Skip to main content
SpringerLink
Log in

Polymorphic Chromosomal Specificity of Centromere Satellite Families in Arabidopsis halleri ssp. gemmifera

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

The chromosomal localizations of repetitive DNA clusters (ribosomal DNA and centromere satellites) were analyzed by fluorescent in situ hybridization in five strains of Arabidopsis halleri ssp. gemmifera. All five A. gemmifera strains have three chromosome pairs with 45S (5.8S-16S-26S) rDNA loci, and one pair with both 5S and 45S rDNA loci. These localizations are different from that of A. thaliana. Very unusually, there are three families of centromeric satellite DNAs (pAa, pAge1, and pAge2), and they showed polymorphism among the five strains studied. Overall, we found four different centromere satellite compositions. A plant from Fumuro was heterozygous for the chromosome specificities of centromere satellite families, possibly due to a reciprocal translocation involving centromere regions. Changes of centromeric satellite repeats appear to be rapid and frequent events in the history of A. gemmifera, and seem to occur by exchanging clusters as units.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

FISH:

fluorescent in situ hybridization

RDNA:

ribosomal DNA

References

  • R. Abranches A.F. Beven L. Aragon-Alcaide P.J. Shaw (1998) ArticleTitleTranscription sites are not correlated with chromosome territories in wheat nuclei J. Cell Biol. 143 5–12 Occurrence Handle10.1083/jcb.143.1.5 Occurrence Handle9763416 Occurrence Handle1:CAS:528:DyaK1cXmsFalsbg%3D

    Article  PubMed  CAS  Google Scholar 

  • S.J. Armstrong F.C.H. Jones G.H. Jones (2001) ArticleTitleNucleolus-associated telomere clustering and pairing precede meiotic chromosome synapsis in Arabidopsis thaliana J. Cell Sci. 114 4207–4217 Occurrence Handle11739653 Occurrence Handle1:CAS:528:DC%2BD3MXptlKrtL0%3D

    PubMed  CAS  Google Scholar 

  • C. Cloix S. Tutois Y. Yukawa O. Mathieu C. Cuvillier M.C. Espagnol G. Picard S. Tourmente (2001) ArticleTitleAnalysis of the 5S RNA pool in Arabidopsis thaliana: RNAs are heterogeneous and only two of the genomic 5S loci produce mature 5S RNA Genome Res. 12 132–144

    Google Scholar 

  • G.P. Copenhaver D. Preuss (1999) ArticleTitleCentromere in the genomic era: unraveling paradoxes Curr. Opin. Plant Biol. 2 104–108 Occurrence Handle10.1016/S1369-5266(99)80021-1 Occurrence Handle10322201 Occurrence Handle1:CAS:528:DyaK1MXivVGlsL4%3D

    Article  PubMed  CAS  Google Scholar 

  • G.P. Copenhaver K. Nickel T. Kuromori M.I. Benito S. Kaul X. Lin M. Bevan G. Murphy B. Harris L.D. Parnell W.R. Mccombie R.A. Martienssen M. Marra D. Preuss (1999) ArticleTitleGenetic definition and sequence analysis of Arabidopsis centromere Science 286 2468–2474 Occurrence Handle10.1126/science.286.5449.2468 Occurrence Handle10617454 Occurrence Handle1:CAS:528:DC%2BD3cXmvVak

    Article  PubMed  CAS  Google Scholar 

  • A.K. Csink S. Henikoff (1998) ArticleTitleSomething from nothing: the evolution and utility of satellite repeat Trends Genet. 14 200–204 Occurrence Handle10.1016/S0168-9525(98)01444-9 Occurrence Handle9613205 Occurrence Handle1:CAS:528:DyaK1cXjt1agurY%3D

    Article  PubMed  CAS  Google Scholar 

  • F. Dong J. Jiang (1998) ArticleTitleNon-Rabl patterns of centromere and telomere distribution in the interphase nuclei of plant cells Chromosome Res. 6 551–558 Occurrence Handle10.1023/A:1009280425125 Occurrence Handle9886774 Occurrence Handle1:CAS:528:DyaK1MXisVygsQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • A.E. Franklin W.Z. Cande (1999) ArticleTitleNuclear organization and chromosome segregation Plant Cell 11 523–534 Occurrence Handle10213775 Occurrence Handle1:CAS:528:DyaK1MXjtFSrtbs%3D

    PubMed  CAS  Google Scholar 

  • P. Fransz S. Armstrong C. Alonso-Blanco T.C. Fischer R.A. Torres-Ruiz D.C. Ward (1998) ArticleTitleCytogenetics for the model system Arabidopsis thaliana Plant J. 136 867–876

