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Development and characterization of a new gill cell line from the striped catfish, Pangasianodon hypophthalmus (Sauvage, 1878)

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Abstract

Cell lines as an in vitro model developed from different target organs of fish find their use in virus susceptibility, cytotoxicity, gene expression studies. The striped catfish, Pangasianodon hypophthalmus, is one of the main species in aquaculture, especially in Southeast Asian countries like Thailand, Indonesia, China, India, Bangladesh, and Vietnam. The present study reports the development of a new permanent cell line from the gills of P. hypophthalmus designated as PHG and its application in toxicological research. Leibovitz’s L-15 cell culture medium supplemented with 15% fetal bovine serum (FBS) was used to maintain cell line PHG. The morphology of the PHG cell line was observed fibroblastic-like. PHG cells grew well at varying temperatures ranging from 24 to 30 °C with an optimum temperature of 28 °C. The PHG cell line was characterized using a sequence of mitochondrial cytochrome C oxidase subunit I, which authenticated the species of origin of the cell line. The cell line was transfected with a pEGFP-C1 plasmid, and the transfection reporter gene was successfully expressed 48 h post-transfection with 9% transfection efficiency. The toxicity assessment of two organophosphate pesticides, chlorpyrifos, and malathion using the PHG cell line revealed that the two organophosphate pesticides were cytotoxic to the cell line at varying concentrations.

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References

  • Abdul NA, Seepoo AM, Gani T, Sugumar V, Selvam S, Allahbagash B, Abdul Kuthoos AN, Palsamy RK, Kishore M P, M. Rajwade J, Azeez SS (2022) Development and characterization of five novel cell lines from snubnose pompano, Trachinotus blochii (Lacepede, 1801), and their application in gene expression and virological studies. Journal of Fish Diseases. 2022 Jan 1

  • Ahmed VI, Babu VS, Chandra V, Nambi KS, Thomas J, Bhonde R, Hameed AS (2009) A new fibroblastic-like cell line from heart muscle of the Indian major carp (Catla catla): development and characterization. Aquaculture 293(3–4):180–186

    Article  CAS  Google Scholar 

  • Almeida JL, Cole KD, Plant AL (2016) Standards for cell line authentication and beyond. PLoS biology. 14(6):e1002476

  • Avella M, Berhaut J, Payan P (1994) Primary culture of gill epithelial cells from the sea bassDicentrarchus Labrax. In Vitro Cellular & Developmental Biology-Animal 30(1):41–49

    Article  Google Scholar 

  • Bairoch A (2018) The cellosaurus, a cell-line knowledge resource. Journal of Biomolecular Techniques: JBT 29(2):25

    Article  PubMed  PubMed Central  Google Scholar 

  • Bejar J, Borrego JJ, Alvarez MC (1997) A continuous cell line from the cultured marine fish gilt-head seabream (Sparus aurata L.). Aquaculture. 150(1–2):143–53

  • Bieberstein U, Braunbeck T (1998) Light and scanning electron microscopic cytopathology of 3, 5-dichlorophenol in the permanent fish cell line RTG-2. Ecotoxicol Environ Saf 41(3):298–306

    Article  CAS  PubMed  Google Scholar 

  • Bols NC, Lee LE (1991) Technology and uses of cell cultures from the tissues and organs of bony fish. Cytotechnology 6(3):163–187

    Article  CAS  PubMed  Google Scholar 

  • Bols NC, Barlian A, Chirino-Trejo M, Caldwell SJ, Goegan P, Lee LE (1994) Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum), gills. J Fish Dis 17(6):601–611

    Article  Google Scholar 

  • Bols NC, Dayeh VR, Lee LE, Schirmer K (2005) Use of fish cell lines in the toxicology and ecotoxicology of fish. Piscine cell lines in environmental toxicology. InBiochemistry and molecular biology of fishes 2005 Jan 1 (Vol. 6, pp. 43–84). Elsevier

  • Borenfreund E, Puerner JA (1985) A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90). J Tissue Cult Methods 9(1):7–9

