Karyology of the Antarctic scallop Adamussium colbecki, with some comments on the karyological evolution of pectinids

Abstract

Karyotype, location of the nucleolar organiser region (NOR) and heterochromatin presence and composition were studied in the Antarctic scallop Adamussium colbecki Smith, 1902. The karyotype exhibits 2n = 38 chromosomes with 11 pairs of metacentrics, 5 of submetacentrics, one subtelocentric and two telocentrics. Ag–NOR, CMA3, DA/MM and NOR–FISH evidenced paracentromeric NORs on the short arm of 2nd pair chromosomes. Digestion with three restriction endonucleases followed by sequential staining with Giemsa, CMA3 and DAPI evidenced on all chromosomes centromeric heterochromatin positive for both DAPI and CMA3. In situ hybridisation analysis showed the presence of an AT-rich satellite DNA in the centromeric heterochromatin of several chromosomes. A mosaicism was detected in the germinal cell lines of one specimen, as in six of the 20 plates examined the set had 37 chromosomes with a missing pair of telocentrics and an unpaired metacentric. Comparison of the chromosome sets of all the pectinids studied to date and comparison with a phyletic tree obtained from molecular mitochondrial genes studies yielded good agreement between karyotype morphology and taxonomic classification.

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References

  1. M. Barucca E. Olmo S. Schiaparelli A. Canapa (2004) ArticleTitleMolecular phylogeny of the family Pectinidae (Mollusca: Bivalvia) based on mitochondrial 16S and 12S rRNA genes Mol. Phylogenet. Evol. 31 89–95 Occurrence Handle15019610 Occurrence Handle1:CAS:528:DC%2BD2cXhvFSks78%3D Occurrence Handle10.1016/j.ympev.2003.07.003

    PubMed  CAS  Article  Google Scholar 

  2. R. Barzotti F. Pelliccia A. Rocchi (2000) ArticleTitleSex chromosome differentiation revealed by genomic in situ hybridisation Chromosome Res. 8 459–464 Occurrence Handle11032316 Occurrence Handle1:CAS:528:DC%2BD3cXnt12itbY%3D Occurrence Handle10.1023/A:1009246520601

    PubMed  CAS  Article  Google Scholar 

  3. E. Basoa C. Alfonsi J.E. Perez H. Cequea (2000) ArticleTitleKaryotypes on the scallops Euvola ziczac and Nodipecten nodosus from the gulf of Cariaco, Sucre State, Venezuela Bol. Inst. Oceanogr. Venez. 39 49–54

    Google Scholar 

  4. A.R. Beaumont L.D. Gruffydd (1974) ArticleTitleStudies on the chromosomes of the scallop Pecten maximus (L.) and related species J. Mar. Biol. Assoc. UK 54 713–718 Occurrence Handle10.1017/S0025315400022876

    Article  Google Scholar 

  5. Beaumont, R. & E. Zouros. 1991. Genetics of scallops pp. 585–623 in Biology, Ecology and Aquaculture, edited by S.E. Shumway. Elsevier, Amsterdam.

  6. A. Canapa M. Barucca A. Marinelli E. Olmo (1999) ArticleTitleA molecular approach to the systematics of the Antarctic scallop Adamussium colbecki Ital. J. Zool. 66 379–382

    Google Scholar 

  7. A. Canapa M. Barucca A. Marinelli E. Olmo (2000a) ArticleTitleMolecular data from the 16S rRNA gene for the phylogeny of Pectinidae (Mollusca: Bivalvia) J. Mol. Evol. 50 93–97 Occurrence Handle1:CAS:528:DC%2BD3cXotFSmuw%3D%3D

    CAS  Google Scholar 

  8. A. Canapa M. Barucca A. Marinelli P. Nisi Cerioni E. Olmo T. Capriglione G. Odierna (2000b) ArticleTitleA molecular analysis of the systematics of three Antarctic bivalves Ital. J. Zool. Supp.1 127–132 Occurrence Handle10.1080/11250000009356367

