Abstract
Blunt snout bream plays an important role in freshwater aquaculture in China, but the development of its culture industry has been restricted by increasing hypoxia problem. Through the breeding of wild blunt snout bream populations (F0), a hypoxia-tolerant new variety (F6) was obtained. In this study, the new variety was stressed under low oxygen concentration (2.0 mg·L−1) for 4 and 7 days, the morphological structure of the gill tissue showed a striking change, the interlamellar cell mass (ILCM) volume reduced significantly (P < 0.05), and the lamellar respiratory surface area enlarged significantly (P < 0.05), compared to normoxic controls. After 7 days of oxygen recovery, gill remodeling was completely reversed. Additionally, the TUNEL-positive apoptotic fluorescence signals increased in the gills exposed to hypoxia up to 4 and 7 days; the apoptosis rate also increased significantly (P < 0.05). Under 4 and 7 days of hypoxia stress, the expression of anti-apoptotic gene Bcl-2 in the gills downregulated significantly (P < 0.05), with the significantly (P < 0.05) upregulated expression of pro-apoptotic gene Bad. Furthermore, under hypoxia stress, the activity or content of oxidative stress–related enzymes (superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and glutathione (GSH)) in gill tissue increased to varying degrees compared to normoxic controls. These results offer a new perspective into the cellular and molecular mechanism of hypoxia-induced gill remodeling in blunt snout bream and a theoretical basis for its hypoxia adaptation mechanism.
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The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.
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Funding
This work was supported by grants from the National Key Research and Development “Blue Granary Technology Innovation” Key Project (Grant Number: 2020YFD0900400), Capacity Building Plan of Shanghai Local Colleges and Universities (Grant Number: 18050501900), and project funded by the China Postdoctoral Science Foundation (Grant Number: 2019M651473).
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Liang Shuang and Xiao-lei Su carried out the experiment and related analyses. Liang Shuang wrote the manuscript. Shu-ming Zou and Guo-dong Zheng conceived the project and designed scientific objectives. All authors read and approved the final manuscript.
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Shuang, L., Su, Xl., Zheng, Gd. et al. Effects of hypoxia and reoxygenation on gill remodeling, apoptosis, and oxidative stress in hypoxia-tolerant new variety blunt snout bream (Megalobrama amblycephala). Fish Physiol Biochem 48, 263–274 (2022). https://doi.org/10.1007/s10695-022-01047-7
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DOI: https://doi.org/10.1007/s10695-022-01047-7