    Article  Google Scholar 

  • Chatterjee NS, Banerjee K, Utture S, Kamble N, Rao BM, Panda SK, Mathew S (2016) Assessment of polyaromatic hydrocarbons and pesticide residues in domestic and imported pangasius (Pangasianodon hypophthalmus) fish in India. J Sci Food Agric 96(7):2373–2377

    Article  CAS  PubMed  Google Scholar 

  • Chang SF, Ngoh GH, Kueh LF, Qin QW, Chen CL, Lam TJ, Sin YM (2001) Development of a tropical marine fish cell line from Asian seabass (Lates calcarifer) for virus isolation. Aquaculture 192(2–4):133–145

    Article  Google Scholar 

  • Chi SC, Hu WW, Lo BJ (1999) Establishment and characterization of a continuous cell line (GF-1) derived from grouper, Epinephelus coioides (Hamilton): a cell line susceptible to grouper nervous necrosis virus (GNNV). J Fish Dis 22(3):173–182

    Article  Google Scholar 

  • Cooper JK, Sykes G, King S, Cottrill K, Ivanova NV, Hanner R, Ikonomi P (2007) Species identification in cell culture: a two-pronged molecular approach. In Vitro Cellular & Developmental Biology-Animal 43(10):344–351

    Article  CAS  Google Scholar 

  • Dayeh VR, Schirmer K, Bols NC (2002) Applying whole-water samples directly to fish cell cultures in order to evaluate the toxicity of industrial effluent. Water Res 36(15):3727–3738

    Article  CAS  PubMed  Google Scholar 

  • Dorantes-Aranda JJ, Waite TD, Godrant A, Rose AL, Tovar CD, Woods GM, Hallegraeff GM (2011) Novel application of a fish gill cell line assay to assess ichthyotoxicity of harmful marine microalgae. Harmful Algae 10(4):366–373

    Article  CAS  Google Scholar 

  • Dubey A, Goswami M, Yadav K, Chaudhary D (2015a) Oxidative stress and nano-toxicity induced by TiO2 and ZnO on WAG cell line. PloS one. 10(5)

  • Dubey A, Goswami M, Yadav K, Mishra A, Kumar A (2015b) Establishment of a novel muscle cell line from Wallago attu for in vitro study of pesticide toxicity. Gene, Cell and Tissue

  • Fischer M, Belanger SE, Berckmans P, Bernhard MJ, Bláha L, Coman Schmid DE, Dyer SD, Haupt T, Hermens JL, Hultman MT, Laue H. Repeatability and reproducibility of the RTgill-W1 cell line assay for predicting fish acute toxicity. Toxicological Sciences. 2019 Jun 1;169(2):353–64.Goswami M, Lakra WS, Yadav K, Jena JK. Development of an ES-like cell culture system (RESC) from rohu, Labeo rohita (Ham.). Fish physiology and biochemistry. 2012 Dec 1;38(6):1775–83.

  • Franken NA, Rodermond HM, Stap J, Haveman J, Van Bree C (2006) Clonogenic assay of cells in vitro. Nat Protoc 1(5):2315–2319

    Article  CAS  PubMed  Google Scholar 

  • Freshney RI (2015) Culture of animal cells: a manual of basic technique and specialized applications. John Wiley & Sons

  • Gjessing MC, Aamelfot M, Batts WN, Benestad SL, Dale OB, Thoen E, Weli SC, Winton JR (2018) Development and characterization of two cell lines from gills of Atlantic salmon. Plos one. 13(2):e0191792

  • Goswami M, Yadav K, Dubey A, Sharma BS, Konwar R, Kumar R, Nagpure NS, Lakra WS (2014a) In vitro cytotoxicity assessment of two heavy metal salts in a fish cell line (RF). Drug Chem Toxicol 37(1):48–54

    Article  CAS  PubMed  Google Scholar 

  • Goswami M, Sharma BS, Yadav K, Bahuguna SN, Lakra WS (2014b) Establishment and characterization of a piscean PCF cell line for toxicity and gene expression studies as in vitro model. Tissue Cell 46(3):206–212

    Article  CAS  PubMed  Google Scholar 

  • Goswami M, Dubey A, Yadav K, S Sharma B, S Lakra W (2015) Identification of fish cell lines using 2-D electrophoresis based protein expression signatures. Current Proteomics. 12(4):245–52

