, Volume 123, Issue 3, pp 211–216 | Cite as

Conserved 5S and variable 45S rDNA chromosomal localisation revealed by FISH in Astyanax scabripinnis (Pisces, Characidae)

  • Monique Mantovani
  • Luciano Douglas dos
  • Santos Abel
  • Orlando Moreira-Filho


Fluorescence in situhybridisation (FISH) and double FISH experiments were carried out to ascertain the chromosomal distribution pattern of the 45S and 5S ribosomal (r) DNAs in four populations of the characid fish Astyanax scabripinnis – a group considered to be a species complex for its wide karyotypical and morphological diversity. The results regarding the 45S rDNA agreed with this hypothesis, since these sites showed intra- and inter-populational, numerical and positional variations. However, the data obtained with the 5S rDNA probe revealed a highly conserved chromosomal distribution pattern of these sequences among individuals of each population, as well as among the populations analysed. We consider this contrasting situation as a functional divergence between 45S and 5S ribosomal DNAs, which may reflect the localisation of these sequences in distinct nuclear compartments, leading them to undergo differentiated evolutionary processes.


double FISH fluorescence in situ hybridisation (FISH) karyotype evolution nucleus functional compartmentalisation ribosomal DNAs 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Almeida-Toledo, L.F., Ozouf-Costaz, C., Foresti, F., Bonillo, C., Porto-Foresti, F., Daniel-Silva, M.Z.F. 2002Conservation of the 5S-bearing chromosome pair and co-localization with major rDNA clusters in five species of Astyanax (Pisces, Characidae)Cytogenet. Genome Res.97229233PubMedGoogle Scholar
  2. Bertollo, L.A.C., Takahashi, C.S., Moreira-Filho, O. 1978Cytotaxonomic considerations on Hoplias lacerdae (Pisces, Erythrinidae)Brazil. J Genet.1103120Google Scholar
  3. Born, G.G., Bertollo, L.A.C. 2000An XX/XY sex chromosome system in a fish species, Hoplias malabaricus, with a polymorphic NOR bearing X chromosomeChrom Res.8111118PubMedGoogle Scholar
  4. Cremer, T., Cremer, C. 2001Chromosome territories, nuclear architecture and gene regulation in mammalian cellsNat. Rev. Genet.2292301PubMedGoogle Scholar
  5. Cremer, T., Kreth, G., Koester, H., Fink, R.H.A., Heintzmann, R., Cremer, M., Solovei, I., Zink, D., Cremer, C. 2000Chromosome territories, interchromatin domain compartment and nuclear matrix: an integrated view of the functional nuclear architectureCrit. Rev. Eukaryot. Gene Expr.10179212PubMedGoogle Scholar
  6. Deiana, , A.M., , Cau, A., Salvadori, S., Coluccia, E., Cannas, R., Milia, A., Tagliavini, J. 2000Major and 5S ribosomal sequences of the largemouth bass Micropterus salmoides (Perciformes, Centrarchidae) are localized in GC rich regions of the genomeChrom. Res.8213218PubMedGoogle Scholar
  7. Ferro, D.A.M., Néo, D.M., Moreira-Filho, O., Bertollo, L.A.C. 2001Nucleolar organizing regions, 18S and 5S rDNA in Astyanax scabripinnis (Pisces, Characidae): populations distribution and functional diversityGenetica1105562Google Scholar
  8. Fujiwara, A., Abe, S., Yamaha, E., Yamazaki, F., Yoshida, M. 1998Chromosomal localization and heterochromatin association of ribosomal RNA genes loci and silver-stained nucleolar organizer regions in salmonid fishesChrom. Res.6463471PubMedGoogle Scholar
  9. Galetti, P.M.,Jr 1998Chromosome diversity in Neotropical fishes: NOR studiesItal. J. Zool.655356CrossRefGoogle Scholar
  10. Hizume, M. 1994Allodiploid nature of Allium wakegi Araki revealed by genomic in situ hybridization and localization of 5S and 18S rDNAsJpn. J. Genet.69407415PubMedGoogle Scholar
  11. Lamond, A.I., Earnshaw, W.C. 1998Structure and function in the nucleusScience280547553PubMedGoogle Scholar
  12. Maistro, E.L., Foresti, F., Oliveira, C., Almeida-Toledo, L.F. 1992Occurrence of macro B chromosomes in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)Genetica87101106Google Scholar
  13. Maistro, E.L., Oliveira, C., Foresti, F. 1998Comparative cytogenetic and morphological analysis of Astyanax scabripinnis paranae (Pisces, Characidae, Tetragonopterinae)Genet. Mol. Biol.21201206Google Scholar
  14. Maistro, E.L., Oliveira, C., Foresti, F. 2000Sympatric occurrence of two cytotypes of Astyanax scabripinnis (Characiformes, Characidae)Genet. Mol. Biol.23365369Google Scholar
