, 123:227 | Cite as

Chromosomal diversification of reef fishes from genus Centropyge(Perciformes, Pomacanthidae)

  • Paulo Roberto Antunes de Mello Affonso
  • Pedro Manoel GalettiJr


The genus Centropyge is remarkable for species richness, composing a highly specialized fish group amongst members from family Pomacanthidae. However, cytogenetical reports are nearly absent in these animals. New data are provided from karyotypical studies carried out on Centropyge aurantonotus from the Brazilian coast of the Atlantic Ocean and C. ferrugatus from the Philippines Sea of the Indo-Pacific Ocean. Both species present 2n=48 but karyotypes are differentiated by fundamental number. C. aurantonotus has a great number of biarmed chromosomes (4 m + 14 sm+16 st+4 a), while C. ferrugatus presents only acrocentric chromosomes. Single nucleolar organizer regions (NORs) are located at interstitial position of an acrocentric pair in C. ferrugatus and on short arms of a subtelocentric pair in C. aurantonotus, as confirmed by fluorescent in situ hybridization (FISH) with 18S rDNA probes. Heterochromatin is distributed over NOR and centromeric regions in both species, but additional GC-rich heterochromatic blocks on short arms of up to eight chromosomal pairs can be detected in C. aurantonotus. 5S rDNA segments were located interstitially on two chromosomal pairs in C. ferrugatus and on nine pairs in C. aurantonotus, mostly equivalent to heterochromatic blocks on short arms of biarmed chromosomes. C. ferrugatus can be considered a species in which basal chromosomal features proposed for modern Teleosteans were conserved. The derived karyotype pattern of C. aurantonotus seems to be determined by pericentric inversions and heterochromatin addition which probably determined the notorious dispersion of 5S rRNA (pseudo)genes. It is demonstrated that, even within a group generally characterized by cytogenetical homogeneity as the family Pomacanthidae, diversified karyotypes can be found.


biogeography dispersal FISH heterochromatin NOR pericentric inversion Pomacanthidae 5S 18S 


