Abstract
Karyotype and cytogenetic markers of Oligosarcus hepsetus from two Brazilian locations in the Paraíba do Sul River Basin (Brazil) were investigated using differential staining techniques (C-banding, silver (Ag)- and chromomycin A3 (CMA3)-staining) and fluorescent in situ hybridization (FISH) using 18 S rDNA and 5 S rDNA probes. The diploid chromosome number was invariably 2n = 50 with 3 pairs of metacentric, 5 pairs of submetacentric, 8 pairs of subtelocentric and 9 pairs of acrocentric chromosomes. No heteromorphic sex chromosomes were observed. The nucleolar organizer regions (NORs) were detected in the short arms of the largest acrocentric pair using Ag-, CMA3- stainings and FISH with 18 S rDNA probe, the latter showing also positive labeling in the short arms of a small acrocentric pair, not visualized by the former methods. FISH with 5 S rDNA probe showed positive labeling in the two chromosome pairs. While the CMA3-staining exhibited GC-rich heterochromatin segments in two pairs of chromosomes, including those coincided with Ag-NORs, the DAPI staining did not reveal any signal, indicating the absence of AT-rich heterochromatin. FISH with an As-51 satellite DNA probe derived from the closely related Astyanax scabripinnis did not reveal any positive signal, demonstrating the absence of this class of DNA in the genome of the specimens under study.
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References
Abel, L.D.S., 2001. A variabilidade do complexo de espécies scabripinnis (Pisces, Characidae) como estratégia adaptativa. Estudo da diversidade cariotípica do grupo com ênfase em populações da bacia do rio São Francisco. Master’s Thesis, Universidade Federal de São Carlos, São Carlos - SP, Brazil
L.F. Almeida-Toledo C. Ozouf-Costaz F. Foresti C. Bonillo F. Porto-Foresti M.F.Z. Daniel-Silva (2002) ArticleTitleConservation of the 5 S-bearing chromosome pair and co-localization with major rDNA clusters in five species of Astyanax (Pisces, Characidae) Cytogenet. Genome Res 97 229–233 Occurrence Handle10.1159/000066609 Occurrence Handle1:CAS:528:DC%2BD38XovVeisrw%3D Occurrence Handle12438717
R.F. Artoni L.A.C. Bertollo (1999) ArticleTitleNature and distribution of constitutive heterochromatin in fishes, genus Hypostomus (Loricariidae) Genetica 106 209–214 Occurrence Handle10.1023/A:1003957719178 Occurrence Handle1:STN:280:DC%2BD3czpsFOrsg%3D%3D Occurrence Handle10897794
L.A.C. Bertollo C.S. Takahashi O. Moreira-Filho (1978) ArticleTitleCytotaxonomic considerations on Hoplias lacerdae (Pisces, Erythrinidae) Rev. Bras. Genet 1 IssueID2 103–120
P.A. Buckup (1993) ArticleTitleThe monophyly of the Characidiinae, a Neotropical group of Characiform fishes (Teleostei: Ostariophysi) Zool. J. Linn. Soc 108 225–245 Occurrence Handle10.1006/zjls.1993.1024
Cunha, E.B., J.L. Pegoraro & V.P. Margarido, 2001. Análise citogenética comparativa de duas espécies de Oligosarcus (Pisces, Characidae, Acestrorhynchinae). IN: 47th Congresso Nacional de Genética, Águas de Lindóia – SP. CD-ROM
Daniel-Silva, M.F.Z., 2001. Análises citogenéticas comparativas em Characidae (Pisces, Characiformes). Doctoral Thesis, Universidade de São Paulo, São Paulo – SP, Brazil
Falcão, J.N., 1983. Estudos citogenéticos em Acestrorhynchinae e Cynopotaminae (Pisces, Characidae). Master’s Thesis, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto – SP, Brazil
J.N. Falcão L.A.C. Bertollo (1985) ArticleTitleChromosome caracterization in Acestrorhynchinae and Cynopotaminae (Pisces: Characidae) J. Fish Biol 27 603–610
D.A.M. Ferro D.M. Neo Moreira-Filho O. L.A.C. Bertollo (2001) ArticleTitleNucleolar organizing regions, 18 S and 5 S rDNA in Astyanax scabripinnis (Pisces, Characidae): populations distribution and functional diversity Genetica 110 55–62
