Karyotype and Chromosomal Location of 18S–28S and 5S Ribosomal DNA in the Scallops Pecten maximus and Mimachlamys varia (Bivalvia: Pectinidae)

Abstract

This work describes the karyotype and chromosomal location of the ribosomal DNA (rDNA) of Pecten maximus and Mimachlamys varia, two commercial scallop species from Europe. According to the chromosome centromeric index values found, the karyotype of P. maximus is composed of 1 metacentric, 2 metacentric–submetacentric, 1 telocentric–subtelocentric and 15 telocentric pairs, and that of M. varia of 4 metacentric, 2 subtelocentric–submetacentric, 9 subtelocentric, 3 subtelocentric–telocentric and 1 telocentric–subtelocentric pairs. In P. maximus, 18S-28S rDNA was located by FISH on a metacentric–submetacentric pair, and in M. varia on a subtelocentric–submetacentric pair using both silver staining and FISH. PCR amplification of the 5S rDNA unit yielded a single product of about 460 bp (P. maximus) and 450 bp (M. varia), that used as probe revealed a 5S rDNA site on a telocentric pair in P. maximus and a subtelocentric pair in M. varia. Two-color FISH or sequential silver staining of 5S rDNA-FISH-metaphases corroborated that the two gene families are located on different chromosomes in both species. A comparative analysis of the data allowed the inference of karyotypic relationships within scallops.

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References

  1. J. Baron A. Diter A. Bodoy (1989) ArticleTitleTriploidy induction in the black scallop (Chlamys varia L.) and its effect on larval growth and survival Aquaculture 77 103–111 Occurrence Handle10.1016/0044-8486(89)90194-4

    Article  Google Scholar 

  2. E. Basoa C. Alfonsi J.E. Pérez H. Cequea (2000) ArticleTitleKaryotypes of the scallops Euvola ziczac and Nodipecten nodosus from the Gulf of Cariaco, Sucre state, Venezuela Bol. Inst. Oceanogr. Venezuela 39 49–54

    Google Scholar 

  3. A.R. Beaumont L.L.D. Gruffydd (1974) ArticleTitleStudies on the chromosomes of the scallop Pecten maximus (L.) and related species J. Mar. Biol. Ass. UK 54 713–718

    Google Scholar 

  4. Boss, K.J., 1982. Mollusca, pp. 945–1166 in Synopsis and classification of living organisms, edited by S.P. Parker McGraw-Hill, New York

  5. Brand, A.R., 1991. Scallop ecology: distributions and behaviour, pp. 515–584 in Scallops: Biology, Ecology and Aquaculture, edited by S.E. Shumway Elsevier Science Publishers, Amsterdam

  6. Colomba M.S., R. Vitturi, L. Castriota, R. Bertoni & A. Libertini, 2002. FISH mapping of 18S-28S and 5S ribosomal DNA, (GATA)n and (TTAGGG)n telomeric repeats in the periwinkle Melarhaphe neritoides (Prosobranchia, Gastropoda, Caenogastropoda). Heredity 88: 381–384

    Google Scholar 

  7. G. Drouin (1999) ArticleTitleHomogenization of 5S ribosomal genes on the noncoding strand of the rDNA units of two crustacean species Genome 42 150–153 Occurrence Handle10.1139/gen-42-1-150 Occurrence Handle1:CAS:528:DyaK1MXhsVyqtrg%3D

    Article  CAS  Google Scholar 

  8. G. Gajardo M. Parraguez N. Colihueque (2002) ArticleTitleKaryotype analysis and chromosome banding of the Chilean-Peruvian scallop Argopecten purpuratus (Lamarck, 1819) J. Shellfish Res. 21 585–590

    Google Scholar 

  9. J.R. Gold J.R. Ellison (1982) ArticleTitleSilver staining for nucleolar organizing regions of vertebrate chromosomes Stain Technol. 58 51–55

    Google Scholar 

  10. A.M. González-Tizón A. Martínez-Lage I. Rego J. Ausío J. Méndez (2000) ArticleTitleDNA content, karyotypes, and chromosomal location of 18S-5.8S-28S ribosomal loci in some species of bivalve molluscs from the Pacific Canadian coast Genome 43 1065–1072 Occurrence Handle11195339

    PubMed  Google Scholar 

  11. W.M. Howell (1977) ArticleTitleVisualization of ribosomal gene activity: silver stain proteins associated with rRNA transcribed from oocyte chromosomes Chromosoma 62 361–367 Occurrence Handle10.1007/BF00327034 Occurrence Handle70325 Occurrence Handle1:CAS:528:DyaE2sXls1ersL4%3D

