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Novel insights for PI3KC3 in mediating lipid accumulation in yellow catfish Pelteobagrus fulvidraco

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Abstract

In this study, the transcriptional regulation of PI3KC3 by three transcription factors (PPARγ, PPARα, and STAT3) and the potential role of PI3KC3 in mediating lipid accumulation were determined in yellow catfish Pelteobagrus fulvidraco. The 5’-deletion assay, overexpression assay, site-mutation assay, and electrophoretic mobility shift assay suggested that PPARα, PPARγ, and STAT3 negatively regulated the promoter activity of pi3kc3. Moreover, the transcriptional inactivation of pi3kc3 was directly mediated by PPARα and PPARγ under fatty acid (FA) treatment. Using primary hepatocytes from yellow catfish, FA incubation significantly increased triacylglyceride (TG) content, non-esterified fatty acid (NEFA) content, and lipid drops (LDs) content, the mRNA level of pparα, pparγ, stat3, and dnmt3b, the protein level of PPARα, PPARγ, and STAT3, and the methylation level of pi3kc3, but significantly reduced the mRNA and protein level of PI3KC3. Our findings offer new insights into the mechanisms for transcriptional regulation of PI3KC3 and for PI3KC3-mediated lipid accumulation in fish.

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Abbreviations

FA:

Fatty acid

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

HPRT:

Hypoxanthineguanine phosphoribosyltransferase

LDs:

Lipid drops

NEFA:

Non-esterified fatty acid

Mut:

Mutagenesis

NP:

Nuclear protein

PPAR:

Peroxisome proliferator-activated receptor

OE:

Overexpression

PI3K:

Phosphatidylinositol-3 kinase

RPL7:

Ribosomal protein L7

STAT3:

Signal transducers and activators of transcription proteins 3

TG:

Triacylglyceride

TUBA:

Tubulin alpha chain

UBCE:

Ubiquitin-conjugating enzyme

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 31902381).

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Authors and Affiliations

  1. School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, 430070, China

    Mei-Qin Zhuo, Jun Chen, Mei-Li Wu & Wen-Biao Wang

Authors
  1. Mei-Qin Zhuo
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  2. Jun Chen
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  3. Mei-Li Wu
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  4. Wen-Biao Wang
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Contributions

MQ Zhuo designed the experiments; MQ Zhuo and J Chen performed the experiments, and analyzed the samples with the help of ML Wu and WB Wang; J Chen and ML Wu analyzed the data; and J Chen and MQ Zhuo wrote the manuscript. MQ Zhuo revised the manuscript. All the authors approved the manuscript.

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Correspondence to Mei-Qin Zhuo.

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All these animal experiments followed the guideline of the Animal Experimentation Ethics Committee of Wuhan Polytechnic University (WHPU) and were approved by Ethics Committee of WHPU.

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Zhuo, MQ., Chen, J., Wu, ML. et al. Novel insights for PI3KC3 in mediating lipid accumulation in yellow catfish Pelteobagrus fulvidraco. Fish Physiol Biochem 48, 571–583 (2022). https://doi.org/10.1007/s10695-022-01071-7

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