    Google Scholar 

  • P. Fransz J.H. Jong Particlede M. Lysak M.R. Castiglione I. Schubert (2002) ArticleTitleInterphase chromosomes in Arabidopsis are organized as well defined chromocenters from which euchromatin loops emanate Proc. Natl. Acad. Sci. USA 99 14584–14589 Occurrence Handle10.1073/pnas.212325299 Occurrence Handle12384572 Occurrence Handle1:CAS:528:DC%2BD38XosF2hsb0%3D

    Article  PubMed  CAS  Google Scholar 

  • S. Henikoff K. Ahmad H.S. Malik (2001) ArticleTitleThe centromere paradox: stable inheritance with rapidly evolving DNA Science 293 1098–1102 Occurrence Handle10.1126/science.1062939 Occurrence Handle11498581 Occurrence Handle1:CAS:528:DC%2BD3MXmtVWku7o%3D

    Article  PubMed  CAS  Google Scholar 

  • J.S. Heslop-Harrison (2000) ArticleTitleComparative genome organization in plants: From sequence and marker to chromatin and chromosomes Plant Cell 12 617–635 Occurrence Handle10.1105/tpc.12.5.617 Occurrence Handle10810139 Occurrence Handle1:CAS:528:DC%2BD3cXjvFyhur8%3D

    Article  PubMed  CAS  Google Scholar 

  • J.S. Heslop-Harrison M. Murata Y. Ogura T. Schwarzacher F. Motoyoshi (1999) ArticleTitlePolymorphism and genomic organization of repetitive DNA from centromeric regions of Arabidopsis thaliana Plant Cell 11 31–42 Occurrence Handle10.1105/tpc.11.1.31 Occurrence Handle9878630 Occurrence Handle1:CAS:528:DyaK1MXhtVGrsLg%3D

    Article  PubMed  CAS  Google Scholar 

  • J.S. Heslop-Harrison A. Brandes T. Schwarzacher (2003) ArticleTitleTandemly repeated DNA sequences and centromeric chromosomal regions of Arabidopsis species Chromosome Res. 11 241–253 Occurrence Handle10.1023/A:1022998709969 Occurrence Handle12769291 Occurrence Handle1:CAS:528:DC%2BD3sXit1Chsr8%3D

    Article  PubMed  CAS  Google Scholar 

  • A. Kamm I. Glasso T. Schmidt J.S. Heslop-Harrison (1995) ArticleTitleAnalysis of a repetitive DNA family from Arabidopsis arenosa and relationship between Arabidopsis species Plant Mol. Biol. 27 853–862 Occurrence Handle10.1007/BF00037014 Occurrence Handle7766876 Occurrence Handle1:CAS:528:DyaK2MXmtFSgs7o%3D

    Article  PubMed  CAS  Google Scholar 

  • A. Kawabe N.T. Miyashita (2002) ArticleTitleDNA variation in the acidic chitinase locus (ChiA) region in Arabis gemmifera and among its related species Genes Genet. Syst. 77 167–175 Occurrence Handle12207038 Occurrence Handle1:CAS:528:DC%2BD38XnvVSku74%3D

    PubMed  CAS  Google Scholar 

  • A. Kawabe N.T. Miyashita (2003) ArticleTitleDNA polymorphism in active gene and pseudogene of the cytosolic phosphoglucose isomerase (PgiC) loci in Arabidopsis halleri ssp. gemmifera Mol. Biol. Evol. 20 1043–1050 Occurrence Handle12716977 Occurrence Handle1:CAS:528:DC%2BD3sXltlenu7w%3D

    PubMed  CAS  Google Scholar 

  • A. Kawabe S. Nasuda (2005) ArticleTitleStructure and genomic organization of centromeric repeats in Arabidopsis species Mol. Gen. Genomics 272 593–602 Occurrence Handle10.1007/s00438-004-1081-x Occurrence Handle1:CAS:528:DC%2BD2MXhsVGksL4%3D

    Article  CAS  Google Scholar 

  • M.A. Koch B. Haubold T. Mitchell-Olds (2000) ArticleTitleComparative evolutionary analysis of chalcone synthase and alcohol dehydrogenase loci in Arabidopsis, Arabis, and related genera (Brassicaceae) Mol. Biol. Evol. 17 1483–1498 Occurrence Handle11018155 Occurrence Handle1:CAS:528:DC%2BD3cXnt1Cnsb0%3D

    PubMed  CAS  Google Scholar 

  • J. Maluszynska J.S. Heslop-Harrison (1991) ArticleTitleLocalization of tandemly repeated DNA sequences in Arabidopsis thaliana Plant J. 1 159–166

    Google Scholar 

  • J. Maluszynska J.S. Heslop-Harrison (1993) ArticleTitleMolecular cytogenetics of the genus Arabidopsis: in situ localization of rDNA sites, chromosome numbers and diversity in centromeric heterochromatin Ann. Bot. 71 479–484 Occurrence Handle10.1006/anbo.1993.1063 Occurrence Handle1:CAS:528:DyaK3sXmsVGkurg%3D

