Abstract
Environmental changes in intensive aquaculture commonly lead to hypoxic stress for cultured largemouth bass (Micropterus salmoides). To better to understand the hypoxic stress response mechanisms, the miRNA expression profiles of the livers of largemouth bass exposed for 24 h to three different dissolved oxygen levels (7.0 ± 0.2 mg/L as control, 3.0 ± 0.2 mg/L and 1.2 ± 0.2 mg/L) were compared. In this study, a total of 266 known miRNAs were identified, 84 of which were differentially expressed compared with the control group. Thirteen of the differentially expressed miRNAs (miR-15b-5p, miR-30a-3p, miR-133a-3p, miR-19d-5p, miR-1288-3p, miR456, miR-96-5p, miR-23a-3p, miR-23b-5p, miR-214, miR-24, miR-20a-3p, and miR-2188-5p) were significantly enriched in VEGF signaling pathway, MAPK signaling pathway, and phosphatidylinositol signaling system. These miRNAs were significantly downregulated during stress, especially after a 4-h exposure to hypoxia. In contrast, their target genes (vegfa, pla2g4a, raf1a, pik3c2a, clam2a, inpp1, pi4k2b, mtmr14, ip6k, itpkca, map3k7, and Jun) were significant upregulated after 4 h of hypoxic stress. Moreover, two potential hypoxia-tolerance signal transduction pathways (MAPK signaling pathway and phosphatidylinositol signaling system) were revealed, both of which may play important roles in responding to acute hypoxic stress. We see that miRNAs played an important role in regulating gene expression related to physiological responses to hypoxia.
Potential functional network regulated by miRNAs under hypoixic stress in the liver of largemouth bass (Micropterus salmoides). Blue boxes indicated that the expression of miRNA or target genes were down-regulated. Red boxes indicated that the expression of miRNA or target genes wasere up-regulated.
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Data availability
All data generated and analyzed during this study are included in this published article. The transcriptome sequencing raw data has been uploaded to the NCBI_GEO database, GEO accession number is GSE114714. MiRNA-seq raw data is being uploaded to the NCBI_GEO database, GEO accession number is GSE143641.
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Acknowledgments
We are grateful to MIng Zhou Li, Long Jin, and Ke Ren Long at Sichuan Agriculture University for technical support and software operation, and we gratefully acknowledge the assistance of Hao Wu, Wen Qiang Lian, Qing Xiao, Hong Mei Fu, and Jie Du with fish rearing and tissue sampling.
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This research was supported by the National Natural Science Foundation of China (No.31802267)
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Jun Long Sun, Liu Lan Zhao, and Song Yang conceived and designed the experiments; Jun Long Sun, Kuo He, Dong Mei Zhang, and Lei Liao performed the experiments; Jun Long Sun performed data analysis; Jun Long Sun, Ji Liang, Qiao Liu, and Luo Jie contributed to sample collection; Jun Long Sun wrote the paper; Liu Lan Zhao and Ji Deng Ma assisted with writing and proofreading.
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All experiments were performed according to the Guidelines for the Care and Use of Laboratory Animals in China. All experimental procedures and sample collection were approved by the Institutional Animal Care and Use Committee (IACUC) of the College of Animal Science and Technology of Sichuan Agricultural University, Sichuan, China, under permit no. DKY-B20161701.
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Highlights
1. Acute hypoxia significantly affected miRNA expression patterns.
2. Revealed two new important hypoxia-related signal transduction pathways: the MAPK signaling pathway and the phosphatidylinositol signaling system.
3. Provided several miRNA-mRNA interaction networks under hypoxia.
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Sun, J.L., Zhao, L.L., He, K. et al. MicroRNA regulation in hypoxic environments: differential expression of microRNAs in the liver of largemouth bass (Micropterus salmoides). Fish Physiol Biochem 46, 2227–2242 (2020). https://doi.org/10.1007/s10695-020-00877-7
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DOI: https://doi.org/10.1007/s10695-020-00877-7