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Phenotypic effect of substitution of allelic variants for a histone H1 subtype specific for growing tissues in the garden pea (Pisum  sativum L.)

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Abstract

In pea, subtype H1-7 of histone H1 is specific for young actively growing tissues and disappears from chromatin of mature tissues. We sequenced the alleles coding for three main variants, numbered according to the increase of the electrophoretic mobility. Allele 1 differs from the most common allele 2 by eight nucleotide substitutions, two of them associated with amino acid replacements, His->Tyr in the globular domain and Ala->Val in the C-terminal domain. Allele 3 differs from alleles 1 and 2 by a 24-bp deletion in the part coding for the C-terminal domain. In three greenhouse experiments, we compared quantitative traits in nearly isogenic lines differing by these H1-7 variants. In experiment 1, three lines bearing either of the three allelic variants were compared, the other experiments involved pairs of lines bearing variants 1 and 3. In all experiments, statistically significant differences between the lines were registered, mostly related to the plant size. The most prominent effect was associated with plant growth dynamics. Plants of line 3, carrying the 8-amino acid deletion in histone H1-7, on average grew slower. In two experiments, the differences of the mean stem length persisted throughout plant growth while in experiment 2 differences disappeared upon maturity. The H1-7 subtype is supposed to be related to maintenance of chromatin state characteristic for cell growth and division.

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Acknowledgements

This work was supported by the Russian State Program ‘Russian Fund for Fundamental Research’, Grant No. 02-04-49426 and the “Biosphere origin and evolution” project of the Russian Academy of Sciences.

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  1. Institute of Cytology & Genetics, Acad. Lavrentiev ave. 10, Novosibirsk , 630090, Russia

    Vera S. Bogdanova, Oleg E. Kosterin & Vladimir A. Berdnikov

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  1. Vera S. Bogdanova
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  2. Oleg E. Kosterin
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  3. Vladimir A. Berdnikov
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Correspondence to Oleg E. Kosterin.

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Bogdanova, V.S., Kosterin, O.E. & Berdnikov, V.A. Phenotypic effect of substitution of allelic variants for a histone H1 subtype specific for growing tissues in the garden pea (Pisum  sativum L.). Genetica 130, 61–72 (2007). https://doi.org/10.1007/s10709-006-0021-6

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