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Genetica

, Volume 123, Issue 3, pp 211–216 | Cite as

Conserved 5S and variable 45S rDNA chromosomal localisation revealed by FISH in Astyanax scabripinnis (Pisces, Characidae)

  • Monique Mantovani
  • Luciano Douglas dos
  • Santos Abel
  • Orlando Moreira-Filho
Article

Abstract

Fluorescence in situhybridisation (FISH) and double FISH experiments were carried out to ascertain the chromosomal distribution pattern of the 45S and 5S ribosomal (r) DNAs in four populations of the characid fish Astyanax scabripinnis – a group considered to be a species complex for its wide karyotypical and morphological diversity. The results regarding the 45S rDNA agreed with this hypothesis, since these sites showed intra- and inter-populational, numerical and positional variations. However, the data obtained with the 5S rDNA probe revealed a highly conserved chromosomal distribution pattern of these sequences among individuals of each population, as well as among the populations analysed. We consider this contrasting situation as a functional divergence between 45S and 5S ribosomal DNAs, which may reflect the localisation of these sequences in distinct nuclear compartments, leading them to undergo differentiated evolutionary processes.

Keywords

double FISH fluorescence in situ hybridisation (FISH) karyotype evolution nucleus functional compartmentalisation ribosomal DNAs 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Monique Mantovani
    • 1
  • Luciano Douglas dos
    • 1
  • Santos Abel
    • 1
  • Orlando Moreira-Filho
    • 1
  1. 1.Departamento de Genética e EvoluçãoUniversidade Federal de São CarlosSão Carlos-SPBrazil

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