Abstract
To reveal the impact of dietary fiber (DF) on the bile acid (BA) profiles of fish, yellow catfish (Pelteobagrus fulvidraco) were fed a diet containing 300 g kg−1 dextrin (CON diet, control) or pectin (a type of soluble DF, PEC diet) for 7 days, and then the BA profiles were analyzed by UHPLC-MS/MS. A total of 26 individuals of BAs were detected in the fish body, with 8, 10, 14, and 22 individuals of BAs detected in the liver, serum, bile, and hindgut digesta, respectively. The conjugated BAs (CBAs) of fish were dominated by taurine CBAs (TCBAs). The concentrations of free BAs (FBAs) and the value of FBAs/CBAs in the bile of fish fed the PEC diet were nearly 5 and 7 times higher, respectively than those in fish fed the CON diet. The value of glycine CBAs/TCBAs in the liver, serum and bile of fish fed the PEC diet was significantly lower, and in the hindgut digesta was higher than that of fish fed the CON diet (P < 0.05). These results suggested that dietary pectin greatly changed the BA profiles of Pelteobagrus fulvidraco, attributed to inhibition of reabsorption of BAs. Therefore, attention should be paid to the impact on BA homeostasis when replacing fishmeal with DF-rich plant ingredients in the fish diet.
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References
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This work is supported by the Fishery Science and Technology Projects of Jiangsu Province [Y2018-20], the Major Project of the Natural Science Foundation of the Jiangsu Higher Education Institutions of China [20KJA240001], the Science and Technology Project of Suzhou [SNG2020060] and the Priority Academic Program Development of Jiangsu Higher Education Institutions.
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CXM organized the experiment and was responsible for histological analysis and manuscript writing. RSJ was responsible for biochemical analysis and took part in all experimental procedures. CCF supervised the research project, verified the data, and supervised the writing of the manuscript. NQ was responsible for feed analysis. SY took part in sampling and biochemical analysis. LXY, MYH, MZJ, CHG, and JR were responsible for preliminary investigation and field sample collection for bile acid profiles analysis. WP and YYT took part in experimental designing, data analysis, and writing of the manuscript. All the authors read and approved the final manuscript.
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Cao, X., Ren, S., Cai, C. et al. Dietary pectin caused great changes in bile acid profiles of Pelteobagrus fulvidraco. Fish Physiol Biochem 47, 2015–2025 (2021). https://doi.org/10.1007/s10695-021-01028-2
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DOI: https://doi.org/10.1007/s10695-021-01028-2