described for the first time, shows 2n = 50 chromosomes (4m + 18sm + 12st + 16a). The foreknowledge of the karyotypic structure of this group and also the physical mapping of certain genes could be very helpful for further DNA sequence analysis.

Keywords

Pufferfish Tetraodontiformes 18S & 5S rDNA 

Notes

Acknowledgments

The authors thank Dr. Julio Cesar Pieczarka for the careful revision of the manuscript and Dr. Luiz Antonio Carlos Bertollo, Universidade Federal de São Carlos for thechnical assistance and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico). This research was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná.

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Rafael Bueno Noleto
    • 1
  • Marcelo Ricardo Vicari
    • 2
  • Roger Raupp Cipriano
    • 1
  • Roberto Ferreira Artoni
    • 3
  • Marta Margarete Cestari
    • 1
  1. 1.Departamento de Genética, Centro PolitécnicoUniversidade Federal do ParanáCuritibaBrazil
  2. 2.Departamento de Genética e EvoluçãoUniversidade Federal de São CarlosSão CarlosBrazil
  3. 3.Departamento de Biologia Estrutural, Molecular e GenéticaUniversidade Estadual de Ponta GrossaPonta GrossaBrazil

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