  • Hebert PD, Cywinska A, Ball SL, Dewaard JR (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences 270(1512):313–21

  • Hendijani F (2017) Explant culture: An advantageous method for isolation of mesenchymal stem cells from human tissues. Cell proliferation. 50(2):e12334

  • Hightower LE, Renfro JL (1988) Recent applications of fish cell culture to biomedical research. J Exp Zool 248(3):290–302

    Article  CAS  PubMed  Google Scholar 

  • Hollert H, Duerr M, Erdinger L, Braunbeck T (2000) Cytotoxicity of settling particulate matter and sediments of the Neckar River (Germany) during a winter flood. Environmental Toxicology and Chemistry: an International Journal 19(3):528–534

    Article  CAS  Google Scholar 

  • Huang Y, Huang L, Zhang Y, Zheng Q, Xu L, Qin Q, Huang X (2021) Development of a sea perch (Lateolabrax japonicus) fry cell line and its application to the study of fish virus-host interactions. Aquaculture. 25;533:736127

  • Jin Y, Liu Z, Peng T, Fu Z (2015) The toxicity of chlorpyrifos on the early life stage of zebrafish: a survey on the endpoints at development, locomotor behavior, oxidative stress and immunotoxicity. Fish Shellfish Immunol 43(2):405–414

    Article  CAS  PubMed  Google Scholar 

  • Jin YL, Chen LM, Le Y, Li YL, Hong YH, Jia KT, Yi MS (2017) Establishment of a cell line with high transfection efficiency from zebrafish Danio rerio embryos and its susceptibility to fish viruses. J Fish Biol 91(4):1018–1031

    Article  CAS  PubMed  Google Scholar 

  • Katial RK, Sachanandani D, Pinney C, Lieberman MM (1998) Cytokine production in cell culture by peripheral blood mononuclear cells from immunocompetent hosts. Clinical Diagnostic Laboratory Immunology 5(1):78–81

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kulkarni AR, Soppimath KS, Dave AM, Mehta MH, Aminabhavi TM (2000) Solubility study of hazardous pesticide (chlorpyrifos) by gas chromatography. J Hazard Mater 80(1–3):9–13

    Article  CAS  PubMed  Google Scholar 

  • Kumar P, Nagarajan A, Uchil PD (2018) Analysis of cell viability by the MTT assay. Cold spring harbor protocols 2018(6):pdb-rot095505

  • Kumar R, Ravi C, Das S, Dharmaratnam A, Basheer VS, Swaminathan TR (2019) Establishment and characterization of a caudal fin-derived cell line, AOF, from the Oscar. Astronotus Ocellatus Fish Physiology and Biochemistry 45(1):123–131

    Article  CAS  PubMed  Google Scholar 

  • Lakra WS, Sivakumar N, Goswami M, Bhonde RR (2006 Jan) Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch). Aquac Res 37(1):18–24

    Article  Google Scholar 

  • Lakra WS, Singh AK (2010) Risk analysis and sustainability of Pangasianodon hypophthalmus culture in India. Aquaculture Asia 15(1):34–37

    Google Scholar 

  • Lakra WS, Goswami M (2011a) Development and characterization of a continuous cell line PSCF from Puntius sophore. J Fish Biol 78(4):987–1001

    Article  CAS  PubMed  Google Scholar 

  • Lakra WS, Swaminathan TR, Joy KP (2011b) Development, characterization, conservation and storage of fish cell lines: a review. Fish Physiol Biochem 37(1):1–20

    Article  CAS  PubMed  Google Scholar 

  • Lee LE, Dayeh VR, Schirmer K, Bols NC (2009) Applications and potential uses of fish gill cell lines: examples with RTgill-W1. In Vitro Cellular & Developmental Biology-Animal 45(3–4):127–134

    Article  CAS  Google Scholar 

  • Lee JH, Lee ST, Nam YK, Gong SP (2019) Gene delivery into Siberian sturgeon cell lines by commercial transfection reagents. In Vitro Cellular & Developmental Biology-Animal 55(2):76–81