    Article  PubMed  CAS  Google Scholar 

  12. W.M. Howell D.A. Black (1980) ArticleTitleControlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method Experientia 36 1014–1015 Occurrence Handle10.1007/BF01953855 Occurrence Handle6160049 Occurrence Handle1:STN:280:Bi6D28npslw%3D

    Article  PubMed  CAS  Google Scholar 

  13. H. Ieyama (1975) ArticleTitleChromosome number of three species in three families of Pteriomorphia (Bivalvia) Venus 34 26–32

    Google Scholar 

  14. A. Insua (1993) Estudio cariológico en moluscos bivalvos. PhD thesis Universidade da Coruña A Coruña, Spain

    Google Scholar 

  15. A. Insua J. Mendez (1998) ArticleTitlePhysical mapping and activity of ribosomal RNA genes in mussel Mytilus galloprovincialis Hereditas 128 189–194 Occurrence Handle10.1111/j.1601-5223.1998.00189.x Occurrence Handle9760868 Occurrence Handle1:CAS:528:DyaK1cXmsVKjsr4%3D

    Article  PubMed  CAS  Google Scholar 

  16. A. Insua M.J. López-Piñón J. Méndez (1998) ArticleTitleCharacterization of Aequipecten opercularis (Bivalvia: Pectinidae) chromosomes by different staining techniques and fluorescent in situ hybridization Genes Genet. Syst. 73 193–200 Occurrence Handle10.1266/ggs.73.193 Occurrence Handle9880917 Occurrence Handle1:STN:280:DyaK1M%2FpslGqsA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  17. A. Insua R. Freire J. Méndez (1999) ArticleTitleThe 5S rDNA of the bivalve Cerastoderma edule: nucleotide sequence of the repeat unit and chromosomal location relative to 18S–28S rDNA Genet. Sel. Evol. 31 509–518 Occurrence Handle1:CAS:528:DC%2BD3cXhtVWlur8%3D

    CAS  Google Scholar 

  18. A. Insua R. Freire J. Ríos J. Méndez (2001) ArticleTitleThe 5S rDNA of mussels Mytilus galloprovincialis and M. edulis: sequence variation and chromosomal location Chromosome Res. 9 495–505 Occurrence Handle10.1023/A:1011636714052 Occurrence Handle11592484 Occurrence Handle1:CAS:528:DC%2BD3MXnt1ektbk%3D

    Article  PubMed  CAS  Google Scholar 

  19. A. Insua M.J. López-Piñón R. Freire J. Méndez (2003) ArticleTitleSequence analysis of the ribosomal DNA internal transcribed spacer region in some scallop species (Mollusca: Bivalvia: Pectinidae) Genome 46 595–604 Occurrence Handle10.1139/g03-045 Occurrence Handle12897868 Occurrence Handle1:CAS:528:DC%2BD3sXnsVertrw%3D

    Article  PubMed  CAS  Google Scholar 

  20. R. Kilias (1982) Mollusca Bivalvia H.J. Gruner (Eds) Lehrbuch der Speziellen Zoologie Gustav Fischer Verlag Stuttgart 152–202

    Google Scholar 

  21. A. Komaru K.T. Wada (1985) ArticleTitleKaryotypes of four species in the Pectinidae (Bivalvia: Pteriomorphia) Venus 44 249–259

    Google Scholar 

  22. M.V. Kost A.A. Alimov A.G. Sarafanov T.P. Tikchomirova R.R. Gumeniuk M.Ja. Timofeeva A.V. Zelenin (1995) ArticleTitle5S rRNA gene hybridizes to human chromosome 1 at two sites (1q42.11→q42.13 and 1q43) Cytogenet. Cell Genet. 68 82–84 Occurrence Handle7956368 Occurrence Handle1:CAS:528:DyaK2MXjtV2rurg%3D

    PubMed  CAS  Google Scholar 

  23. A. Leitão P. Boudry J.P. Labat C. Thiriot-Quiévreux (1999) ArticleTitleComparative karyological study of cupped oyster species Malacologia 41 175–186

    Google Scholar 

  24. A. Leitão R. Chaves S. Santos P. Boudry H. Guedes-Pinto C. Thiriot-Quiévreux (2002) ArticleTitleCytogenetic study of Ostrea conchaphila (Mollusca: Bivalvia) and comparative karyological analysis within Ostreinae J. Shellfish Res. 21 685–690

    Google Scholar 

  25. A. Levan K. Fredga A.A. Sandberg (1964) ArticleTitleNomenclature for centromeric position on chromosomes Hereditas 52 201–220

