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Intestinal metabolomics of juvenile lenok (Brachymystax lenok) in response to heat stress

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Abstract

Changes in the metabolic profile within the intestine of lenok (Brachymystax lenok) when challenged to acute and lethal heat stress (HS) are studied using no-target HPLC–MS/MS metabonomic analysis. A total of 51 differentially expressed metabolites (VIP > 1, P < 0.05) were identified in response to HS, and 34 occurred in the positive ion mode and 17 in negative ion mode, respectively. After heat stress, changes in metabolites related to glycolysis (i.e., alpha-D-glucose, stachyose, and L-lactate) were identified. The metabolites (acetyl carnitine, palmitoylcarnitine, carnitine, and erucic acid) related to fatty acid β-oxidation accumulated significantly, and many amino acids (L-tryptophan, D-proline, L-leucine, L-phenylalanine, L-aspartate, L-tyrosine, L-methionine, L-histidine, and L-glutamine) were significantly decreased in HS-treated lenok. The mitochondrial β-oxidation pathway might be inhibited, while severe heat stress might activate the anaerobic glycolysis and catabolism of amino acid for energy expenditure. Oxidative damage in HS-treated lenok was indicated by the decreased glycerophospholipid metabolites (i.e., glycerophosphocholine, 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine, 1-palmitoyl-sn-glycero-3-phosphocholine, 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine, and 1, 2-dioleoyl-sn-glycero-3-phosphatidylcholine) and the increased oxylipin production (12-HETE and 9R, 10S-EpOME). The minor oxidative pathways (omega-oxidation and peroxisomal beta-oxidation) were likely to be induced in HS-treated lenok.

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Data availability

The data used to support the findings of this study are available from the corresponding author upon request.

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Funding

The work was fund support by Beijing technical industry system project (pxm2021 179303 000022), Qinghai Science and Technology Department Project (No. 2018-ZJ-703), and National Natural Science Foundation of China (No. 31760763).

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Authors and Affiliations

  1. Beijing Key Laboratory of Fishery Biotechnology, Fisheries Science Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, People’s Republic of China

    Yan Chen, Shaogang Xu & Xiaofei Yang

  2. College of Eco-Environmental Engineering, Qinghai University, Xining, 810016, People’s Republic of China

    Yang Liu

  3. China Rural Technology Development Center, No.54 Sanlihe Road, Xicheng District, Beijing, 100045, People’s Republic of China

    Yucen Bai

  4. Aquatic Products Quality and Standards Research Center, Chinese Academy of Fishery Sciences, Beijing, 100141, People’s Republic of China

    Bo Cheng

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  1. Yan Chen
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Yan Chen was responsible for experimental design and manuscript writing. Yang Liu took part in sampling and funding acquisition. Yucen Bai supervised the research project and supervised the writing of the manuscript. Shaogang Xu was responsible for preliminary investigation and funding acquisition. Xiaofei Yang took part in experimental procedures. Bo Cheng was responsible for supervising the research project.

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Correspondence to Yucen Bai or Bo Cheng.

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This study was approved by the Institutional Animal Care and Use Committee of Beijing Fisheries Research Institute, Beijing Academy of Agriculture and Forestry Sciences. All experimental procedures were in compliance with the guidelines of Yanqing site of the Beijing Academy of Agriculture and Forestry Sciences, Institute of Fisheries Research, Beijing, China.

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Chen, Y., Liu, Y., Bai, Y. et al. Intestinal metabolomics of juvenile lenok (Brachymystax lenok) in response to heat stress. Fish Physiol Biochem 48, 1389–1400 (2022). https://doi.org/10.1007/s10695-022-01128-7

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