    Article  CAS  Google Scholar 

  • Li Y, Ma Y, Hao L, Ma J, Liang Z, Liu Z, Ke H, Li Y (2021) Characterization of a novel brain cell line from Jian carp (Cyprinus carpio var. Jian). Fish Physiology and Biochemistry. 47(2):439–49

  • Liu Y, Wei C, Liu Z, Cao Z, Sun Y, Zhou Y, Wang S, Guo W (2021) Establishment of a new fish cell line from the brain of humpback grouper (Cromileptes altivelis) and its application in toxicology and bacterial susceptibility. Fish Physiol Biochem 47(5):1645–1658

    Article  CAS  PubMed  Google Scholar 

  • Majeed SA, Nambi KS, Taju G, Raj NS, Madan N, Hameed AS (2013) Establishment and characterization of permanent cell line from gill tissue of Labeo rohita (Hamilton) and its application in gene expression and toxicology. Cell Biol Toxicol 29(1):59–73

    Article  PubMed  CAS  Google Scholar 

  • Majeed SA, Nambi KS, Taju G, Babu VS, Farook MA, Hameed AS (2014) Development and characterization of a new gill cell line from air breathing fish Channa striatus (Bloch 1793) and its application in toxicology: direct comparison to the acute fish toxicity. Chemosphere 1(96):89–98

    Article  CAS  Google Scholar 

  • Markova E, Vasilyev S, Belyaev I (2015) 53BP1 foci as a marker of tumor cell radiosensitivity. Neoplasma 62(5):770–776

    Article  CAS  PubMed  Google Scholar 

  • Meena LL, Goswami M, Chaudhari A, Nagpure NS, Gireesh-Babu P, Dubey A, Das DK (2020) Development and characterization of a new DRCF cell line from Indian wild strain zebrafish Danio rerio (Hamilton 1822). Fish Physiol Biochem 46(4):1337–1347

    Article  CAS  PubMed  Google Scholar 

  • Nanda PK, Swain P, Nayak SK, Behera T, Dhama K (2014) Comparative study on enzymatic and explant method in establishing primary culture from different cultivable cells of Indian major carp Cirrhinus mrigala. Asian J Anim Vet Adv 9(5):281–291

    Article  Google Scholar 

  • Phan LT, Bui TM, Nguyen TT, Gooley GJ, Ingram BA, Nguyen HV, Nguyen PT, De Silva SS (2009) Current status of farming practices of striped catfish, Pangasianodon hypophthalmus in the Mekong Delta. Vietnam Aquaculture 296(3–4):227–236

    Article  Google Scholar 

  • Perry GM, McDonald GJ, Ferguson MM, Ganassin RC, Bols NC (2001) Characterization of rainbow trout cell lines using microsatellite DNA profiling. Cytotechnology 37(3):143–151

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Pomp J, Wike JL, Ouwerkerk IJ, Hoogstraten C, Davelaar J, Schrier PI, Leer JW, Thames HD, Brock WA (1996) Cell density dependent plating efficiency affects outcome and interpretation of colony forming assays. Radiother Oncol 40(2):121–125

    Article  CAS  PubMed  Google Scholar 

  • Rachlin JW, Perlmutter A (1968) Fish cells in culture for study of aquatic toxicants. Water Res 2(6):409–414

    Article  Google Scholar 

  • Rainboth WJ (1996) FAO species identification field guide for fishery purpose. Fish of the Cambodian Mekong, Rome, p 265

    Google Scholar 

  • Roberts TR, Vidthayanon C (1991) Systematic revision of the Asian catfish family Pangasiidae, with biological observations and descriptions of three new species. Proc Acad Natl Sci Phila 1:97–143

    Google Scholar 

  • Rocha A, Ruiz S, Coll JM (2004) Improvement of transfection efficiency of epithelioma papulosum cyprini carp cells by modification of cell cycle and use of an optimal promoter. Mar Biotechnol 6(5):401–410

    Article  CAS  Google Scholar 

  • Romøren K, Thu BJ, Bols NC, Evensen Ø (2004) Transfection efficiency and cytotoxicity of cationic liposomes in salmonid cell lines of hepatocyte and macrophage origin. Biochimica et Biophysica Acta (BBA)-Biomembranes. 1663(1–2):127–34

