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Different cold tolerances among three strains of large yellow croaker: related to antioxidant defense and energy metabolism

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Abstract

The aim of this study was to compare low-temperature tolerances in different strains of large yellow croaker. Dai Qu (DQ), Min-Yue Dong (MY), and Quan Zhou (NZ) strains of large yellow croaker were subjected to cold stress (8.6 °C) for 12 h, 24 h, 48 h, and 96 h. Survival rate, histological observation, and antioxidant and energy metabolism indicators were determined. The results showed that compared with the DQ group and MY group, NZ group aggravated hepatic structure, enhanced ROS, lactate, and anaerobic metabolism (PK gene expression and activity), while inhibited ATP, GSH, antioxidant enzymes (mRNA levels and activities of SOD, GPx, and CAT), and aerobic metabolism enzymes (mRNA levels and activities of F-ATPase, SDH, and MDH), indicating the reduction of cold tolerance in the NZ group was closely correlated with the decrement of antioxidative capacity and energy metabolism efficiency. Nrf2 and AMPK gene expressions were correlated with antioxidant and energy metabolism mRNA levels, respectively, suggesting Nrf2 and AMPK might participate in the modulation of target genes during the cold-stress adaptation. In conclusion, low temperature tolerance of fish depended on the antioxidant defense and energy metabolism efficiency, which contributes to understanding the underlying mechanisms of cold adaptation in large yellow croaker.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This study was supported by the Natural Science Foundation of Fujian Province (2022J01027), Natural Science Foundation of Zhejiang Province (LY18D060008), and Key research projects of universities of Anhui Education Department (2022AH051923).

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Authors and Affiliations

  1. College of Food and Bioengineering, Bengbu University, Bengbu, 233030, People’s Republic of China

    Lin Zeng & Yong-Hong Wang

  2. College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361005, China

    Chun-Xiang Ai & Wei-Guang Zou

  3. National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, 316000, China

    Hui Zhang

  4. Fisheries College of Guangdong Ocean University, Zhanjiang, 524088, People’s Republic of China

    Yu-Cong Huang

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  1. Lin Zeng
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Yong-Hong Wang, Hui Zhang, Yu-Cong Huang, and Wei-Guang Zou. The first draft of the manuscript was written by Chun-Xiang Ai, the main manuscript was revised by Lin Zeng, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Lin Zeng.

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We assured that all experiments, animal care, and protocols followed the ethical guidelines of the Bengbu University for the care and use of laboratory animals, and also followed the ethical guidelines of the Zhejiang Ocean University for the care and use of laboratory animals.

With the submission of this manuscript “Different cold tolerances among three strains of large yellow croaker: related to antioxidant defense and energy metabolism,” I would like to confirm that the above-mentioned manuscript has not been published elsewhere, accepted for publication elsewhere or under editorial review for publication elsewhere; and that my Institutes’ (Bengbu University and Zhejiang Ocean University) representatives are fully aware of this submission.

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Zeng, L., Wang, YH., Ai, CX. et al. Different cold tolerances among three strains of large yellow croaker: related to antioxidant defense and energy metabolism. Fish Physiol Biochem 49, 471–486 (2023). https://doi.org/10.1007/s10695-023-01201-9

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