T. Hatanaka P.M. Galetti SuffixJr. (2004) ArticleTitleMapping of the 18 S and 5 S ribosomal RNA genes in the fish Prochilodus argenteus Agassiz, 1829 (Characiformes, Prochilodontidae) Genetica 122 239–244 Occurrence Handle10.1007/s10709-004-2039-y Occurrence Handle1:CAS:528:DC%2BD2cXpsFagt74%3D Occurrence Handle15609546
Hattori, R.S., C.E. Lopes & L.F. Almeida-Toledo, 2002. Estudos citogenéticos comparativos em três espécies do gênero Oligosarcus (Pisces, Characiformes). IN: 48th Congresso Nacional de Genética, Águas de Lindóia – SP. CD-ROM
W.M. Howell D.A. Black (1980) ArticleTitleControlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method Experientia 36 1014–1015 Occurrence Handle1:STN:280:Bi6D28npslw%3D Occurrence Handle6160049
Kavalco, K.F., 2003. Contribuição citogenética à análise da biodiversidade da ictiofauna das nascentes do rio Paraitinga. Master’s Thesis, Universidade Federal de São Carlos, São Carlos – SP, Brazil
K.F. Kavalco O. Moreira-Filho (2003) ArticleTitleCytogenetical analyses in four species of the genus A styanax (Pisces, Characidae) from Paraíba do Sul river basin Caryologia 56 453–461
K.F. Kavalco R. Pazza L.A.C. Bertollo O. Moreira-Filho (2004) ArticleTitleGene mapping of 5 S rDNA in eight fish species from the Paraíba do Sul river basin, Brazil Cytogenet. Genome Res 106 107–110 Occurrence Handle10.1159/000078567 Occurrence Handle1:CAS:528:DC%2BD2cXlt1Slt7Y%3D Occurrence Handle15218249
A. Levan K. Fredga A.A. Sandberg (1964) ArticleTitleNomenclature for centromeric position on chromosomes Hereditas 52 201–220
Lima, R.S., 1997. Ictiofauna do alto curso do Rio Paraíba do Sul. Master’s Thesis, Universidade de São Paulo, São Paulo – SP, Brazil
Lucena, C.A.S., 1993. Estudo filogenético da família Characidae com uma discussão dos grupos naturais propostos (Teleostei, Ostariophysi, Characiformes). Doctoral Thesis, Universidade de São Paulo, São Paulo – SP, Brazil
C.A.S. Lucena N.A. Menezes (1998) A phylogenetic analysis of Roestes Günther and Gilbertolus Eigenmann, with a hypothesis on the relationships of the Cynodontidae and Acestrorhynchidae (Teleostei: Ostariophysi: Characiformes) L.R. Malabarba R.E. Reis R.P. Vari Z.M.S. Lucena C.A. Lucena (Eds) Phylogeny and Classification of Neotropical Fishes EDIPUCRS Porto Alegre, RS, Brazil 261–278
V.P. Margarido P.M. Galetti SuffixJr. (1999) ArticleTitleHeterochromatin patterns and karyotype relationships within and between the genera Brycon and Salminus (Pisces, Characidae) Genet. Mol. Biol 22 357–61 Occurrence Handle10.1590/S1415-47571999000300012
Martinez, E.R.M. & H.F. Julio Jr., 2001. Análise citogenética de Oligosarcus cf. paranensis (Characidae, Acestrorhynchinae) do ribeirão Keller, bacia do Paraná. IN: 47th Congresso Nacional de Genética, Águas de Lindóia – SP. CD-ROM
C. Martins P.M. Galleti SuffixJr. (1999) ArticleTitleChromosomal localization of 5 S rDNA genes in Leporinus fish (Anostomidae, Characiformes) Chromosome Res 7 363–367 Occurrence Handle10.1023/A:1009216030316 Occurrence Handle1:CAS:528:DyaK1MXlvFyktbk%3D Occurrence Handle10515211
C. Martins P.M. Galetti SuffixJr (2000) ArticleTitleConservative distribution of 5 S rDNa loci in Schizodon (Pisces, Anostomidae) chromosomes Chromosome Res 8 353–355 Occurrence Handle10.1023/A:1009243815280 Occurrence Handle1:CAS:528:DC%2BD3cXltlShur8%3D Occurrence Handle10919726
C. Martins P.M. Galetti SuffixJr. (2001) ArticleTitleOrganization of 5 S rDNA in species of the fish Leporinus: two different genomic locations are characterized by distinct nontranscribed spacers Genome 44 903–910 Occurrence Handle10.1139/gen-44-5-903 Occurrence Handle1:CAS:528:DC%2BD3MXos12ls7k%3D Occurrence Handle11681615
N.A. Menezes (1987) ArticleTitleTrês espécies novas de Oligosarcus Günther, 1864 e redefinição taxonômica das demais espécies do gênero (Osteichthyes, Teleostei, Characidae) Bol. Zool. Univ. S. Paulo 11 1–39
