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Physiological and metabolic responses in juvenile Colossoma macropomum exposed to hypoxia


This study aimed to evaluate hematological, biochemical, and gasometric parameters of tambaqui juveniles (Colossoma macropomum) exposed to hypoxia and subsequent recovery. Six animals were subjected to normoxia (basal) treatment with dissolved oxygen (DO) 6.27 ± 0.42 mg L−1. Water flow and aeration were reduced for 3 days (hypoxia), during which DO was 0.92 ± 0.37 mg L−1. Water flow and aeration were then reestablished with DO remaining similar to basal. The treatments were as follows: normoxia (basal); 24 h after initiating hypoxia (24H); 72 h after initiating hypoxia (72H); 24 h after reestablishing normoxia (24R); 48 h after reestablishing normoxia (48R); and 96 after reestablishing normoxia (96R). The highest glucose level was recorded at 24H (P < 0.05); the highest lactate level was at 72R; and the highest blood pH was at 24H and 72H (P < 0.05). The highest concentration of PvCO2 was at 24H (P < 0.05), while at 96R it was equivalent to basal (P > 0.05). The variable PvO2 was only higher than basal at 24R (P < 0.05). Juvenile C. macropomum managed to reestablish the main stress indicators (glucose and lactate) at 96R, while the other indicators varied during the study, with homeostatic physiology being reestablished during the recovery period.

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The present research was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES-Brazil - Procad 88887.200588/2018-00), and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG-Brazil). R.K. LUZ received a research grant from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq No. 308547/2018-7).

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Correspondence to Ronald Kennedy Luz.

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The experiment was carried out at Laboratório de Aquacultura of Universidade Federal de Minas Gerais (LAQUA), Brazil, and was approved by the Comitê de Ética em Uso de Animais (Ethics Committee on Animal Use) of UFMG (protocol 296/2019).

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The authors declare that they have no conflict of interest.

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do Carmo Neves, L., Favero, G.C., Beier, S.L. et al. Physiological and metabolic responses in juvenile Colossoma macropomum exposed to hypoxia. Fish Physiol Biochem 46, 2157–2167 (2020).

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  • Hypoxic stress
  • Stress physiology
  • Native fish
  • Tambaqui