, Volume 123, Issue 3, pp 245–253 | Cite as

Molecular and chromosomal analysis of ribosomal cistrons in two cartilaginous fish, Taeniura lymma and Raja montagui (Chondrichthyes, Batoidea)

  • L. Rocco
  • D. Costagliola
  • M. Fiorillo
  • F. Tinti
  • V. Stingo


We used silver nitrate staining, CMA3 and FISH to study the chromosomal localization of both the major ribosomal genes and the nucleolar organizer regions as well as that of the minor ribosomal genes (5S rDNA) in two species of Batoidea, Taeniura lymma (Dasyatidae) and Raja montagui (Rajidae). In both species, all the metaphases examined showed the presence of multiple NOR-bearing sites, while the gene for 5S rRNA proved to be localized on two chromosome pairs. Furthermore, one of the two 5S rDNA sites in T. lymma was shown to be co-localized with the major ribosomal cluster. The presence of multiple nucleolar organizer regions in the two species might be interpreted as being the result of intraspecific polymorphisms, or as a phenomenon of the amplified transposition of mobile elements of the genome. We also determined the nucleotide sequence of the 5S rRNA gene, consisting of 564 bp in R. montagui and 612 bp in T. lymma. We also found TATA-like and (TGC) n trinucleotides, (CA) n dinucleotides and (GTGA) n tetranucleotides, which probably influence gene regulation.


5S rDNA cartilaginous fish chromosome markers FISH nucleolar organizer region ribosomal genes 



base pair(s)