N.A. Menezes J. Géry (1983) ArticleTitleSeven new Acestrothynchin characid species (Osteichthyes, Ostariophysi, Characiformes) with comments on the systematics of the group Revue Suisse Zool 90 563–592
C.A. Mestriner P.M. Galetti SuffixJr. S.R. Valentini I.R.G. Ruiz L.D.S. Abel O. Moreira-Filho J.P.M. Camacho (2000) ArticleTitleStructural and functional evidence that B chromosome in the characid fish Astyanax scabripinnis is an isochromosome Heredity 85 1–9 Occurrence Handle10.1046/j.1365-2540.2000.00702.x Occurrence Handle1:CAS:528:DC%2BD3cXmsFOgsbs%3D Occurrence Handle10971685
S. Morelli L.A.C. Bertollo F. Foresti O. Moreira-Filho S.A. Toledo-Filho (1983) ArticleTitleCytogenetic considerations on the genus Astyanax (Pisces, Characidae). I. Karyotypic variability Caryologia 36 235–244
O. Moreira-Filho L.A.C. Bertollo (1991) ArticleTitleAstyanax scabripinnis (Pisces, Characidae): a species complex Brazil. J. Genet 14 331–357
Mortati, A.F. & A.L. Dias, 2001. Dados cariotípicos de duas populações de Oligosarcus (Pisces, Characidae), coletados na bacia do rio Tibagi – PR. IN: 47° Congresso Nacional de Genética, Águas de Lindóia – SP. CD-ROM
C. Oliveira L.F.A. Toledo F. Foresti H.A. Britski S.A. Toledo Filho (1988) ArticleTitleChromosome formulae of neotropical freshwater fishes Genet. Mol. Biol 11 577–624
D. Pinkel T. Straume J.W. Gray (1986) ArticleTitleCytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization Proc. Natl. Acad. Sci. USA 83 2934–2938 Occurrence Handle1:CAS:528:DyaL28XktlynsbY%3D Occurrence Handle3458254
A.L.B.S. Portela P.M. Galetti SuffixJr. L.A.C. Bertollo (1988) ArticleTitleConsiderations on the chromosome evolution of Tetragonopterinae (Pisces, Characidae) Brazil. J. Genet 11 307–316
M.A. Salvador V.P. Margarido (1999) ArticleTitleAnálise citogenética em Oligosarcus sp. (Pisces, Characidae, Acestrorhynchinae) coletados no rio Sapucaí – Bacia do Rio Piquiri, Região de Cascavel, PR Genet. Mol. Biol 22 IssueIDSuppl 91
J.J. Scheel (1973) Fish chromosomes and their evolution. Internal Report of Denmarks Akvarium Charlottenlund Denmark 22
M. Schmid (1980) ArticleTitleChromosome banding in Amphibia. IV. Differentiation of GC- and AT-rich chromosome region in Anura Chromosoma 77 83–103 Occurrence Handle10.1007/BF00292043 Occurrence Handle1:CAS:528:DyaL3cXhvFCrtr4%3D Occurrence Handle7371452
D. Schweizer (1980) ArticleTitleSimultaneous fluorescent staining of R-bands and specific heterochromatic regions (DA-DAPI bands) in human chromosomes Cytogenet. Cell Genet 27 190–193 Occurrence Handle1:STN:280:Bi%2BB2crjtV0%3D Occurrence Handle6156801
A.T. Sumner (1972) ArticleTitleA simple technique for demonstrating centromeric heterocromatin Exp. Cell Res 75 304–306 Occurrence Handle10.1016/0014-4827(72)90558-7 Occurrence Handle1:STN:280:CSyD2cjltFI%3D Occurrence Handle4117921
L.C.S. Veiga M.M.R. Vieira F. Foresti (1998) ArticleTitleEstudos citogenéticos em Oligosarcus jenynsii (Pisces, Characidae) Genet. Mol. Biol 21 IssueIDSuppl. 64
A.P. Wasko C. Martins J.M. Wright P.M. Galetti SuffixJr. (2001) ArticleTitleMolecular organization of 5 S rDNA in fishes of the genus Brycon Genome 44 893–902 Occurrence Handle10.1139/gen-44-5-893 Occurrence Handle1:CAS:528:DC%2BD3MXos12ls7g%3D Occurrence Handle11681614
S.H. Weitzman L.R. Malabarba (1998) Perspectives about the phylogeny and classification of the Characidae (Teleostei: Characiformes) L.R. Malabarba R.E. Reis R.P. Vari Z.M.S. Lucena C.A. Lucena (Eds) Phylogeny and Classification of Neotropical Fishes EDIPUCRS Porto Alegre RS Brazil 161–170
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Kavalco, K.F., Pazza, R., Bertollo, L.A.C. et al. Molecular cytogenetics of Oligosarcus hepsetus (Teleostei, Characiformes) from two Brazilian locations. Genetica 124, 85–91 (2005). https://doi.org/10.1007/s10709-005-0176-6
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DOI: https://doi.org/10.1007/s10709-005-0176-6