Abstract
Insulin-like growth factor-binding proteins (IGFBPs) play important roles in regulating growth and development by binding to IGF, where IGFBP-3 and IGFBP-5 are the main binding carriers of IGF in the circulation system. In the present study, the gene sequences of igfbp-3, igfbp-5a, and igfbp-5b were cloned from the liver of yellowtail kingfish (Seriola lalandi). The ORF sequences of igfbp-3, igfbp-5a, and igfbp-5b were 888, 801, and 804 bp in length, which encoded 295, 266, and 267 amino acids, respectively. The above three genes were widely expressed in yellowtail kingfish tissues, with igfbp-3 being the most highly expressed in the heart, brain, and gonads, while igfbp-5a and igfbp-5b were both most highly expressed in the liver and kidney. The expression levels of igfbp-3, igfbp-5a, and igfbp-5b were detected throughout the embryonic and larval stages, suggesting their roles in early development and growth regulation of yellowtail kingfish. Besides, igfbp-3 and igfbp-5a were significantly up-regulated in the liver under food deprivation and high-density rearing conditions, which was exactly opposite to the growth performance of yellowtail kingfish, implying that they may serve as biomarkers of adverse culture conditions. Overall, the above results initially identified the molecular characteristics of igfbp-3/-5a/-5b in yellowtail kingfish and implied that they might play important roles in the growth and development, providing a basis for further research on underlying regulatory mechanisms.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Funding
This research was supported by the Marine Science & Technology Fund of Shandong Province for Qingdao Marine Science and Technology Center (2022QNLM030001), Key R&D Program of Shandong Province (2023TZXD050), Taishan Industrial Experts Program, Central Public-interest Scientific Institution Basal Research Fund, CAFS (2023TD51; 2024XT0701) and Earmarked Fund for China Agriculture Research System (CARS-47).
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Jichang Zheng and Wenjing Zhang wrote the main manuscript. Yongjiang Xu provided the fund and revised the manuscript. Aijun Cui and Yan Jiang were responsible for designing the experiment. Bin Wang performed the formal analysis. All the authors read and approved the final manuscript.
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Zheng, J., Zhang, W., Xu, Y. et al. Insulin-like growth factor binding protein-3 (igfbp-3) and igfbp-5 in yellowtail kingfish (Seriola lalandi): molecular characterization and expression levels under different nutritional status and stocking density. Fish Physiol Biochem (2024). https://doi.org/10.1007/s10695-024-01359-w
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DOI: https://doi.org/10.1007/s10695-024-01359-w