  • Ruiz-Palacios M, Almeida M, Martins MA, Oliveira M, Esteban MÁ, Cuesta A (2020) Establishment of a brain cell line (FuB-1) from mummichog (Fundulus heteroclitus) and its application to fish virology, immunity and nanoplastics toxicology. Science of The Total Environment 708:134821

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold spring harbor laboratory press

  • Sandbichler AM, Aschberger T, Pelster B (2013) A method to evaluate the efficiency of transfection reagents in an adherent zebrafish cell line. BioResearch Open Access 2(1):20–27

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sathiyanarayanan A, Vijay Sundar Deva G, Nirmal T, Arul Murugan M, Safna P, Kesavan S (2019) Int. J. Chem. Stud 7(4): 1367-1370

  • Schiøtz BL, Rosado EG, Baekkevold ES, Lukacs M, Mjaaland S, Sindre H, Grimholt U, Gjøen T (2011) Enhanced transfection of cell lines from Atlantic salmon through nucleofection and antibiotic selection. BMC Res Notes 4(1):1–7

    Article  Google Scholar 

  • Schirmer K (2006) Proposal to improve vertebrate cell cultures to establish them as substitutes for the regulatory testing of chemicals and effluents using fish. Toxicology 224(3):163–183

    Article  CAS  PubMed  Google Scholar 

  • Segner H. Fish cell lines as a tool in aquatic toxicology. Fish ecotoxicology. 1998:1–38.

  • Segner H (2004) Cytotoxicity assays with fish cells as an alternative to the acute lethality test with fish. Altern Lab Anim 32(4):375–382

    Article  CAS  PubMed  Google Scholar 

  • Singh AK, Lakra WS (2012) Culture of Pangasianodon hypophthalmus into India: impacts and present scenario. Pak J Biol Sci 15(1):19

    Article  CAS  PubMed  Google Scholar 

  • Singh N, Soni P, Kushwaha B, Kumar MS, Srivastava JK, Srivastava S, Mishra AK, Kumar R (2021) Establishment of a testis cell line from Clarias magur: a potential resource for in-vitro applications. The Nucleus 1:1–7

    Google Scholar 

  • Sobhana KS, George KC, Venkat Ravi G, Ittoop G, Paulraj R (2009) Development of a cell culture system from gill explants of the grouper, Epinephelus malabaricus (Bloch and Shneider). Asian Fish Sci 22(2):541–547

    Google Scholar 

  • Soni P, Pradhan PK, Swaminathan TR, Sood N (2018) Development, characterization and application of a new epithelial cell line from caudal fin of Pangasianodon hypophthalmus (Sauvage 1878). Acta Trop 1(182):215–222

    Article  CAS  Google Scholar 

  • Sood N, Chaudhary DK, Pradhan PK, Verma DK, Swaminathan TR, Kushwaha B, Punia P, Jena JK (2015) Establishment and characterization of a continuous cell line from thymus of striped snakehead, Channa striatus (Bloch 1793). In Vitro Cellular & Developmental Biology-Animal 51(8):787–796

    Article  Google Scholar 

  • Sood N, Pradhan PK, Verma DK, Yadav MK, Dev AK, Swaminathan TR, Sood NK (2018) Candidatus Actinochlamydia pangasiae sp. nov.(Chlamydiales, Actinochlamydiaceae), a bacterium associated with epitheliocystis in Pangasianodon hypophthalmus. Journal of fish diseases 41(2):281–90

  • Suryakodi S, Majeed SA, Taju G, Vimal S, Sivakumar S, Ahmed AN, Shah FA, Bhat SA, Sarma D, Begum A, Hameed AS (2021) Development and characterization of novel cell lines from kidney and eye of rainbow trout, Oncorhynchus mykiss for virological studies. Aquaculture 532:736027

  • Taju G, Majeed SA, Nambi KS, Hameed AS (2013) Development and characterization of cell line from the gill tissue of Catla catla (Hamilton, 1822) for toxicological studies. Chemosphere 90(7):2172–2180

