Abstract
We investigated blood gas, hematological and biochemical parameters, and gill morphology of Lophiosilurus alexandri juveniles submitted to hypoxia for 48 h, followed by recovery for 48 h. A total of 48 juveniles (360.0 ± 141.6 g) were distributed among eight tanks (120 L) and subjected to hypoxia condition (water with dissolved oxygen at 2.12 ± 0.90 mg L−1) or normoxia (at 5.60 ± 0.31 mg L−1). Blood gas values (pH, PvCO2, PvO2, sO2, HCO3−, stHCO3−, and base excess) in hypoxia were significantly different from normoxia, while for lactate and the electrolytes (K+, Na+, Cl−, and Ca2+) there was no significant change among treatments. The erythrocytes differed significantly between normoxia and hypoxia at 72 h (24 h of recovery), while for hemoglobin and hematocrit there were no significant differences. There was a significant difference in glucose, triglycerides, and cholesterol for both normoxia and hypoxia, while plasma protein remained unchanged. All gill components (epithelial cells, erythrocytes, pillar cells, mucous cells, ionocytes, undifferentiated cells, and blood capillary lumen) differed significantly between normoxia and hypoxia. A reduction in the length of the primary lamella was observed in the hypoxia and recovery treatments, when compared to normoxia. The secondary branchial lamella showed no significant difference for both treatments. Juveniles of Lophiosilurus alexandri adapted well to hypoxia for 48 h, as they were able to adjust most of their physiological variables to survive this stress condition. After 48 h of hypoxia recovery, fish showed parameters similar to animals in normoxia. Thus, the present study shows that the tolerance to hypoxia conditions of L. alexandri, together with other important beneficial characteristics of the species, such as the high meat quality and high commercial value, demonstrates its great potential for production among regional species.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES-Brasil) and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) provided financial support and funding for the author Ronald K. Luz (CNPq—Proc. 308547 / 2018–7).
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Lívia de Assis Porto—conception of design of experiment, conducting the experimental trial and writing of the manuscript.
Rafael Magno Costa Melo—contribution to the writing of the manuscript.
Suzane Lilian Beier—contribution to the writing of the manuscript.
Ronald Kennedy Luz—contribution to conception and design of experiment, statistical analysis and interpretation of data, writing of the manuscript and financial support for the development of the research.
Gisele Cristina Favero – contribution to conception and design of experiment, statistical analysis and interpretation of data and writing of the manuscript.
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de Assis Porto, L., Melo, R.M.C., Beier, S.L. et al. Lophiosilurus alexandri, a sedentary bottom fish, adjusts its physiological parameters to survive in hypoxia condition. Fish Physiol Biochem 47, 1793–1804 (2021). https://doi.org/10.1007/s10695-021-00996-9
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DOI: https://doi.org/10.1007/s10695-021-00996-9