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Genetica

, Volume 123, Issue 3, pp 245–253 | Cite as

Molecular and chromosomal analysis of ribosomal cistrons in two cartilaginous fish, Taeniura lymma and Raja montagui (Chondrichthyes, Batoidea)

  • L. Rocco
  • D. Costagliola
  • M. Fiorillo
  • F. Tinti
  • V. Stingo
Article

Abstract

We used silver nitrate staining, CMA3 and FISH to study the chromosomal localization of both the major ribosomal genes and the nucleolar organizer regions as well as that of the minor ribosomal genes (5S rDNA) in two species of Batoidea, Taeniura lymma (Dasyatidae) and Raja montagui (Rajidae). In both species, all the metaphases examined showed the presence of multiple NOR-bearing sites, while the gene for 5S rRNA proved to be localized on two chromosome pairs. Furthermore, one of the two 5S rDNA sites in T. lymma was shown to be co-localized with the major ribosomal cluster. The presence of multiple nucleolar organizer regions in the two species might be interpreted as being the result of intraspecific polymorphisms, or as a phenomenon of the amplified transposition of mobile elements of the genome. We also determined the nucleotide sequence of the 5S rRNA gene, consisting of 564 bp in R. montagui and 612 bp in T. lymma. We also found TATA-like and (TGC) n trinucleotides, (CA) n dinucleotides and (GTGA) n tetranucleotides, which probably influence gene regulation.

Keywords

5S rDNA cartilaginous fish chromosome markers FISH nucleolar organizer region ribosomal genes 

Abbreviations

bp

base pair(s)

CMA3

chromomycin A3

FISH

fluorescent in situ hybridization

NOR

nucleolar organizer region

NTS

non transcribed sequences

PBS

phosphate buffer saline

PCR

polymerase chain reaction

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Copyright information

© Springer 2005

Authors and Affiliations

  • L. Rocco
    • 1
  • D. Costagliola
    • 1
  • M. Fiorillo
    • 1
  • F. Tinti
    • 2
  • V. Stingo
    • 1
  1. 1.Department of Life SciencesSecond University of NaplesCasertaItaly
  2. 2.Molecular Genetics for Environmental & Fishery Resources LaboratoryInterdept. Center for Research in Environmental SciencesRavennaItaly

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