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lpla (lipoprotein lipase a) is a marker of early adipogenesis rather than late adipogenesis in grass carp (Ctenopharyngodon idellus)

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An Author Correction to this article was published on 14 November 2023

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Abstract

Lipoprotein lipase (LPL) functions as a marker of adipocyte differentiation in mammals, but little is known about its role in fish adipogenesis. The aim of this research is to investigate the function of Lpl in adipocyte differentiation in fish. In this paper, we isolated and characterized lipoprotein lipase a (lpla) and lipoprotein lipase b (lplb) from grass carp (Ctenopharyngodon idellus). The complete coding sequence of lpla and lplb was 1524 bp and 1503 bp in length, coding for 507 amino acids and 500 amino acids, respectively. Both lpla and lplb mRNA were expressed in a great number of tissues. During adipogenesis, the level of lpla mRNA reached its maximum at day 2 and then dropped gradually, while the level of lplb mRNA had no significant changes, indicating that lpla and lplb may have different function in the differentiation of grass carp adipocyte. Furthermore, inhibition of lpla by inhibitor of LPL(GSK264220A) at early time points most clearly reduced adipogenesis, whereas these effects were less pronounced at later stages, suggesting that lpla predominantly affects early adipogenesis rather than late adipogenesis. Based on these findings, it can be inferred that lpla and lplb in grass carp may have distinct roles in the differentiation of grass carp adipocyte, and lpla may play an important role in the early adipogenesis rather than late adipogenesis in grass carp.

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Acknowledgements

The authors would like to thank Ph. D Jishu Zhou for her help in the experiment.

Funding

This work was financially supported by the National Natural Science Foundation of China (NSFC) (32002403), China Postdoctoral Science Foundation Funded Project (2019M660266).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, Xianyang, Yangling, China

    Zhiqi Tian, Mingkui Wei, Rongrong Xue, Lei Song, Handong Li, Hong Ji & Jian Sun

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  1. Zhiqi Tian
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  2. Mingkui Wei
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  3. Rongrong Xue
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  4. Lei Song
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  5. Handong Li
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  7. Jian Sun
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Contributions

Jian Sun and Hong Ji proposed the study and designed the project. Zhiqi Tian wrote the paper. Rongrong Xue and Lei Song analyzed the data by statistical methods, prepared figures and legends. Mingkui Wei and Handong Li revised the literature and contributed to the discussion. All authors revised and edited the manuscript.

Corresponding author

Correspondence to Jian Sun.

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Ethics approval

We promised that all procedures of the experiment were performed in accordance with the Guide for Care and Use of Laboratory Animals and approved by the Northwest A&F University Institutional Animal Care and Use Committee.

Competing interests

We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, and there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the content of this paper. The manuscript has been reviewed, approved, and contributed significantly to the paper by all authors.

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The original online version of this article was revised: Because of a mathematical error in the calculation, the number of the complete coding sequence of lpla and lplb in the abstract of the above paper was incorrect. This error does not affect any of the data, figures, or the interpretation of the data. The correct information given below.

The complete coding sequence of lpla and lplb was 1524 bp and 1503 bp in length, coding for 507 amino acids and 500 amino acids, respectively.

Supplementary information

ESM 1:

Figure S1. Uncropped gels for evaluating the integrity of the RNA that used to identify and clone of lpl. Figure S2. Uncropped gels for evaluating the PCR products of lpl. (DOCX 551 kb)

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Tian, Z., Wei, M., Xue, R. et al. lpla (lipoprotein lipase a) is a marker of early adipogenesis rather than late adipogenesis in grass carp (Ctenopharyngodon idellus). Fish Physiol Biochem 49, 1229–1239 (2023). https://doi.org/10.1007/s10695-023-01253-x

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  • DOI: https://doi.org/10.1007/s10695-023-01253-x

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