Abstract
Peroxisome proliferator–activated receptor β (pparβ) is a key gene–regulating lipid metabolism pathway, but its function in turbot remains unclear. In this study, the CDS of pparβ was cloned from kidney for the first time. The CDS sequence length was 1533 bp encoding 510 amino acids. Structural analysis showed that the pparβ protein contained a C4 zinc finger and HOLI domain, suggesting that the pparβ gene of turbot has high homology with the PPAR gene of other species. The high expression patterns of pparβ, acox, and cpt-1 at high temperatures, as shown through qPCR, indicated that high temperatures activated the transcriptional activity of pparβ and increased the activity of the acox and cpt-1 genes. The expression of acox and cpt-1 was significantly inhibited when pparβ was downregulated using RNAi technology and inhibitor treatments, suggesting that pparβ positively regulated acox and cpt-1 expression at high temperatures and, thus, modulates lipid catabolism activity. These results demonstrate that pparβ is involved in the regulation of lipid metabolism at high temperatures and expand a new perspective for studying the regulation of lipid metabolism in stress environments of teleost.
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Data availability
All data generated and analyzed during this study are included in this published article and its supplementary information files. The gene sequence has been submitted to NCBI, GenBank accession number MT275452.
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Tingting Zhao: conceptualization, methodology, writing—original draft. Aijun Ma: conceptualization, methodology. Zhihui Huang: methodology. Zhifeng Liu: writing—review and editing. Zhibin Sun: software. Liguang Zhu and Haowen Chang: resources.
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All the experimental animal programs involved in this study were approved by the Yellow Sea Fisheries Research Institute’s animal care and use committee (ID Number: YSFRI-2021026), and followed basic experimental principles.
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Highlights
•A CDS sequence of pparβ was identified and characterized from turbot, Scophthalmus maximus.
•High temperature induced the expression of pparβ and activated key genes in lipid catabolism, acox and cpt-1.
•We used RNAi techniques to demonstrate that pparβ regulates lipid catabolism.
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Zhao, T., Ma, A., Huang, Z. et al. pparβ regulates lipid catabolism by mediating acox and cpt-1 genes in Scophthalmus maximus under heat stress. Fish Physiol Biochem 50, 295–305 (2024). https://doi.org/10.1007/s10695-024-01313-w
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DOI: https://doi.org/10.1007/s10695-024-01313-w