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Genetica

, 128:159 | Cite as

Constitutive heterochromatin, 5S and 18S rDNA genes in Apareiodon sp. (Characiformes, Parodontidae) with a ZZ/ZW sex chromosome system

  • Renata da Rosa
  • Elisangela Bellafronte
  • Orlando Moreira Filho
  • Vladimir Pavan Margarido
Article

Abstract

Karyotype, sex chromosome system and cytogenetics characteristics of an unidentified species of the genus Apareiodon originating from Piquiri River (Paraná State, Brazil) were investigated using differential staining techniques (C-banding and Ag-staining) and fluorescent in situ hybridization (FISH) with 5S and 18S rDNA probes. The diploid chromosome number was 2n = 54 with 25 pairs of meta- (m) to submetacentric (sm) and 2 pairs of subtelocentric (st) chromosomes. The major ribosomal rDNA sites as revealed by Ag-staining and FISH with 18S rDNA probe were found in distal region of longer arm of st chromosome pair 26, while minor 5S sites were observed in the interstitial sites on chromosome pairs 2 (smaller cluster) and 7 (larger one). The C-positive heterochromatin had pericentromeric and telomeric distribution. The heteromorphic sex chromosome system consisted of male ZZ (pair 21) and female middle-sized m/st Z/W chromosomes. The pericentric inversion of heterochromatinized short arm of ancestral Z followed by multiplication of heterochromatin segments is hypothesized for origin of W chromosome. The observed karyotype and chromosomal markers corresponded to those found in other species of the genus.

Keywords

Chromosome banding Cytogenetics cytotaxonomy of Aparaeiodon FISH 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Renata da Rosa
    • 1
  • Elisangela Bellafronte
    • 2
  • Orlando Moreira Filho
    • 2
  • Vladimir Pavan Margarido
    • 3
  1. 1.Centro de Ciências Biológicas, Departamento de Biologia GeralUniversidade Estadual de LondrinaLondrinaBrazil
  2. 2.Departamento de Genética e EvoluçãoUniversidade Federal de São CarlosSão CarlosBrazil
  3. 3.Centro de Ciências Biológicas e da SaúdeUniversidade Estadual do Oeste do ParanáCascavelBrazil

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