Abstract
According to the importance of vitamin B6 (pyridoxine) as a water-soluble vitamin on the physiological conditions of aquatic animals, the present study aimed to investigate effects of different concentrations of this vitamin in recycle system culture water on the Nile tilapia (Oreochromis niloticus). Treatments including 0 (control), 10, 20, 30, and 40 mg L−1 vitamin B6 were adjusted in triplicate recirculating systems. Each of the experimental tanks (100 L) was stocked 15 fingerling Nile tilapia during 60-day experimental period. According to the findings, weight gain in treatments of 30 and 40 mg L−1 pyridoxine was significantly higher than the other treatments while blood cortisol hormone in the treatment of 40 mg L−1 was significantly highest among the treatments. In addition, mid-intestine trypsin activity in the treatment of 40 mg L−1 was significantly higher than the other treatments. The histological analysis of the intestine showed that the number of mucus-secreting cells significantly decreased in treatments of 30 and 40 mg L−1. Our findings here suggest that pyridoxine can possibly be absorbed by the Nile tilapia’s body through culture water and it seems 20–30 mg L−1 pyridoxine in the culture water is the optimal concentration for the Nile tilapia juveniles in recycle system culture.
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References
Albrektsen S, Hagve TA, Lie Q (1994) The effect of dietary vitamin B6 on tissue fat contents and lipid composition in livers and gills of Atlantic salmon (Salmo salar). Comp Biochem Physiol A Physiol 109:403–411. https://doi.org/10.1016/0300-9629(94)90144-9
Albrektsen S, Sandnes K, Glette J, Waagbø R (1995) Influence of dietary vitamin B6 on tissue vitamin B6 contents and immunity in Atlantic salmon, Salmo salar L. Aquac Res 26:331–339. https://doi.org/10.1111/j.1365-2109.1995.tb00921.x
Barron MG, Adelman IR (1984) Nucleic acid, protein content, and growth of larval fish sublethally exposed to various toxicants. Can J Fish Aquat Sci 41:141–150. https://doi.org/10.1139/f84-014
Bier M (1955) Lipases. Methods in enzymology I. Academic Press, 1: 627–642
Calderon-Guzman D, Hernandez-Islas JL, Espitia-Vazquez I, Barragan-Mejia G, Hernandez-Garcia E, Santamaria-del Angel D, Juarez-Olguin H (2004) Pyridoxine, regardless of serotonin levels, increases production of 5-hydroxytryptophan in rat brain. Arch Med Res 35:271–274. https://doi.org/10.1016/j.arcmed.2004.03.003
Chou MY, Yang CH, Lu FI, Lin HC, Hwang PP (2002) Modulation of calcium balance in tilapia larvae (Oreochromis mossambicus) acclimated to low-calcium environments. J Comp Physiol B 172:109–114. https://doi.org/10.1007/s00360-001-0231-2
Cortijo M, Llor J, Sanchez-Ruiz JM (1988) Thermodynamic constants for tautomerism, hydration, and ionization of vitamin B6 compounds in water/dioxane. J Biol Chem 263(34):17960–17969
Coyle SD, Mengel GJ, Tidwell JH, Webster CD (2004) Evaluation of growth, feed utilization, and economics of hybrid tilapia, Oreochromis niloticus× Oreochromis aureus, fed diets containing different protein sources in combination with distillers dried grains with solubles. Aquac Res 35:365–370. https://doi.org/10.1111/j.1365-2109.2004.01023.x
Deng SX, Tian LX, Liu FJ, Jin SJ, Liang GY, Yang HJ, Liu YJ (2010) Toxic effects and residue of aflatoxin B1 in tilapia (Oreochromis niloticus× O. aureus) during long-term dietary exposure. Aquaculture 307:233–240. https://doi.org/10.1016/j.aquaculture.2010.07.029
Depeint F, Bruce WR, Shangari N, Mehta R, O’Brien PG (2006) Mitochondrial function and toxicity: the role of B vitamins on the one-carbon transfer pathways. Chem Biol Interact 163:113–132. https://doi.org/10.1016/j.cbi.2006.05.010
Figueiredo-Silva A, Rocha E, Dias J, Silva P, Rema P, Gomes E, Valente LMP (2005) Partial replacement of fish oil by soybean oil on lipid distribution and liver histology in European sea bass (Dicentrarchus labrax) and rainbow trout (Oncorhynchus mykiss) juveniles. Aquac Nutr 11:147–155. https://doi.org/10.1111/j.1365-2095.2004.00337.x
Food and Agriculture Organization of the United Nations (FAO) (2018) Fishery and Aquaculture Statistics, FAO, Rome, Italy
