Abstract
In 1996, kiss was reported to regulate feeding in mammals, but studies are limited in fish. Our study aimed to explore the possible role of kiss in the regulation of feeding in Siberian sturgeon (Acipenser baerii). kiss1 and kiss2 were cloned, and the expression patterns were analyzed in Siberian sturgeon. The complete coding regions of kiss1 and kiss2 genes were 393 and 471 bp. Both kiss1 and kiss2 showed the highest expression level in the hypothalamus. During the periprandial and fasting experiments, the expression of kiss1 and kiss2 highly significantly increased in the hypothalamus after feeding (P < 0.01). Compared with the feeding group, in hypothalamus, kiss1 expression in the fasting group highly significantly decreased (P < 0.01). In contrast, kiss2 expression had no significant difference on days 1 and 7 (P > 0.05) but highly significantly increased on day 14 (P < 0.01). Subsequently, the feeding function was verified by intraperitoneal (i.p.) injection of Kp1(10) and Kp1(10) into fish. The results showed that i.p. injection of 1 µg/g BW Kp1(10) or 0.01 µg/g BW Kp2(10) could significantly reduce 0–1 h food intake (P < 0.05) and affected the expression levels of apelin, ghrelin, leptin, nmu, etc. in the hypothalamus. These results suggested that kiss1 plays an anorexic role in both short- and long-term feeding regulation, while kiss2 plays a short-term anorexic and long-term orexigenic role. This study described kiss as a novel regulator of appetite in fish and laid the groundwork for further studies focused on physiological function.
Highlights
• The kiss1 and kiss2 of Siberian sturgeon were cloned.
• The expression levels of kiss1 and kiss2 mRNA were the highest in the hypothalamus.
• Postprandial hypothalamic kiss1 and kiss2 expression levels increased in the periprandial experiment.
• In the fasting test, the expression of hypothalamic kiss1 decreased after fasting, while the expression of kiss2 increased after fasting on the 14th day.
• Siberian sturgeon food intake was reduced, and appetite factors expression levels in the hypothalamus were altered after intraperitoneal injection of Kp1(10) and Kp2(10).
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Funding
This work was supported by the Opening Fund of Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River (no. NJTCCJSYSYS03) and the Sichuan Science and Technology Program grants (no. 2019YJ0438).
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SX and MW contributed equally to this work; they analyzed the data and wrote the paper. ZL, DC, JW, and HC conceived and designed the experiments; YL, SZ, YL, and NT performed the experiments; ZL contributed reagents, materials, and analysis tools. The draft manuscript was revised by ZL, NT, and XZ.
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Shaoqi Xu and Mei Wang paid a similar a mount of work and carried out experiments and thesis writing
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Xu, S., Wang, M., Li, Y. et al. Cloning and expression of kiss genes and regulation of feeding in Siberian sturgeon (Acipenser baerii). Fish Physiol Biochem 48, 419–436 (2022). https://doi.org/10.1007/s10695-022-01055-7
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DOI: https://doi.org/10.1007/s10695-022-01055-7