  1. Affonso, P.R.A.M., Guedes, W., Pauls, E., Galetti, P.M.,Jr 2001Cytogenetic analysis of coral reef fishes from Brazil (families Pomacanthidae and Chaetodontidae)Cytologia66379384Google Scholar
  2. Affonso, P.R.A.M., Guedes, W., Pauls, E., Galetti, P.M.,Jr 2002Close karyotypical relationship between two species of marine angelfishes from South Atlantic: Pomacanthus arcuatus and P. paru (Perciformes, Pomacanthidae)Caryologia55323329Google Scholar
  3. Arai, R., Inoue, M. 1975Chromosomes of nine species of Chaetodontidae and one species of Scorpidae from JapanBull. Natl. Sci. Museum Tokyo, Ser. A (Zoology)1217224Google Scholar
  4. Bauer, J.A., Bauer, S.E. 1981Reproductive biology of pigmy angelfishes of the genus Centropyge (Pomacanthidae)Bull. Mar. Sci.31495513Google Scholar
  5. Chung, K.C., Woo, N.Y.S. 1998Phylogenetic relationships of the Pomacanthidae (Pisces: Teleostei) inferred from allozyme variationJ. Zool.246215231Google Scholar
  6. Deiana A., M., Cau, A., Salvadori, S., Coluccia, E., Camas, R., Milia, A., Tagliavini, J. 2000Major and 5S ribosomal sequences of the largemouth bass Micropterus salmoides (Perciformes, Centrarchidae) are localized in GC-rich regions of the genomeChrom. Res.8213218PubMedGoogle Scholar
  7. Foresti, F., Oliveira, C., Almeida-Toledo, L.F. 1993A method for chromosome preparations from large specimens of fish using in vitro short treatment with colchicineExperientia49810813Google Scholar
  8. Galetti, P.M.,Jr, Aguilar, C.T., Molina, W. 2000An overview of marine fish cytogeneticsHydrobiologia4205562Google Scholar
  9. Goodbred, C.O., Graves, J.E. 1996Genetic relationships among geographically isolated populations of bluefish (Pomatomus saltatrix)Mar. Freshwater Res.47347355Google Scholar
  10. Howell, W.M., Black, D.A. 1980Controlled silver staining of nucleolus organizer region with protective colloidal developer: a 1-step methodExperientia3610141015PubMedGoogle Scholar
  11. Hunziker, R. 1992The littlest angelsTropical Fish Hobbyist418092Google Scholar
  12. Johnson, G.D., Gill, A.C. 1995Perches and their alliesPaxton, J.R.Eschemeyer, W.N. eds. Encyclopedia of FishesAcademic Press Inc.San Diego181196Google Scholar
  13. Leah, R., Frederiksen, S., Engberg, J., Sorensen, P.D. 1990Nucleotide sequence of a mouse 5S rRNA variant geneNucl. Acids Res.1874417441PubMedGoogle Scholar
  14. Levan, A., Fredga, K., Sandberg, A.A. 1964Nomenclature for centromeric position on chromosomesHereditas52201220CrossRefGoogle Scholar
  15. Margarido, V.P., Galetti, P.M.,Jr 2000Amplification of a GC-rich heterochromatin in the freshwater fish Leporinus desmotes (Characiformes, Anostomidae)Geneti. Mol. Biol.23569573Google Scholar
  16. Martins, C., Galetti, P.M.,Jr 1999Chromosomal localization of 5S rDNA genes in Leporinus fish (Anostomidae, Characiformes)Chrom. Res.7363367PubMedGoogle Scholar
  17. Martins, C., Galetti, P.M.,Jr 2001Two 5S rDNA arrays in Neotropical fish species: is it a general rule for fishes?Genetica111439446PubMedGoogle Scholar
  18. Martins, C., Wasko, A.P., Oliveira, C., Porto-Foresti, F., Parise-Maltempi, P.P., Wright, J.M., Foresti, F. 2002Dynamics of 5S rDNA in the tilapia (Oreochromis niloticus) genome: repeat units, inverted sequences, pseudogenes and chromosome lociCytogenet. Genome Res.987885PubMedGoogle Scholar
  19. Molina, W.F., Galetti, P.M.,Jr 2002Robertsonian rearrangements in the reef fish Chromis (Perciformes, Pomacentridae) involving chromosomes bearing 5S rRNA genesGenet. Mol. Biol.24373377Google Scholar
  20. Molina, W.F., Maia-Lima, F.A., Affonso, P.R.A.M. 2002Divergence between karyotypical pattern and speciation events in Serranidae fish (Perciformes)Caryologia55299305Google Scholar
  21. Pinkel, D., Straume, T., Gray, J.W. 1986Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridizationProc. Natl. Acad. Sci.8329342938PubMedGoogle Scholar
  22. Pyle, R.L. 1990 Centropyge debelius, a new species of angelfish (Teleostei: Pomacanthidae) from Mauritius and ReunionRev. Fr. Aquariol175356Google Scholar
  23. Pyle, R.L., Randall, J.E. 1993A new species of Centropyge from Cook Islands, with a redescription of Centropyge boylei Rev. Fr. Aquariol19115124Google Scholar
  24. Schweizer, D. 1976Reverse fluorescent chromosome banding with chromomycin and DAPIChromosoma92143148Google Scholar
  25. Schweizer, D., Loidl, J., Hamilton, B. 1987Heterochromatin and the phenomenon of chromosome bandingHennig, W. eds. Results and Problems in Cell Differentiation 14: Structure and Function of Eukaryotic Chromosomes.Springer-VerlagBerlinGoogle Scholar
  26. Sola, L., Rossi, A.R., Annesi, F., Gornung, E. 2003Cytogenetic studies in Sparus auratus (Pisces, Perciformes): molecular organization of 5S rDNA and chromosomal mapping of 5S and 45S ribosomal genes and of telomeric repeatsHereditas139232236PubMedGoogle Scholar
  27. Souza, I.L, Moreira-Filho, O., Galetti, P.M.,Jr 1996Heterochromatin differentiation in the characid fish Astyanax scapripinnis Brazilian J. Genet.19405410Google Scholar
  28. Sumner, A. 1972A single technique for demonstrating centromeric heterochromatinExp. Cell Res.75304306PubMedGoogle Scholar
  29. Vicente, V.E., Jesus, C.M., Moreira-Filho, O. 2001Chromosomal localization of 5S and 18S rRNA genes in three Parodon species (Pisces, Parodontidae)Caryologia54365359Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Paulo Roberto Antunes de Mello Affonso
    • 1
  • Pedro Manoel GalettiJr
    • 1
  1. 1.Departamento de Genética e EvoluçãoUniversidade Federal de São CarlosSão Carlos SPBrazil

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