Abstract
Artemia nauplii are widely used as fish larvae feed due to its beneficial nutritional characteristics for larval development; however, efficient feeding strategies are needed to balance its high costs. Therefore, we evaluated the effects of different densities of Artemia nauplii (100, 250, 500, 750, and 1000 nauplii/post-larvae) on the growth, survival, water quality, and myogenic gene expression of tambaqui (Colossoma macropomum) post-larvae cultivated in a recirculating aquaculture system. After 2 weeks of trial, there was a significant decrease in dissolved oxygen concentration with the increase in nauplii density, but it did not interfere with larval performance and survival. In the first week, larvae fed with fewer than 500 nauplii/post-larvae presented slower growth, while in the second week, larvae fed with 1000 nauplii/post-larvae had the highest final weight and length. Regression analysis suggests that the optimum feeding density of Artemia nauplii during the first week is 411 nauplii/post-larvae, while for the second week, the growth increased proportionally to the feeding densities. The relative expression of the myod, myog, and mstn genes was higher in larvae fed with fewer than 500 nauplii/post-larvae. Although low-growing larvae showed increased expression of myod and myog genes, responsible for muscle hyperplasia and hypertrophy, respectively, mstn expression may have played a significant inhibitory role in larval development. Further research is needed to better determine the effects of the live food on the zootechnical performance and the expression of the myogenic genes in the initial phase of the life cycle of the tambaqui post-larvae.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
We are especially grateful to the professors and technical staff at the LGA, BIOAQUAM, and LSBM for their technical support during the present study.
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This work was supported by the Brazilian Coordination for Higher Education Personnel Training (CAPES) with grant number 88881.200563/2018–01 (PROCAD-AM), grant number 88881.510299/2020–01 (PDPG-Amazônia Legal) and National Council for Scientific and Technological Development (CNPq) with grant number 432209/2018–2 (CNPq/Universal), grant number 314102/2021–3 (CNPq/Productivity in Research).
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Debora Sayumi Doami Melo: Conceptualization, investigation, writing—original draft, visualization. André Luiz Alves de Sá: Writing—review and editing and supervision. Sávio Lucas de Matos Guerreiro: Investigation. Joane Natividade: Investigation. Paola Fabiana Fazzi Gomes: Investigation. Rodrigo Takata: Supervision. Ednaldo da Silva Filho: Resources, writing—review and editing. Glauber David Almeida Palheta: Conceptualization, methodology, and supervision. Nuno Filipe Alves Correia de Melo: Resources. Fabio Carneiro Sterzelecki: Conceptualization, methodology, formal analysis, writing—review and editing, visualization, and project administration. Igor Guerreiro Hamoy: Resources, writing—review and editing, supervision, project administration, funding acquisition.
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Melo, D.S.D., de Sá, A.L.A., de Matos Guerreiro, S.L. et al. Growth, survival, and myogenic gene expression in the post-larvae of Colossoma macropomum provisioned with Artemia nauplii. Fish Physiol Biochem 50, 145–155 (2024). https://doi.org/10.1007/s10695-023-01182-9
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DOI: https://doi.org/10.1007/s10695-023-01182-9