chromomycin A3


fluorescent in situ hybridization


nucleolar organizer region


non transcribed sequences


phosphate buffer saline


polymerase chain reaction


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  1. Almeida-Toledo, L.F., Stocker, A.J., Foresti, F., Toledo Filho, S.A. 1996Fluorescence in situ hybridization with rDNA probes on chromosomes of two nucleolus organizer region phenotypes of a species of Eigenmannia(Pisces, Gymnotoidei, Sternopygidae)Chromosome Res.4301305PubMedGoogle Scholar
  2. Amarisinghe, V., Carlson, J.E. 1998Physical mapping and characterization of 5S rRNA genes in Douglas-firAm. Gen. Assoc.89495500Google Scholar
  3. Eickbush, D.G., Eickbush, T.H. 1995Vertical transmission of the retrotransposable elements R1 and R2 during the evolution of the Drosophila melanogaster species subgroupGenetics139671684PubMedGoogle Scholar
  4. Fedoroff, N.V. 1979aDeletion mutants of Xenopus laevis 5S ribosomal DNACell16551563Google Scholar
  5. Fedoroff, N.V. 1979bOn spacersCell16697710Google Scholar
  6. Frederiksen, S., Cao, B., Lomholt, G., Levan Hallemberg, C. 1997The rat 5S rRNA bonafide gene repeat maps to chromosome 19q12-qter and the pseudogene repeat maps to12q12Cytogenet. Cell Genet.76101106PubMedGoogle Scholar
  7. Fujiwara, A., Abe, S., Yamaha, E., Yamazaki, F., Yoshida, M.C. 1998Chromosomal localization and heterochromatin association of ribosomal RNA gene loci and silver-stained nucleolar organizer regions in salmonid fishesChromosome Res.6463471PubMedGoogle Scholar
  8. Inafuku, J., Nabeyama, M., Kikuma, Y., Saitoh, J., Kukubota, S., Kohono, S. 2000Chromosomal location and nucleotide sequences of 5S ribosomal DNA of two cyprinid species (Osteichyes, Pisces)Chromosome Res.8193199PubMedGoogle Scholar
  9. Little, R.D., Braaten, D.C. 1989Genomic organization of human 5S rDNA and sequences of one tandem repeatGenomics4376383PubMedGoogle Scholar
  10. Long, E.O., David, I.D. 1980Repeated genes in eucaryotesAnnu. Rev. Biochem.49727764PubMedGoogle Scholar
  11. Lucchini, S., Nardi, I., Barsacchi, G., Batistoni, R., Andronico, F. 1993Molecular cytogenetics of the ribosomal (18S + 28S and 5S) DNA loci in primitive and advanced urodele amphibiansGenome36762773PubMedGoogle Scholar
  12. Mäkinem, A., Zijlstra, C., De Haan, N.A., Mellink, C.H.M., Bosma, A.A. 1997Localization of 18S plus 28S and 5S ribosomal genes in the dog by fluorescence in situ hybridizationCytogenet. Cell Genet.78231235Google Scholar
  13. Martins, C., Galetti, P.M. 1999Chromosomal localization of 5S rDNA genes in Leporinus fish (Anastomidae, Chraciformes)Chromosome Res.7363367PubMedGoogle Scholar
  14. Martins, C., Galetti, P.M. 2001aOrganization of 5S rDNA in Leporinus fish species: two different genomic locations are characterized by distinct non-transcribed spacers (NTSs)Genome44903910Google Scholar
  15. Martins, C., Galetti, P.M. 2001bTwo 5S rDNA array in Neotropical fish species: is it a general rule for fishes?Genetica111439446Google Scholar
  16. Mellink, C.H.M., Bosma, A.A., Haan, N.A., Zijlstra, C. 1996Physical of 5S rRNA genes in the pig by fluorescence in situ hybridationHereditas1249597PubMedGoogle Scholar
  17. Nederby-Nielsen, J., Hallenberg, C., Frederiksen, S., Sorensen, P.D., Lomholt, B.,  et al. 1993Transcription of human 5S rRNA genes is influenced by upstream DNA sequenceNucleic Acids Res.2636313636Google Scholar
  18. Oliveira, C., Foresti, F., Rigolino, M.G., Tabata, Y.A. 1995Paracentric inversion involving a NOR-bearing chromosome of rainbow trout (Oncorhyncus mykiss): electron microscopy studies of the synaptonemal complexChromosome Res.35758Google Scholar
  19. Olmo, E., Stingo, V., Cobror, O., Capriglione, T., Odierna, G. 1982Repetitive DNA and polyploidy in selachiansComp. Biochem. Physiol. B73739745PubMedGoogle Scholar
  20. Pendas, A.M., Moran, P., Freije, J.P., Garcia-Vazquez, E. 1994Chromosomal mapping and nucleotide sequence of two tandem repeats of Atlantic salmon 5S rDNACytogenet. Cell Genet.673136PubMedGoogle Scholar
  21. Phillips, R.B., Zajicek, K.D., Ihssen, P.E. 1989Population differences in chromosome-banding polymorphisms in lake troutTrans. Am. Fish Soc.1186473Google Scholar
  22. Rocco L., Costagliola D., Marsilio A., Stingo V., (2002b) Localizzazione cromosomica dei cistroni ribosomali in due Batoidea. Atti 63° Convegno UZI: 120Google Scholar
  23. Rocco, L., Morescalchi, M.A., Costagliola, D., Stingo, V. 2002Karyotype and genome characterization in four cartilaginous fishesGene295289298PubMedGoogle Scholar
  24. Rocco, L., Russo, C., Stingo, V., Aprea, G., Odierna, G. 1999Charecterization of 5S rDNA in Gasterosteus aculeatus (Teleostei, Gasterosteidae)Ital. J. Zool.66285289CrossRefGoogle Scholar
  25. Rocco, L., Stingo, V., Bellitti, M. 1996Cloning and characterization of a repetitive DNA detected by HindIII in the genome of Raja montagui (Batoidea, Chondrichthyes)Gene176185189PubMedGoogle Scholar
  26. Sajdak, S.L., Reed, K.M., Phillips, R.B. 1998Intraindividual and interspecies variation in the 5S rDNA of Coregonid fishJ. Mol. Evol.46680688PubMedGoogle Scholar
  27. Schmid, M., Vitelli, L., Batistoni, R. 1987Chromosome banding in Amphiba. IV. Constitutive heterochromatin, nucleolus organizers, 18S + 28S and 5S ribosomal RNA genes in Ascaphidae, Pipidae, Discoglossidae and PelobatidaeChromosoma95271284PubMedGoogle Scholar
  28. Srivastava, A.K., Schlessinger, D. 1991Structure and organization of ribosomal DNABiochimie73631638PubMedGoogle Scholar
  29. Stingo, V., Rocco, L. 1991Chondrichthyan cytogenetics: a comparison with TeleosteansJ. Mol. Evol.337682Google Scholar
  30. Stingo, V., Rocco, L. 2001Selachian cytogenetics: a reviewGenetica111329347PubMedGoogle Scholar
  31. Stingo, V., Rocco, L., Improta, R. 1989Chromosome markers and karyology of SelachiansJ. Exp. Zool.2175185Google Scholar
  32. Stingo, V., Rocco, L., Odierna, G., Bellitti, M. 1995NOR and heterochromatin analysis in two cartilaginous fishes by C-, Ag- and RE (restriction endonuclease)-bandingCytogenet. Cell Genet.71228234PubMedGoogle Scholar
  33. Suzuki, H., Sakurai, S., Matsuda, Y. 1996Rat rDNA spacer sequences and chromosomal assignment of the genes to the extreme terminal region of chromosome 19Cytogenet. Cell Genet.7214CrossRefPubMedGoogle Scholar
  34. Vitelli, L., Batistoni, R., Andronico, F., Nardi, I., Barsacchi– Pilone, G. 1982Chromosomal localization of 18S + 28S and 5S ribosomal RNA genes in evolutionary divergent anuran amphibiansChromosoma84475491PubMedGoogle Scholar
  35. Williams, S.M., Strobeck, C. 1985Sister chromatid exchange and the evolution of rDNA spacer lengthJ. Theor. Biol.116625636PubMedGoogle Scholar
  36. Zhuo, L., Reed, K.M., Phillips, R.B. 1995Hypervariability of ribosomal DNA at multiple chromosomal sites in lake trout (Salvelinus namaycush).Genome38487496PubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • L. Rocco
    • 1
  • D. Costagliola
    • 1
  • M. Fiorillo
    • 1
  • F. Tinti
    • 2
  • V. Stingo
    • 1
  1. 1.Department of Life SciencesSecond University of NaplesCasertaItaly
  2. 2.Molecular Genetics for Environmental & Fishery Resources LaboratoryInterdept. Center for Research in Environmental SciencesRavennaItaly

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