    Google Scholar 

  26. W.H. Li (1997) Molecular evolution Sinauer Associates Sunderland

    Google Scholar 

  27. A.R. Linares T. Bowen G.A. Dover (1994) ArticleTitleAspects of nonrandom turnover involved in the concerted evolution of intergenic spacers within the ribosomal DNA of Drosophila melanogaster J. Mol. Evol. 39 151–159 Occurrence Handle7932779 Occurrence Handle1:STN:280:ByqD3MfivFc%3D

    PubMed  CAS  Google Scholar 

  28. M.J. López-Piñón A. Insua J. Méndez (2005) ArticleTitleChromosome analysis and mapping of ribosomal genes by one- and two-color fluorescent in situ hybridization in Hinnites distortus (Bivalvia: Petinidae) J. Hered. 96 1–7

    Google Scholar 

  29. A. Louro J.P. Roche Particlede la M.J. Campos G. Román (2003) ArticleTitleHatchery rearing of the black scallop, Chlamys varia (L.) J. Shellfish Res. 22 95–99

    Google Scholar 

  30. C. Martins P.M. Galetti SuffixJr. (2001) ArticleTitleTwo rDNA arrays in Neotropical fish species: is it a general rule for fishes? Genetica 111 439–446 Occurrence Handle10.1023/A:1013799516717 Occurrence Handle11841188 Occurrence Handle1:CAS:528:DC%2BD38XhsVKqtbY%3D

    Article  PubMed  CAS  Google Scholar 

  31. Y. Matsuda K. Moriwaki V.W. Chapman Y. Hoi-Sen J. Akbarzadeh H. Suzuki (1994) ArticleTitleChromosomal mapping of mouse 5S rRNA genes by direct R-banding fluorescence in situ hybridization Cytogenet. Cell Genet. 66 246–249 Occurrence Handle8162702 Occurrence Handle1:CAS:528:DyaK2MXitVOltw%3D%3D

    PubMed  CAS  Google Scholar 

  32. D.A. Miller V.G. Dev R. Tantravahi O.J. Miller (1976a) ArticleTitleSuppression of human nucleolus organizer activity in mouse-human somatic hybrid cells Exp. Cell Res. 101 235–243 Occurrence Handle10.1016/0014-4827(76)90373-6 Occurrence Handle1:STN:280:CSiD3czktlI%3D

    Article  CAS  Google Scholar 

  33. D.A. Miller O.J. Miller V.G. Dev R. Tantravahi C.M. Croce (1976b) ArticleTitleExpression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids Proc. Natl. Acad. Sci. USA 73 4531–4535 Occurrence Handle1:STN:280:CSiD2sbhtFI%3D

    CAS  Google Scholar 

  34. B. Morton (1996) The evolutionary history of the Bivalvia J. Taylor (Eds) in Origen and Evolutionary Radiation of the Mollusca Oxford University Press Oxford 337–359

    Google Scholar 

  35. E. Pauls M.L. Pacheco M.P. Cabeza (1996) ArticleTitleCytogenetic characterization of Nodipecten nodosus embryos (Bivalvia: Pectinidae) from South Atlantic coast of Brazil Arch. Zootecnia 45 225–228

    Google Scholar 

  36. E. Pauls P.R.A.M. Affonso (2000) ArticleTitleThe karyotype of Nodipecten nodosus (Bivalvia: Pectinidae) Hydrobiologia 420 99–102 Occurrence Handle10.1023/A:1003989721626

    Article  Google Scholar 

  37. Rasotto, M., D. Altieri & D. Colombera, 1981. I cromosomi spermatocitari di 16 specie appartenenti alla classe Pelecypoda. Atti. Congr. Soc. Malac. Ital. 113–127.

  38. J. Sambrook E.F. Fritsch T. Maniatis (1989) Molecular cloning. A laboratory manual EditionNumber2 Cold Spring Harbor Laboratory Press Cold Spring Harbor, New York

    Google Scholar 

  39. C. Thiriot-Quiévreux (2002) ArticleTitleReview of the literature on bivalve cytogenetics in the last ten years Cah. Biol. Mar. 43 17–26

    Google Scholar 

  40. C. Thiriot-Quiévreux N. Ayraud (1982) ArticleTitleLes caryotypes de quelques espèces de Bivalves et de Gastéropodes marins Mar. Biol. 70 165–172

    Google Scholar 

  41. A. Torreiro M.J. Martínez-Expósito M.I. Trucco J.J. Pasantes (1999) ArticleTitleCytogenetics in Brachidontes rodriguezi d’Orb (Bivalvia, Mytilidae) Chromosome Res. 7 49–55 Occurrence Handle10.1023/A:1009275311888 Occurrence Handle10219732 Occurrence Handle1:CAS:528:DyaK1MXitlehurs%3D