    Article  Google Scholar 

  9. A. Canapa M. Barucca P. Nisi Cerioni E. Olmo (2000c) ArticleTitleA satellite DNA containing CENP-B box-like motifsis present in the Antarctic scallop Adamussium colbecki Gene 247 175–180 Occurrence Handle1:CAS:528:DC%2BD3cXisV2ksbg%3D Occurrence Handle10.1016/S0378-1119(00)00101-3

    CAS  Article  Google Scholar 

  10. D.R. Dixon I.R.B. McFadzen K. Sisley (1986) ArticleTitleHeterochromatic marker regions (nucleolar organizers) in the chromosomes of the common mussel, Mytilus edulis (Mollusca: Pelecipoda) J. Exp. Mar. Biol. Ecol. 112 1–9

    Google Scholar 

  11. G. Gajardo M. Parraguez N. Colihueque (2000) ArticleTitleKaryotype analysis and chromosome banding of the Chilean–Peruvian scallop Argopecten purpuratus (Lamarck, 1819) J. Shellfish J. Res. 21 585–590

    Google Scholar 

  12. P.W.H. Holland A.H. Hacker N.A. Williams (1991) ArticleTitleA molecular analysis of the phylogenetic affinities of Saccoglossus cambrensis Brambell and Cole (Hemichordata) Phil. Trans. R. Soc. London Series B 332 185–189 Occurrence Handle1:CAS:528:DyaK38Xht1Ghs70%3D

    CAS  Google Scholar 

  13. W.M. Howell D.A. Black (1980) ArticleTitleControlled silver staining of Nucleolus Organizer Regions with a protective colloidal developer: a 1-step method Experientia 36 1014–1015 Occurrence Handle6160049 Occurrence Handle1:STN:280:DyaL3M%2Fms12kug%3D%3D Occurrence Handle10.1007/BF01953855

    PubMed  CAS  Article  Google Scholar 

  14. H. Ieyama (1975) ArticleTitleChromosome number of three species in three families of Pteriomorpha (Bivalvia) Venus Jpn. J. Malacol. 34 26–32

    Google Scholar 

  15. A. Insua R. Freire J. Rios J. Méndez (2001) ArticleTitleThe 5S rDNA of mussels Mytilus galloprovincialis and M. edulis: sequence variation and chromosomal location Chromosome Res. 9 495–505 Occurrence Handle11592484 Occurrence Handle1:CAS:528:DC%2BD3MXnt1ektbk%3D Occurrence Handle10.1023/A:1011636714052

    PubMed  CAS  Article  Google Scholar 

  16. A. Insua M.J. Lopez-Piñón J. Méndez (1998) ArticleTitleCharacterization of Aequipecten opercularis (Bivalvia: Pectinidae) by different staining techniques and fluorescent in situ hybridisation Genes Genet. Systems 73 193–200 Occurrence Handle1:STN:280:DyaK1M%2FpslGqsA%3D%3D Occurrence Handle10.1266/ggs.73.193

    CAS  Article  Google Scholar 

  17. A. Insua M.J. Lopez-Piñón R. Freire J. Méndez (2003) ArticleTitleSequence analysis of the ribosomal DNA internal transcribed spacer region in some scallop species (Mollusca: Bivalvia: Pectinidae) Genome 46 595–604 Occurrence Handle12897868 Occurrence Handle1:CAS:528:DC%2BD3sXnsVertrw%3D Occurrence Handle10.1139/g03-045

    PubMed  CAS  Article  Google Scholar 

  18. A. Insua C. Thiriot-Quiévreux (1991) ArticleTitleThe characterization of Ostrea denselamellosa (Mollusca: Bivalvia) chromosomes, karyotype, constitutive heterochromatin and nucleolus organizer regions Aquaculture 97 317–325 Occurrence Handle10.1016/0044-8486(91)90324-Z