  15. Mantovani, M., Abel, L.D.S., Mestriner, C.A., Moreira-Filho, O. 2000Accentuated polymorphism of heterochromatin and nucleolar organizer regions in Astyanax scabripinnis (Pisces, Characidae): tools for understanding karyotypic evolutionGenetica109161168PubMedGoogle Scholar
  16. Martínez, J.L., Morán, P., García-Vázquez, E., Pendás, A.M. 1996Chromosomal localization of the major and 5S rRNA genes in the European eel (Anguilla anguilla)Cytogenet. Cell Genet.73149152PubMedGoogle Scholar
  17. Martins, C., Galetti, P.M.,Jr 1999Chromosomal localization of 5S rDNA genes in Leporinus fish (Anostomidae, Characiformes)Chrom. Res.7363367PubMedGoogle Scholar
  18. Martins, C., Galetti, P.M.,Jr 2000Conservative distribution of 5S rDNA loci in Schizodon(Pisces, Anostomidae) chromosomesChrom. Res.8353355Google Scholar
  19. Martins, C., Galetti, P.M.,Jr 2001Two 5S rDNA arrays in Neotropical fish species: Is it a rule for fishes?Genetica111439446PubMedGoogle Scholar
  20. Miller, D.A., Dev, V.G., Tantravahi, R., Millar, O.J. 1976Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cellsExpl. Cell Res.101235243Google Scholar
  21. Mizoguchi, S.M.H.N., Martins-Santos, I.C. 1998Activation patterns of the nucleolar organizer region in Astyanax scabripinnis populations (Pisces, Characidae)Cytologia63259265Google Scholar
  22. Mizoguchi, S.M.H.N., Martins-Santos, I.C. 1998Cytogenetics and morfometric differences in populations of Astyanax scabripinnis (Pisces, Characidae) from Maringá region, PR, BrazilGenet. Mol. Biol.215561Google Scholar
  23. Moreira-Filho, O., Bertollo, L.A.C. 1991 Astyanax scabripinnis (Pisces, Characidae): a species complexBrazil. J. Genet.14331357Google Scholar
  24. Pendás, A.M., Morán, P., Freije, J.P., García-Vázquez, E. 1994Chromosomal location and nucleotide sequence of two tandem repeats of the Atlantic salmon 5S rDNACytogenet. Cell Genet.673136CrossRefPubMedGoogle Scholar
  25. Pinkel, D., Straume, T., Gray, J.W. 1986Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridizationProc. Natl. Acad. Sci. USA8329342938PubMedGoogle Scholar
  26. Sadoni, N., Langer, S., Fauth, C., Bernardi, G., Cremer, T., Turner, B.M., Zink, D. 1999Nuclear organization of mammalian genomes: polar chromosome territories built up functionally distinct higher order compartmentsJ. Cell Biol.14612111226PubMedGoogle Scholar
  27. Schweizer, D., Loidl, J. 1987A model for heterochromatin dispersion and the evolution of C band patternsChrom. Today96174Google Scholar
  28. Sola, L., De Innocentiis, S., Gornung, E., Papalia, S., Rossi, A.R., Marino, G., De Marco, P., Cataudella, S. 2000Cytogenetic analysis of Epinephenus marginatus (Pisces, Serranidae), with the chromosome localization of the 18S and 5S rRNA genes and of the (TTAGGG)n telomeric sequenceMar. Biol.1374751Google Scholar
  29. Souza, I.L., Moreira-Filho, O. 1995Cytogenetic diversity in the Astyanax scabripinnis species complex (Pisces, Characidae). I. Allopatric distribution in a small streamCytologia60111Google Scholar
  30. Souza, I.L., Moreira-Filho, O., Bertollo, L.A.C. 1995Cytogenetic diversity in the Astyanax scabripinnis species complex (Pisces, Characidae). II. Different cytotypes living in sympatryCytologia60273281Google Scholar
  31. Stange, E.A.R., Almeida-Toledo, L.F. 1993Supernumerary B chromosome restricted to males in Astyanax scabripinnis (Pisces, Characidae)Brazil. J. Genet.16601615Google Scholar
  32. Vicente, V.E., Jesús, C.M., Moreira-Filho, O. 2001Chromosomal localization of 5S and 18S rRNA genes in three Parodon species (Pisces, Parodontidae)Caryologia54365369Google Scholar
  33. Vicente, V.E., Moreira-Filho, O., Camacho, P.M. 1996Sex-ratio distortion associated with the presence of a B chromosome in Astyanax scabripinnis (Teleostei, Characidae)Cytogenet. Cell Genet.747075PubMedGoogle Scholar
  34. Visser, A.E., Aten, J.A. 1999Chromosome as well as chromosomal subdomains constitute distinct units in interphase nucleiJ. Cell Sci.11233533360PubMedGoogle Scholar
  35. White, T.J., Burns, T., Lee, S., Taylor, T. 1990Amplification and direct sequencing of fungal ribosomal RNA genes for phylogeneticsInnis, M.A.Gelfand, D.H.Sninsky, J.J.White, T.J. eds. PCR Protocols: A Guide to Methods and ApplicationsAcademic Press IncSan Diego315322Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Monique Mantovani
    • 1
  • Luciano Douglas dos
    • 1
  • Santos Abel
    • 1
  • Orlando Moreira-Filho
    • 1
  1. 1.Departamento de Genética e EvoluçãoUniversidade Federal de São CarlosSão Carlos-SPBrazil

Personalised recommendations