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Identification and expression analysis of cobia (Rachycentron canadum) liver-related miRNAs under hypoxia stress

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Abstract

At present, due to the influence of global warming, seasonal change, diurnal variation, and eutrophication of the water body, hypoxia has become one of the major factors limiting the stable development of cobia (Rachycentron canadum) culture. In this study, the miRNAs involved in hypoxia stress were screened, and the target genes of miRNAs were annotated and analyzed. The results showed that a total of 184 conservative microRNA (miRNA) and 121 newly predicted miRNA were obtained by sequencing the liver of control (C) and hypoxic (dissolved oxygen, DO (2.64 ± 0.25) mg/L; 3 h) (S) groups. The pathways involved in energy metabolism included starch and sucrose metabolism (ko00500), glycosaminoglycan degradation (ko00531), and galactose metabolism (ko00052). The results indicate that the body maintains physiological activities by regulating some important pathways at the transcriptional level under hypoxia stress, such as the conversion of aerobic metabolism and anaerobic metabolism, the reduction of energy consumption, and the promotion of red blood cell proliferation to maintain the homeostasis of the body.

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Upon realistic request, the data supporting this study are accessible from the corresponding author.

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Funding

This work was supported by grants from China Agriculture Research System of MOF and MARA (CARS-47) and Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) (ZJW-2019–06).

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Authors and Affiliations

  1. Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China

    Jian-sheng Huang, Hong-juan Li, Zhi-xiong Guo, Jian-dong Zhang, Wei-zheng Wang, Zhong-liang Wang, Eric Amenyogbe & Gang Chen

  2. Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang, 524025, China

    Jian-sheng Huang, Jian-dong Zhang & Gang Chen

  3. Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, China

    Jian-sheng Huang, Jian-dong Zhang & Gang Chen

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  1. Jian-sheng Huang
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  2. Hong-juan Li
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  3. Zhi-xiong Guo
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  4. Jian-dong Zhang
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  5. Wei-zheng Wang
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Contributions

J.-S.H. and H.-J.L: project administration, data collection, formal analysis, processing and writing—original draft preparation; Z.-S.G.: data collection, data processing; J.-D.Z.: supervision, validation; W.-Z.W.: data collection, data processing; Z.-L.W.: supervision, validation; E.A.: formal analysis, writing—reviewing and editing; and G.C.: conceptualization, methodology, funding acquisition, resources.

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Correspondence to Eric Amenyogbe or Gang Chen.

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In accordance with the regulations for the administration of laboratory animals in Guangdong province, China, this study was conducted compliance with the Guangdong Ocean University Research Council’s guide for the care and use of laboratory animals. Fish were killed with ethyl-3-aminobenzoate methanesulfonate (MS-222; Sigma, USA), for tissue collection.

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Huang, Js., Li, Hj., Guo, Zx. et al. Identification and expression analysis of cobia (Rachycentron canadum) liver-related miRNAs under hypoxia stress. Fish Physiol Biochem 47, 1951–1967 (2021). https://doi.org/10.1007/s10695-021-01017-5

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