    Article  CAS  Google Scholar 

  • J. Martinez-Zapater M.A. Estelle C.R. Somerville (1986) ArticleTitleA highly repeated DNA sequence in Arabidopsis thaliana Mol. Gen. Genet. 204 417–423 Occurrence Handle10.1007/BF00331018 Occurrence Handle1:CAS:528:DyaL28XlvVGiurk%3D

    Article  CAS  Google Scholar 

  • N.T. Miyashita A. Kawabe H. Innan R. Terauchi (1998) ArticleTitleIntra- and interspecific DNA variation and codon bias of alcohol dehydrogenase (Adh) locus in Arabis and Arabidopsis species Mol. Biol. Evol. 15 1420–1429 Occurrence Handle12572606 Occurrence Handle1:CAS:528:DyaK1cXntlOgsro%3D

    PubMed  CAS  Google Scholar 

  • M. Murata Y. Ogura F. Motoyoshi (1994) ArticleTitleCentromeric repetitive sequence in Arabidopsis thaliana Jpn. J. Genet. 69 361–370 Occurrence Handle10.1266/jjg.69.361 Occurrence Handle7545957 Occurrence Handle1:CAS:528:DyaK2MXitFWjtLo%3D

    Article  PubMed  CAS  Google Scholar 

  • M. Murata J.S. Heslop-Harrison F. Motoyoshi (1997) ArticleTitlePhysical mapping of the 5S ribosomal RNA genes in Arabidopsis thaliana by multi-color fluorescence in situ hybridization with cosmid clones Plant J. 12 31–37 Occurrence Handle10.1046/j.1365-313X.1997.12010031.x Occurrence Handle9263450 Occurrence Handle1:CAS:528:DyaK2sXlsFCrt70%3D

    Article  PubMed  CAS  Google Scholar 

  • O. Pontes N. Neves M. Silva M.S. Lewis A. Madlung L. Comai W. Viegas C.S. Pikaard (2004) ArticleTitleChromosomal locus rearrangements are a rapid response to formation of the allotetraploid Arabidopsis suecica genome Proc. Natl. Acad. Sci. USA 101 18240–18245 Occurrence Handle10.1073/pnas.0407258102 Occurrence Handle15604143 Occurrence Handle1:CAS:528:DC%2BD2MXjsl2kuw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • C. Rabl (1885) ArticleTitleÜber Zelltheilung Morphol. Jahrb. 10 214–330

    Google Scholar 

  • E.J. Richards R.K. Dawe (1998) ArticleTitlePlant centromere: structure and control Curr. Opin. Plant Biol. 1 130–135 Occurrence Handle10.1016/S1369-5266(98)80014-9 Occurrence Handle10066578 Occurrence Handle1:STN:280:DyaK1M7mslOgtQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • E.K. Round S.K. Flowers E.J. Richards (1997) ArticleTitleArabidopsis thaliana centromere regions: genetic map position and repetitive DNA structure Genome Res. 7 1045–1053 Occurrence Handle9371740 Occurrence Handle1:CAS:528:DyaK2sXns1Ojs7c%3D

    PubMed  CAS  Google Scholar 

  • InstitutionalAuthorNameThe Arabidopsis Genome Initiative (2000) ArticleTitleAnalysis of genome sequence of the flowering plant Arabidopsis thaliana Nature 408 796–815 Occurrence Handle10.1038/35048692

    Article  Google Scholar 

  • P.E. Warburton T. Haaf J. Gosden D. Lawson H.F. Willard (1996) ArticleTitleCharacterization of a chromosome-specific chimpanzee alpha satellite subset: evolutionary relationship to subsets on human chromosomes Genomics 33 220–228 Occurrence Handle10.1006/geno.1996.0187 Occurrence Handle8660971 Occurrence Handle1:CAS:528:DyaK28Xis1ensb8%3D

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, 606-8502, Sakyo-ku Kyoto, Japan

    Akira Kawabe & Shuhei Nasuda

  2. CREST, Japan Science and Technology Corporation, 101-0062, Tokyo, Japan

    Akira Kawabe & Shuhei Nasuda

  3. Institute of Evolutionary Biology, University of Edinburgh, Ashworth Laboratories King’s Buildings, West Mains Road, EH9 3JT, Edinburgh, UK

    Akira Kawabe

Authors
  1. Akira Kawabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shuhei Nasuda
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Akira Kawabe.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kawabe, A., Nasuda, S. Polymorphic Chromosomal Specificity of Centromere Satellite Families in Arabidopsis halleri ssp. gemmifera. Genetica 126, 335–342 (2006). https://doi.org/10.1007/s10709-005-0718-y

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10709-005-0718-y

Keywords

Search

Navigation