    Article  Google Scholar 

  19. C.M. Jacobi C. Rosenberg A.M. Vianna-Morgante (1990) ArticleTitleThe karyotype of the brown mussel Perna perna (L.) (Bivalvia: Mytilidae) Rev. Brasil Genet. 13 669–673

    Google Scholar 

  20. A. Komaru K.T. Wada (1985) ArticleTitleKaryotypes of four species in the Pectinidae (Bivalvia: Pteriomorpha) Venus Jpn. J. Malacol. 44 249–259

    Google Scholar 

  21. Lazzaretto, I. & Libertini A., 1991. Ricerche cariologiche su alcuni invertebrati marini della regione antartica: dati preliminari. Scienza e Cultura Special issue: 173–176

  22. A Leitäo P. Boundry H. McCombie C. Thiriot-Quiévreux (2001a) ArticleTitleExperimental evidence for a genetic basis to differences in aneuploidy in the pacific oyster (Crassostrea gigas) Aquat. Living Resour. 14 233–237 Occurrence Handle10.1016/S0990-7440(01)01119-6

    Article  Google Scholar 

  23. A. Leitäo P. Boundry C. Thiriot-Quiévreux (2001b) ArticleTitleEvidence of differential chromosome loss in aneuploid karyotypes of the Pacific oyster, Crassostrea gigas Genome 44 735–737 Occurrence Handle10.1139/gen-44-4-735

    Article  Google Scholar 

  24. A. Leitäo P. Boundry C. Thiriot-Quiévreux (2001c) ArticleTitleNegative correlation between aneuploidy and growth in the pacific oyster, Crassostrea gigas: ten year of evidence Aquaculture 193 39–41 Occurrence Handle10.1016/S0044-8486(00)00488-9

    Article  Google Scholar 

  25. A. Levan K. Fredga A.A. Sandberg (1964) ArticleTitleNomenclature for centromeric position on chromosomes Hereditas 52 201–220 Occurrence Handle10.1111/j.1601-5223.1964.tb01953.x

    Article  Google Scholar 

  26. A. Libertini M. Panozzo R. Zenere (1993) ArticleTitleChromosome number and genome size in the Antarctic whelk, Neobuccinum eatoni (Smith) (Prosobranchia, Neogastropoda) Chromosome Information Service 55 5–6

    Google Scholar 

  27. M.J. López-Piñón A. Insua J. Méndez (2005) ArticleTitleChromosome analysis and mapping of ribosomal genes by one- and two-color fluorescent in situ hybridization in Hinnites distortus (Bivalvia: Pectinidae) J. Hered. 96 52–58 Occurrence Handle15598716 Occurrence Handle10.1093/jhered/esi001 Occurrence Handle1:CAS:528:DC%2BD2MXkt1aisA%3D%3D

    PubMed  Article  CAS  Google Scholar 

  28. A. Martinéz-Lage A. Gonzalez-Tizòn J. Méndez (1994) ArticleTitleCharacterization of different chromatin types in Mytilus galloprovincialis L. after C-banding, fluorochrome and restriction endonuclease treatments Heredity 72 242–249

    Google Scholar 

  29. M. Matsumoto I. Hayami (2000) ArticleTitlePhylogenetic analysis of the family Pectinidae (Bivalvia) based on mitochondrial cytochrome C oxidase subunit I J. Moll. Stud. 66 477–488 Occurrence Handle10.1093/mollus/66.4.477

    Article  Google Scholar 

  30. R. Mezzanotte U. Bianchi R. Vanni L. Ferrucci (1983) ArticleTitleChromatin organization and restriction nuclease activity on human metaphase chromosomes Cytogenet. Cell Genet. 36 562–566 Occurrence Handle6315311 Occurrence Handle1:STN:280:DyaL2c%2Fls1OhtA%3D%3D

