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Endoplasmic reticulum stress is involved in lipid accumulation induced by oleic acid in adipocytes of grass carp (Ctenopharyngodon idella): focusing on the transcriptional level

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Abstract

It has been extensively claimed that endoplasmic reticulum stress (ER stress) is related to lipid accumulation in mammals, but little is known in fish. This study aims at elucidating the role of ER stress in mediating lipid accumulation induced by monounsaturated oleic acid (OA) with a focus on the transcriptional level. We treated the adipocytes of grass carp with 200 μM and 400 μM OA, respectively, while the control group was treated with 2% bovine serum albumin (BSA). The results showed that cell viability was significantly improved, while 400 μM OA treatment promoted neutral lipid accumulation along with stimulating ER stress more obviously. Although lipolysis and fatty acid β-oxidation were activated simultaneously, the primary effect of OA seems to be promotion of lipid accumulation. To further explore whether ER stress affects lipid accumulation, 4-phenyl butyric acid (4-PBA), an effective inhibitor of ER stress, was used to pretreat the cells for 4 h. Unsurprisingly, it was found that the mRNA expressions of genes linked with ER stress were decreased. Intracellular triglyceride (TG) content was also decreased, which was in accordance with the mRNA expressions of adipogenic and lipogenic transcription factors as well as their target genes. Collectively, our data shows that ER stress may take part in OA-induced lipid accumulation in adipocytes via activating adipogenesis and lipogenesis. Based on this, strategies for protecting ER could be used to alleviate excessive accumulation of lipid in grass carp adipose tissue.

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Availability of data and material

The datasets and materials used in this study are available from the corresponding author on reasonable request.

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Funding

The study was financially supported by the National Natural Science Foundation of China (NSFC) (31772863, 32072989, and 32002403).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest Agriculture and Forestry University, Yangling, 712100, China

    Chenchen Bian, Jian Sun, Xiaocheng Huang, Shanghong Ji & Hong Ji

Authors
  1. Chenchen Bian
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  2. Jian Sun
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  3. Xiaocheng Huang
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  4. Shanghong Ji
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  5. Hong Ji
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Contributions

Chenchen Bian: contributed to the conception and designed of the study, wrote original draft. Jian Sun: data curation and revised the manuscript. Xiaocheng Huang: investigation and revised the manuscript. Shanghong Ji: methodology and revised the manuscript. Hong Ji: project administration and revised the manuscript. All authors contributed to interpreting the results, and read and approved the final manuscript.

Corresponding author

Correspondence to Hong Ji.

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We promised that all procedures of the experiment were performed in accordance with the Guide for Care and Use of Laboratory Animals and approved by the Northwest A&F University Institutional Animal Care and Use Committee.

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Conflict of interest

This study was supported by College of Animal Science and Technology, Northwest Agriculture and Forestry University. The authors disclose no conflicts of interest. All authors listed have approved to submit to your journal.

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Bian, C., Sun, J., Huang, X. et al. Endoplasmic reticulum stress is involved in lipid accumulation induced by oleic acid in adipocytes of grass carp (Ctenopharyngodon idella): focusing on the transcriptional level. Fish Physiol Biochem 48, 275–284 (2022). https://doi.org/10.1007/s10695-021-01031-7

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  • DOI: https://doi.org/10.1007/s10695-021-01031-7

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