    Article  CAS  PubMed  Google Scholar 

  • Taju G, Majeed SA, Nambi KS, Hameed AS (2017) Application of fish cell lines for evaluating the chromium induced cytotoxicity, genotoxicity and oxidative stress. Chemosphere 1(184):1–2

    Article  CAS  Google Scholar 

  • Tanneberger K, Knöbel M, Busser FJ, Sinnige TL, Hermens JL, Schirmer K (2013) Predicting fish acute toxicity using a fish gill cell line-based toxicity assay. Environ Sci Technol 47(2):1110–1119

    Article  CAS  PubMed  Google Scholar 

  • Thangaraj RS, Narendrakumar L, Prasannan Geetha P, Shanmuganathan AR, Dharmaratnam A, Nithianantham SR (2021) Comprehensive update on inventory of finfish cell lines developed during the last decade (2010–2020). Reviews in Aquaculture

  • Thenmozhi C, Vignesh V, Thirumurugan R, Arun S (2011) Impacts of malathion on mortality and biochemical changes of freshwater fish Labeo rohita.

  • Tong SL, Li H, Miao HZ (1997) The establishment and partial characterization of a continuous fish cell line FG-9307 from the gill of flounder Paralichthys olivaceus. Aquaculture 156(3–4):327–333

    Article  Google Scholar 

  • Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PD (2005) DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society b: Biological Sciences 360(1462):1847–1857

    Article  CAS  Google Scholar 

  • Wolf K, Quimby MC (1962) Established eurythermic line of fish cells in vitro. Science 135(3508):1065–1066

    Article  CAS  PubMed  Google Scholar 

  • Xue T, Wang YZ, Pan QH, Wang Q, Yuan JF, Chen TS (2018) Establishment of a cell line from the kidney of black carp and its susceptibility to spring viremia of carp virus. J Fish Dis 41(2):365–374

    Article  CAS  PubMed  Google Scholar 

  • Yan W, Nie P, Lu Y (2011) Establishment, characterization and viral susceptibility of a new cell line derived from goldfish, Carassius auratus (L.), tail fin. Journal of fish diseases. 34(10):757–68

  • Yoon JH, Roh EY, Shin S, Jung NH, Song EY, Chang JY, Kim BJ, Jeon HW (2013) Comparison of explant-derived and enzymatic digestion-derived MSCs and the growth factors from Wharton’s jelly. Biomed Res Int 9:2013

    Google Scholar 

  • Zhou GZ, Gui L, Li ZQ, Yuan XP, Zhang QY (2008) Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus. J Fish Biol 73(8):2058–2067

    Article  CAS  Google Scholar 

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Acknowledgements

The authors wish to express their sincere gratitude to the Director, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra, India, and the Indian Council of Agricultural Research, New Delhi, for providing the facilities and financial support to carry out the research.

Funding

This study was funded by ICAR- Central Institute of Fisheries Education, Mumbai, Maharashtra, India.

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Authors and Affiliations

  1. Fish Genetics and Biotechnology Division, ICAR-Central Institute of Fisheries Education, Panch Marg, Off Yari Road, Versova, Andheri West, Mumbai, 400061, Maharashtra, India

    Arjunan Sathiyanarayanan, Mukunda Goswami, Naresh Nagpure & Gireesh Babu P

  2. Genetic Research Centre, National Institute for Research in Reproductive Health, Parel, Mumbai, 400012, Maharashtra, India

    Dhanjit Kumar Das

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  1. Arjunan Sathiyanarayanan
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Contributions

Arjunan Sathiyanarayanan: Bench work; original draft writing, writing, review, and editing; and methodology.

Naresh Nagpure: Manuscript correction and validation.

Gireesh Babu P: Methodology.

Dhanjit Kumar Das: Methodology.

Mukunda Goswami: Experimental design, conceptualization, and supervision.

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Correspondence to Mukunda Goswami.

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Sathiyanarayanan, A., Goswami, M., Nagpure, N. et al. Development and characterization of a new gill cell line from the striped catfish, Pangasianodon hypophthalmus (Sauvage, 1878). Fish Physiol Biochem 48, 367–380 (2022). https://doi.org/10.1007/s10695-022-01053-9

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