    PubMed  CAS  Google Scholar 

  31. A. Morescalchi (1975) ArticleTitleChromosome evolution in the caudate Amphibia Evol. Biol. 8 339–387 Occurrence Handle1:CAS:528:DyaE28XktVaksbo%3D

    CAS  Google Scholar 

  32. E. Pauls P.R. Affonso (2000) ArticleTitleThe karyotype of Nodipecten nodosus (Bivalvia: Pectinidae) Hydrobiologia 420 99–102 Occurrence Handle10.1023/A:1003989721626

    Article  Google Scholar 

  33. O. Picariello L. Feliciello I. Bellinello G. Chinali (2002) ArticleTitleS1 satellite DNA as a taxonomic marker in brown frogs: molecular evidence that Rana graeca graeca and Rana graeca italica are different species Genome 45 63–72 Occurrence Handle11908670 Occurrence Handle1:CAS:528:DC%2BD38XhslarsLs%3D Occurrence Handle10.1139/g01-125

    PubMed  CAS  Article  Google Scholar 

  34. M. Rasotto D. Altieri D. Colombera (1981) ArticleTitleI cromosomi spermatocitari di 16 specie appartenenti alla classe Pelecypoda Atti Congr. Soc. Malacol. Ital. 1198 113–127

    Google Scholar 

  35. E. Sahar S.A. Latt (1980) ArticleTitleEnergy transfer and binding competition between dyes used to enhance staining differentiation in metaphase chromosomes Chromosoma 79 1–28 Occurrence Handle6156806 Occurrence Handle1:CAS:528:DyaL3cXlsVGkt7k%3D Occurrence Handle10.1007/BF00328469

    PubMed  CAS  Article  Google Scholar 

  36. M. Schmid (1978) ArticleTitleChromosome banding in Amphibia. II. Constitutive heterochromatin and nucleolar organizer regions in Ranidae, Microhylidae and Rhacophoridae Chromosoma 77 83–103 Occurrence Handle10.1007/BF00292043

    Article  Google Scholar 

  37. M. Schmid (1982) ArticleTitleChromosome banding in Amphibia. VII. Analysis of the structure and variability of NORs in Anura Chromosoma 87 327–344 Occurrence Handle10.1007/BF00327634

    Article  Google Scholar 

  38. C. Thiriot-Quiévreux (2002) ArticleTitleReview of the literature on bivalve cytogenetics in the last ten years Cah. Biol. Mar. 43 17–26

    Google Scholar 

  39. E. Brand ParticleVon G. Bellolio K. Lohrmann (1990) ArticleTitleChromosome number of the Chilean scallop Argopecten purpuratus Tohoku J. Agric. Res. 40 91–95

    Google Scholar 

  40. T.R. Waller (1993) ArticleTitleThe evolution of “Chlamys” (Mollusca: Bivalvia: Pectinidae) in the tropical western Atlantic and eastern Pacific Am. Malacol. Bull. 10 195–249

    Google Scholar 

  41. Y. Wang X. Guo (2004) ArticleTitleChromosomal rearrangement in Pectinidae revealed by rRNA loci and implications for bivalve evolution Biol. Bull. 207 247–256 Occurrence Handle15616355 Occurrence Handle1:CAS:528:DC%2BD2MXmsFKhsQ%3D%3D

    PubMed  CAS  Google Scholar 

  42. J.H. Xiang R.R. Desrosiers F. Dube (1993) ArticleTitleStudies on the chromosomes of the giant scallop Placopecten magellanicus (Gmelin) and the surf clam Spisula solidissima (Dillwyn) Cytologia 58 125–132

    Google Scholar 

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Odierna, G., Aprea, G., Barucca, M. et al. Karyology of the Antarctic scallop Adamussium colbecki, with some comments on the karyological evolution of pectinids. Genetica 127, 341 (2006). https://doi.org/10.1007/s10709-005-5366-8

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Keywords

  • Adamussium colbecki
  • chromosomes
  • intraindividual mosaicism
  • karyotype
  • rearrangements