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Phylogeny and expression of ADAM10 and ADAM17 homologs in lamprey

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Abstract

The ADAMs (a disintegrin and metalloproteinase) play regulatory roles in cell adhesion, migration and proteolysis. To explore the origin and evolution of ADAMs, this study identified the homologs of adam10 and adam17 in Lampetra morii and Lampetra japonica. Sequence analysis revealed that they share the same genomic structures with their counterparts in jawed vertebrates. The putative proteins possess conserved motifs, including a furin cut site (RXXR) for precursor processing, an enzyme catalytic motif (HEXGEHXXGXXH) for hydrolysis, and a Ca2+-binding motif (CGNXXXEXGEXCD) for stabilizing protein structure. In addition, a substrate recognition domain is present at the membrane-proximal region of lamprey ADAM17. The cytoplasmic region of lamprey ADAM10 contains a potential threonine phosphorylation site which has been shown to be activated by protein kinase C (PKC) in mammals. Both the adam10 and adam17 genes were constitutively expressed in the brain, kidney, and gills and were differentially regulated in the primary blood leukocytes by lipopolysaccharide (LPS) and pokeweed mitogen (PWM). Adam10 was induced by LPS but not PWM; conversely, adam17 was induced by PWM but not LPS. Taken together, our results suggest that the activation pathways and functions of ADAM10 and ADAM17 are conserved in agnathans.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding authors on reasonable request.

Code availability

Not applicable.

Abbreviations

BCR:

B-cell receptor

CDS:

Complete coding sequence

EGF:

Epidermal growth factor

ErbB:

Epidermal growth factor receptor

ERKs:

Extracellular regulated protein kinases

GSP:

Gene-specific primer

LPS:

Lipopolysaccharide

MHC:

Major histocompatibility complex

MPD:

Membrane-proximal domain

NICD:

Notch intracellular domain

PAMP:

Pathogen-associated molecular pattern

PWM:

Pokeweed mitogen

RACE:

Rapid amplification of cDNA end

TACE:

TNFα-converting enzyme

TCR:

T-cell receptor

TM:

Transmembrane

TNFα:

Tumor necrosis factor alpha

UTR:

Untranslated region

VLR:

Variable lymphocyte receptor

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Funding

This work is funded by the National Key R&D Program of China (Grant No. 2018YFD0900302) and the National Natural Science Foundation of China (Grant Nos. 32030112 and U21A20268).

Author information

Authors and Affiliations

  1. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Ministry of Education), Shanghai Ocean University, Shanghai, 201306, China

    Kaizheng Wu, Jing Xu, Zhao Jia, Junya Wang, Zixuan Wang, Jianhua Feng, Xiaozhen Zhu, Qin Liu, Bangjie Wang, Mingjie Li & Jun Zou

  2. International Research Center for Marine Biosciences at, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China

    Kaizheng Wu, Jing Xu, Zhao Jia, Junya Wang, Zixuan Wang, Jianhua Feng, Xiaozhen Zhu, Qin Liu, Bangjie Wang, Mingjie Li & Jun Zou

  3. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China

    Kaizheng Wu, Jing Xu, Zhao Jia, Junya Wang, Zixuan Wang, Jianhua Feng, Xiaozhen Zhu, Qin Liu, Bangjie Wang, Mingjie Li & Jun Zou

  4. Lamprey Research Center, Liaoning Normal University, Dalian, 116081, China

    Yue Pang

  5. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China

    Jun Zou

Authors
  1. Kaizheng Wu
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  2. Jing Xu
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  3. Zhao Jia
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  7. Xiaozhen Zhu
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  8. Qin Liu
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  9. Bangjie Wang
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  10. Mingjie Li
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  11. Yue Pang
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  12. Jun Zou
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Contributions

Kaizheng Wu, Jing Xu: investigation, methodology, data curation, and writing—original draft. Zhao Jia, Junya Wang, Zixuan Wang, Jianhua Feng, Xiaozhen Zhu, Qin Liu, Bangjie Wang, Mingjie Li: investigation and methodology. Yue Pang: supervision, writing—review and editing. Jun Zou: conceptualization, funding acquisition, project administration, supervision, writing—review and editing.

Corresponding authors

Correspondence to Yue Pang or Jun Zou.

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Experimental procedures were approved by the Animal Welfare and Research Ethics Committee of the Liaoning Normal University and the Institute of Dalian Medical University (Permit number: SYXK2004-0029).

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Wu, K., Xu, J., Jia, Z. et al. Phylogeny and expression of ADAM10 and ADAM17 homologs in lamprey. Fish Physiol Biochem 49, 321–334 (2023). https://doi.org/10.1007/s10695-023-01184-7

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