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Impact of herbicide pretilachlor on reproductive physiology of walking catfish, Clarias batrachus (Linnaeus)

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Abstract

Herbicide pretilachlor is widely used in paddy fields to control annual weeds. The present study has been carried out in walking catfish, Clarias batrachus, to evaluate the impact of herbicide pretilachlor on reproductive physiology after chronic exposure. Based on the median lethal concentration value (96 h), fish were exposed to three nominal test concentrations of pretilachlor ((SL-I (1/20th LC50), SLII (1/15th LC50), and SL-III (1/10th LC50)) for 30, 45, and 60 days after which plasma sex steroid profile, plasma vitellogenin concentration, and gonadal aromatase activity were analyzed in both sexes. Plasma concentration of testosterone decreases in herbicide-exposed male fish. Significant increase in plasma 17β-estradiol, plasma vitellogenin concentration, and gonadal aromatase activity were observed in herbicide-exposed male fish. All these alterations in reproductive parameters in male fish are dependent on concentration and exposure duration of herbicide. On the other hand, significant decrease in plasma concentration of testosterone was observed in female fish which was also dependent on concentration and exposure duration of herbicide. No significant changes in plasma 17β-estradiol concentrations, plasma vitellogenin concentration, and gonadal aromatase activity were observed in female fish. Above findings clearly suggested that herbicide pretilachlor acts as endocrine disruptor in fish and affects overall reproductive physiology of fish, but its ability to induce reproductive toxicity in male and female differs considerably.

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Acknowledgments

The authors are thankful to Dr. Monika Bhadauria, Head of the department of Zoology for providing lab facilities.

Author contribution statement

S K Verma: conceptualization, supervision, methodology; R. Soni: writing–original draft preparation, investigation, writing–reviewing and editing.

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Authors and Affiliations

  1. Department of Zoology, Guru Ghasidas Vishwavidyalaya (Central University), Bilaspur, Chattisgarh, India

    Rakesh Soni & Sushant Kumar Verma

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  1. Rakesh Soni
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  2. Sushant Kumar Verma
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Correspondence to Sushant Kumar Verma.

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Soni, R., Verma, S.K. Impact of herbicide pretilachlor on reproductive physiology of walking catfish, Clarias batrachus (Linnaeus). Fish Physiol Biochem 46, 2065–2072 (2020). https://doi.org/10.1007/s10695-020-00853-1

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