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Discovery, characterization, and adipocyte differentiation regulation in perirenal adipose tissue of large yellow croaker (Larimichthys crocea)

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Abstract

Adipose tissue is an essential tissue for lipid deposition in fish and is associated with excess lipid accumulation in aquaculture. However, the knowledge of the distribution and characterization of adipose tissue in fish still needs further investigation. This study for the first time discovered perirenal adipose tissue (PAT) in large yellow croaker by MRI and CT technologies. Then, the morphological and cytological characteristics of PAT were observed, showing a typical characteristic of white adipose tissue. Meanwhile, the mRNA expression of marker genes of white adipose tissue was highly expressed in PAT compared with the liver and muscle in large yellow croaker. Moreover, based on the discovery of PAT, preadipocytes from PAT were isolated, and the differentiation system of preadipocytes was established. The lipid droplet and TG content of cell were gradually increased during adipocyte differentiation. In addition, mRNA expressions of lipoprotein lipase, adipose triglyceride lipase, and transcription factors related to adipogenesis (cebpα, srebp1, pparα, and pparγ) were quantified to explain the regulation mechanism during the differentiation process. In summary, the present study first discovered perirenal adipose tissue in fish, then explored the characterization of PAT, and revealed the regulation of adipocyte differentiation. These results could advance the understanding of adipose tissue in fish and provide a novel idea for the study of the mechanism of lipid accumulation.

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Data availability

The datasets generated during or analyzed during the current study are available from the corresponding author on reasonable request.

Code availability

Not applicable.

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Funding

This work was supported by the Scientific and Technological Innovation of Blue Granary (Grant No. 2018YFD0900402), the Key Program of National Natural Science Foundation of China (Grant No. 31830103), the National Science Fund for Distinguished Young Scholars of China (Grant No. 31525024), the Ten-thousand Talents Program (Grant No. 2018–29), and the earmarked fund for CARS-47.

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Authors and Affiliations

  1. Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong, People’s Republic of China

    Dan Xu, Ye Gong, Xiaojun Xiang, Yongtao Liu, Kangsen Mai & Qinghui Ai

  2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, 266237, Qingdao, Shandong, People’s Republic of China

    Kangsen Mai & Qinghui Ai

Authors
  1. Dan Xu
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  2. Ye Gong
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  4. Yongtao Liu
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  5. Kangsen Mai
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  6. Qinghui Ai
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Contributions

Dan Xu: Conceptualization, Data curation, Formal analysis, Methodology, Visualization, Writing - review &editing. Ye Gong: Investigation, Validation, Writing - original draft. Xiaojun Xiang: Software, Methodology, Writing - review & editing. Yongtao Liu: Visualization, Software. Kangsen Mai: Data curation, Resources, Supervision. Qinghui Ai: Data curation, Resources, Writing - review & editing, Supervision, Project administration, Funding acquisition. All authors reviewed the manuscript.

Corresponding author

Correspondence to Qinghui Ai.

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All experiments in this study were in accordance with the Management Rule of Laboratory Animals (Chinese Order No. 676 of the State Council, revised on 1 March 2017). The authors agree to collaborate and publish this article.

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Xu, D., Gong, Y., Xiang, X. et al. Discovery, characterization, and adipocyte differentiation regulation in perirenal adipose tissue of large yellow croaker (Larimichthys crocea). Fish Physiol Biochem 49, 627–639 (2023). https://doi.org/10.1007/s10695-023-01208-2

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