Abstract
KIF17, which belongs to the kinesin-2 protein family, plays an indispensable role in mammalian spermiogenesis. However, the role of KIF17 in fish spermatid remodeling during spermiogenesis remains poorly understood. Therefore, we aimed to study the role of KIF17 in spermatid remodeling during Larimichthys crocea (L. crocea) spermiogenesis. The kif17 cDNA sequence, 3247 bp in length, was cloned from L. crocea testis, which consisted of a 347-bp 5′-untranslated region (UTR), 413-bp 3′ -UTR, and 2487-bp open reading frame. Bioinformatic analyses revealed that KIF17 obtained from L. crocea (Lc-KIF17) exhibited a high sequence identity compared with those from other teleosts and possessed the structural features of other kinesin-2 proteins. Based on structural similarity, we speculate that the role of Lc-KIF17 may be similar to that of KIF17 in other animals. Lc-kif17 mRNA was diffusely expressed in L. crocea tissues and was highly expressed in the testis, especially at stage IV testicular development. Immunofluorescence analysis revealed that Lc-KIF17 signals colocalized with β-tubulin signals and migrated from the perinuclear cytoplasm to the side of the nucleus where the tail forms during spermiogenesis. These findings revealed that KIF17 may be involved in L. crocea spermiogenesis. In particular, KIF17 may participate in spermatid remodeling by interacting with perinuclear microtubules during L. crocea spermiogenesis. Collectively, this study contributes to an improved understanding of the mechanism underlying L. crocea spermiogenesis and provides a basis for further research on L. crocea reproduction and development.
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This research was funded by the NSFC-Zhejiang Joint Fund for the Integration of Industrialization and Informatization (U1809212), the Scientific and Technical Project of Zhejiang Province (2016C02055-7), the Scientific and Technical Project of Ningbo City (2015C110005), the Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, the K.C. Wong Magna Fund, and Scientific Research Fund (XYL19023) in Ningbo University.
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Conceptualization: JQ Wang, Z Liu, CC Hou, and JQ Zhu; methodology: JQ Wang and Z Liu; formal analysis and investigation: JQ Wang and Z Liu; writing original draft preparation: JQ Wang; writing—review and editing: JQ Wang, XM Gao, DJ Tang, and C Du; funding acquisition: JQ Zhu; resources: B Lou and WL Shen; supervision: JQ Zhu.
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Wang, J., Liu, Z., Gao, X. et al. The potential function of KIF17 in large yellow croaker (Larimichthys crocea) spermatid remodeling: molecular characterization and expression pattern during spermiogenesis. Fish Physiol Biochem 48, 603–616 (2022). https://doi.org/10.1007/s10695-021-01035-3
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DOI: https://doi.org/10.1007/s10695-021-01035-3