Skip to main content
SpringerLink
Log in

Genetic Mapping and ENU Mutagenesis

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

ENU mutagenesis is a potent means to generate novel mutations in the mouse, and the further investigation of these mutations can be logistically demanding. Determination of the map position of a mutation early in its characterization can be extremely useful. We describe how the use of interval haplotype analysis can facilitate this with even small numbers of affected progeny.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bode, V. C., J. D. McDonald, J.-L. Guenet & D. Simon, 1988. hph-1: a mouse mutant with hereditary hyperphenylalaninemia induced by ethylnitrosourea mutagenesis. Genetics 118: 299–305.

    PubMed  Google Scholar 

  • Herron, B. J., W. Lu, C. Rao, S. Liu, H. Peters, R. T. Bronson, M. J. Justice, J. D. McDonald & D. R. Beier, 2002. Efficient generation and mapping of recessive developmental muta-tions using ENU mutagenesis. Nat. Genet. 30: 185–189.

    PubMed  Google Scholar 

  • Lee, E. C., D. Yu, J. Martinez de Velasco, L. Tessarollo, D. A. Swing, D. L. Court, N. A. Jenkins & N. G. Copeland, 2001.A highly effcient Escherichia coli-based chromosome engi-neering system adapted for recombinogenic targeting and subcloning of BAC DNA. Genomics 73: 56–65.

    PubMed  Google Scholar 

  • Mitchell, K. J., K. I. Pinson, O. G. Kelly, J. Brennan, J. Zupicich, P. Scherz, P. A. Leighton, L. V. Goodrich, X. Lu, B. J. Avery, P. Tate, K. Dill, E. Pangilinan, P. Wakenight, M. Tessier-Lavigne & W. C. Skarnes, 2001. Functional analysis of secreted and transmembrane proteins critical to mouse development. Nat. Genet. 28(3): 198–200.

    PubMed  Google Scholar 

  • Nadeau, J. H., J. B. Singer, A. Matin & E. S. Lander, 2000. Analysing complex genetic traits with chromosome substitution strains. Nat. Genet. 24: 221–225.

    PubMed  Google Scholar 

  • Neuhaus, I. & D. Beier, 1998. Efficient localization of muta-tions by interval haplotype analysis. Mammal. Genome 9: 150–154.

    Google Scholar 

  • Russell, W. L., E. M. Kelly, P. R. Hunsicker, J. W. Bangham, S. C. Maddux & E. L. Phipps, 1979. Specific locus test shows ethylnitrosurea to be the most potent mutagen in the mouse. Proc. Natl. Acad. Sci. (USA) 76: 5818–5819.

    Google Scholar 

  • Russell, W. L., P. R. Hunsicker, D. A. Carpenter, C. V. Cornett & G. M. Guinn, 1982. Effect of dose-fractionation on the ethylnitrosurea induction of specific-locus mutations in mouse spermatogonia. Proc. Natl. Acad. Sci. (USA) 79: 3592–3593.

    Google Scholar 

  • Sokal, R. S. & F. J. Rohlf, 1981. Biometry, Freeman, New York.

    Google Scholar 

  • Takeshima, H., M. Iino, H. Takekura, M. Nishi, J. Kuno, O. Minowa, H. Takano & T. Noda, 1994. Excitation-contraction uncoupling and muscular degeneration in mice lacking functional skeletal muscle ryanodine-receptor gene. Nature 369: 556–559.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Genetics Division, Brigham and Women’s Hospital, Harvard Medical School, MA, USA

    David R. Beier & Bruce J. Herron

Authors
  1. David R. Beier
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bruce J. Herron
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Beier, D.R., Herron, B.J. Genetic Mapping and ENU Mutagenesis. Genetica 122, 65–69 (2004). https://doi.org/10.1007/s10709-004-1437-5

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10709-004-1437-5

Search

Navigation