Abstract
Investigated mitigating effects of sodium butyrate (SB) on the inflammatory response, oxidative stress, and growth inhibition of common carp (Cyprinus carpio) (2.94 ± 0.2 g) are caused by glycinin. Six isonitrogenous and isoenergetic diets were prepared, in which the basal diet was the control diet and the Gly group diet contained 80 g/kg glycinin, while the remaining 4 diets were supplemented with 0.75, 1.50, 2.25, and 3.00 g/kg SB, respectively. The feeding trial lasted for 8 weeks, and the results indicated that supplementing the diet with 1.50–2.25 g/kg of SB significantly improved feed efficiency and alleviated the growth inhibition induced by glycinin. Hepatopancreas and intestinal protease activities and the content of muscle crude protein were significantly decreased by dietary glycinin, but supplement 1.50–2.25 g/kg SB partially reversed this result. SB (1.50–2.25 g/kg) increased the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the hepatopancreas and reduced the activities of AST and ALT in the serum. Glycinin significantly reduced immune and antioxidant enzyme activities, whereas 1.50–2.25 g/kg SB reversed these adverse effects. Furthermore, compared with the Gly group, supplement 1.50–2.25 g/kg SB eminently up-regulated the TGF-β and IL-10 mRNA, and down-regulated the IL-1β, TNF-α, and NF-κB mRNA in hepatopancreas, mid-intestine (MI), and distal intestine (DI). Meanwhile, supplement 1.50–2.25 g/kg SB activated the Keap1-Nrf2-ARE signaling pathway and upregulate CAT, SOD, and HO-1 mRNA expression in hepatopancreas, MI, and DI. Summarily, glycinin induced inflammatory response, and oxidative stress of common carp ultimately decreased the digestive function and growth performance. SB partially mitigated these adverse effects by activating the Keap1-Nrf2-ARE signaling pathway and inhibiting the NF-κB signaling pathway.
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The data supporting the results of this study are available from the corresponding author upon request.
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Jilin Science and Technology Innovation and Entrepreneurship Excellence for Young and Middle-aged People (Team) Project (Innovation Category), No. 20230508008RC.
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Deng-Lai Li carried out the experiments, analyzed the data, and wrote the manuscript. Si-Ying Liu, Rui Zhu, Si-Tong Meng, Yin-Tao Wang, Zhi-Yong Yang, Liang Li, Guo-Jun Shang, Xiao-Fang Wei, Hao-Tong Wang, Zi-Hui Qu, and Ya-Nan Quan sampled and contributed reagents/materials. Li-Fang Wu conceived the study and designed the experiments. All authors read and approved the final manuscript. All authors read and approved the final manuscript.
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This study was using only laboratory animals that were handled according to approval by the Institutional Animal Care and Use Committee of Jilin Agricultural University (approval number: 20220923004).
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Li, DL., Liu, SY., Zhu, R. et al. Potential protective effects of sodium butyrate on glycinin-induced oxidative stress, inflammatory response, and growth inhibition in Cyprinus carpio. Fish Physiol Biochem 50, 273–293 (2024). https://doi.org/10.1007/s10695-023-01276-4
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DOI: https://doi.org/10.1007/s10695-023-01276-4