    Article  PubMed  CAS  Google Scholar 

  42. R. Vitturi P. Gianguzza M.S. Colomba S. Riggio (2000) ArticleTitleCytogenetic characterization of Brachidontes pharaonis (Fisher P., 1870): karyotype, banding and fluorescent in situ hybridisation (FISH) Ophelia 52 213–220

    Google Scholar 

  43. R. Vitturi M.S. Colomba A.M. Pirrone M. Mandrioli (2002) ArticleTitlerDNA (18S-28S and 5S) colocalization and linkage between ribosomal genes and (TTAGGG)n telomeric sequence in the earthworm, Octodrilus complanatus (Annelida: Oligochaeta: Lumbricidae), revealed by single and double-color FISH J. Hered. 93 279–282 Occurrence Handle10.1093/jhered/93.4.279 Occurrence Handle12407215 Occurrence Handle1:CAS:528:DC%2BD38XosVSqsL8%3D

    Article  PubMed  CAS  Google Scholar 

  44. K.T. Wada (1978) ArticleTitleChromosome karyotypes of three bivalves the oysters, Isognomon alatus and Pinctada imbricata and the bay scallop Argopecten irradians Biol. Bull. 155 235–245

    Google Scholar 

  45. H.P. Wagner (1991) ArticleTitleReview of the European Pectinidae Vita Marina 41 1–48

    Google Scholar 

  46. T.R. Waller (1991) Evolutionary relationships among commercial scallops (Mollusca: Bivalvia: Pectinidae) S.E. Shumway (Eds) in Scallops: Biology, Ecology and Aquaculture Elsevier Science Publishers Amsterdam 1–73

    Google Scholar 

  47. T.R. Waller (1993) ArticleTitleThe evolution of “Chlamys” (Mollusca: Bivalvia: Pectinidae) in the tropical western Atlantic and eastern Pacific Amer. Malac. Bull. 10 195–249

    Google Scholar 

  48. Y. Wang X. Guo (2004a) ArticleTitleChromosomal rearrangement in Pectinidae revealed by rRNA loci and implications for bivalve evolution Biol. Bull. 207 247–256 Occurrence Handle1:CAS:528:DC%2BD2MXmsFKhsQ%3D%3D

    CAS  Google Scholar 

  49. Y. Wang X. Guo (2004b) ArticleTitleDifferences in the rDNA-bearing chromosome divide the Asian-Pacific and Atlantic species of Crassostrea (Bivalvia, Mollusca) Biol. Bull. 206 46–54 Occurrence Handle1:CAS:528:DC%2BD2cXitlahsb4%3D

    CAS  Google Scholar 

  50. D.E. Wimber D.M. Steffensen (1970) ArticleTitleLocalization of 5S RNA genes on Drosophila chromosomes by RNA-DNA hybridization Science 170 639–641 Occurrence Handle5470721 Occurrence Handle1:CAS:528:DyaE3MXhtVynsQ%3D%3D

    PubMed  CAS  Google Scholar 

  51. B. Winnepenninckx T. Backeljau R. Wachter ParticleDe (1993) ArticleTitleExtraction of high molecular weight DNA from molluscs Trends Genet. 9 407 Occurrence Handle8122306 Occurrence Handle1:CAS:528:DyaK2cXis1GisbY%3D

    PubMed  CAS  Google Scholar 

  52. E. Brand ParticleVon G. Bellolio K. Lohrmann (1990) ArticleTitleChromosome number of the Chilean scallop Argopecten purpuratus Tahoku J. Agricultural Res. 40 91–95

    Google Scholar 

  53. J.H. Xiang R.R. Desrosiers F. Dubé (1993) ArticleTitleStudies on the chromosomes of the giant scallop Placopecten magellanicus (Gmelin) and the surf clam Spisula solidissima (Dillwyn) Cytologia 58 125–132

    Google Scholar 

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Correspondence to Ana Insua.

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Insua, A., López-Piñón, M.J., Freire, R. et al. Karyotype and Chromosomal Location of 18S–28S and 5S Ribosomal DNA in the Scallops Pecten maximus and Mimachlamys varia (Bivalvia: Pectinidae). Genetica 126, 291–301 (2006). https://doi.org/10.1007/s10709-005-7408-7

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Keywords

  • FISH
  • karyotype
  • Mimachlamys varia
  • Pecten maximus
  • ribosomal DNA
  • scallops