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Role of Zingiber officinale and autochthonous probiotic Bacillus coagulans in feeds of Catla catla (Hamilton, 1822) for growth promotion, immunostimulation, histoprotection, and control of DNA damage

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Abstract

Experiment was conducted to assess the impact of ginger (Zingiber officinale) as a dietary supplement with probiotic bacterium Bacillus coagulans on growth performance, hematological parameters, and non-specific immune parameters in Catla catla. An attempt was also made to investigate their effects on histology and extent of DNA damage against pathogenic bacterium Aeromonas hydrophila in C. catla. Ten dietary treatments were designed in triplicate in which five groups of fingerlings fed on diets containing different incorporation level of dried ginger at 0 g kg−1 (C1; basal diet 1 with duckweed as major protein source), 1 g kg−1 (G1), 5 g kg−1 (G2), 10 g kg−1 (G3), and 15 g kg−1 (G4) and other five groups of fingerlings fed on diets (G5–G9) containing different incorporation level of dried ginger at 0, 1, 5, 10, and 15 g kg−1 along with probiotic bacterium B. coagulans @ 3000 CFU ml−1 for 90 days. Results clearly revealed significantly (P < 0.05) high values of weight gain, growth % gain in body weight, specific growth rate (SGR), RBC and WBC count, phagocytic activity, respiratory burst activity, serum protein, and serum bactericidal activity in group of fingerlings fed on ginger supplemented diets which further improved with the inclusion of probiotic with best results in treatment G8. Histopathological study and COMET assay reflected that Z. officinale and probiotics protect extent of DNA damage and the digestive organs from the detrimental effects of pathogenic bacteria affirming their positive role and harmonious effects of probiotic bacterium with Z. officinale–supplemented diets.

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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

SS is sincerely thankful to Haryana State Council of Science and Technology, Haryana, India, for providing Research Fellowship

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  1. Department of Zoology, Kurukshetra University, Kurukshetra, 136119, Haryana, India

    Anita Bhatnagar & Sonal Saluja

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  1. Anita Bhatnagar
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AB designed the experiment and participated in the preparation of the manuscript; SS carried out the experiment and analyzed the samples/data.

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Bhatnagar, A., Saluja, S. Role of Zingiber officinale and autochthonous probiotic Bacillus coagulans in feeds of Catla catla (Hamilton, 1822) for growth promotion, immunostimulation, histoprotection, and control of DNA damage. Fish Physiol Biochem 47, 2081–2100 (2021). https://doi.org/10.1007/s10695-021-01030-8

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