Abstract
The deduced amino acid sequences from the complete cDNA coding sequences of three antioxidant enzyme genes (sod1, sod2, and cat) demonstrated that phylogenetically the magur catfish (Clarias magur) is very much close to other bony fishes with complete conservation of active site residues among piscine, amphibian, and mammalian species. The three-dimensional structures of three antioxidant enzyme proteins are very much similar to mammalian counterparts, thereby suggesting the functional similarities of these enzymes. Exposure to ZnO NPs resulted in an oxidative stress as evidenced by an initial sharp rise of intracellular concentrations of hydrogen peroxide (H2O2) and malondialdehyde (MDA) but decreased gradually at later stages. The level of glutathione (GSH) also increased gradually in all the tissues examined after an initial decrease. Biochemical and gene expression analyses indicated that the magur catfish has the ability to defend the ZnO NP-induced oxidative stress by inducing the SOD/CAT enzyme system and also the GSH-related enzymes that are mediated through the activation of various antioxidant-related genes both at the transcriptional and translational levels in various tissues. Furthermore, it appeared that the stimulation of NO, as a consequence of induction nos2 gene, under NP-induced oxidative stress serves as a modulator to induce the SOD/CAT system in various tissues of magur catfish as an antioxidant strategy. Thus, it can be contemplated that the magur catfish possesses a very efficient antioxidant defensive mechanisms to defend against the oxidative stress and also from related cellular damages during exposure to ZnO NPs into their natural environment.
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The authors thank the Head, Department of Zoology, North-Eastern Hill University, Shillong, for allowing us to use the instrumentation facilities in the department in carrying out different experiments and analyses.
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This work was supported by a research project sanctioned to NS by the Science and Engineering Research Board, New Delhi, India (EMR/2016/005263).
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D.K.: methodology, investigation, formal analysis, writing the original draft. B.B: methodology and investigation. A.K.: methodology and investigation. A.S.L: methodology and investigation. R.S.: methodology and investigation. N.S.: conceptualization, validation, formal analysis, investigation, resources, data curation, writing — review and editing — supervision, project administration, and funding acquisition.
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Koner, D., Banerjee, B., Kumari, A. et al. Molecular characterization of superoxide dismutase and catalase genes, and the induction of antioxidant genes under the zinc oxide nanoparticle-induced oxidative stress in air-breathing magur catfish (Clarias magur). Fish Physiol Biochem 47, 1909–1932 (2021). https://doi.org/10.1007/s10695-021-01019-3
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DOI: https://doi.org/10.1007/s10695-021-01019-3