Godfray HCJ, Beddington JR, Crute IR, Haddad L, Lawrence D, Muir JF, Toulmin C (2010) Food security: the challenge of feeding 9 billion people. Science 327:812–818. https://doi.org/10.1126/science.1185383
Hummel BCW (1959) A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Can J Biochem Physiol 37:1393–1399. https://doi.org/10.1139/o59-157
Ink SL, Henderson LM (1984) Vitamin B6 metabolism. Annu Rev Nutr 4:455–470. https://doi.org/10.1146/annurev.nu.04.070184.002323
Johansson S, Lindstedt S, Tiselius HG (1974) Metabolic interconversions of different forms of vitamin B6. J Biol Chem 249:6040–6046
Kannan K, Jain SK (2004) Effect of vitamin B6 on oxygen radicals, mitochondrial membrane potential, and lipid peroxida-tion in H2O2-treated U937 monocytes. Free Radic Biol Med 36:423–428. https://doi.org/10.1016/j.freeradbiomed.2003.09.012
Karmen A, Wroblewski F, Ladue JS (1955) Transaminase activity in human blood. J Clin Invest 34:126–133. https://doi.org/10.1172/JCI103055
Kumar N, Ambasankar K, Krishnani KK, Gupta SK, Minha PS (2016) Dietary pyridoxine promotes growth and cellular metabolic plasticity of Chanos chanos fingerlings exposed to endosulfan induced stress. Aquac Res 48:2074–2087. https://doi.org/10.1111/are.13042
Lepkovsky S (1930) The distribution of serum and plasma proteins in fish. J Biol Chem 85:667–673
Lichstein HC, Gunsalus IC, Umbreit WW (1945) The function of the vitamin B6 group; pyridoxal phosphate (codecarboxylase) in transamination. J Biol Chem 161:311–320
Liti D, Cherop L, Munguti J, Chhorn L (2005) Growth and economic performance of Nile tilapia (Oreochromis niloticus L.) fed on two formulated diets and two locally available feeds in fertilized ponds. Aquac Res 36:746–752. https://doi.org/10.1111/j.1365-2109.2005.01265.x
Liu CH, Chiu CS, Ho PL, Wang SW (2009) Improvement in the growth performance of white shrimp, Litopenaeus vannamei, by a protease-producing probiotic, Bacillus subtilis E20, from natto. J Appl Microbiol 107:1031–1041. https://doi.org/10.1111/j.1365-2672.2009.04284.x
Lumeng L, Li TK (1974) Vitamin B6 metabolism in chronic alcohol abuse: Pyridoxal phosphate levels in plasma and the effects of acetaldehyde on prydoxal phaosphate synthesis and degradation in human erythrocytes. J Clin Invest 53:693–704. https://doi.org/10.1172/JCI107607
Matos P, Fontaı A, Peixoto F, Carrola J, Rocha E (2007) Biochemical and histological hepatic changes of Nile tilapia Oreochromis niloticus exposed to carbaryl. Pestic Biochem Physiol 89:73–80. https://doi.org/10.1016/j.pestbp.2007.03.002
Parry G (1966) Osmotic adaptation in fishes. Biol Rev 41:392–440. https://doi.org/10.1111/j.1469-185X.1966.tb01499.x
Rafiee G, Tavabe KR, Frinsko M, Daniels H (2015) Effects of various sodium adsorption ratio (SAR) mediums on larval performance of the freshwater prawn Macrobrachium rosenbergii (de Man). Aquac Res 46:725–735. https://doi.org/10.1111/are.12219
Refstie S, Landsverk T, Bakke-McKellep AM, Ringø E, Sundby A, Shearer KD, Krogdahl Å (2006) Digestive capacity, intestinal morphology, and microflora of 1-year and 2-year old Atlantic cod (Gadus morhua) fed standard or bio-processed soybean meal. Aquaculture 261:269–284. https://doi.org/10.1016/j.aquaculture.2006.07.011
Ricker WE (1975) Computation and interpretation of biological statistics of fish populations. J Fish Res Board Can 191:1–382 https://ci.nii.ac.jp/naid/10020024399/
Rogers KS, Mohan C (1994) Vitamin B6 metabolism and diabetes. Biochemical Medicine and Metabolic Biology 52:10–17. https://doi.org/10.1006/bmmb.1994.1027
Selhub J, Jacques PF, Wilson PW, Rush D, Rosenberg IH (1993) Vitamin status and intake as primary determinants of homocysteinemia in an elderly population. JAMA 270:2693–2698. https://doi.org/10.1001/jama.1993.03510220049033
Shiau SY, Hsieh HL (1997) Vitamin B6 requirements of tilapia Oreochromis niloticus x O. aureus fed two dietary protein concentrations. Fish Sci 63:1002–1007. https://doi.org/10.2331/fishsci.63.1002
Storvick CA, Benson EM, Edwards MA, Woodring MJ (1964) Chemical and microbiological determination of vitamin B6. Methods Biochem Anal 12:183. https://doi.org/10.1002/9780470110300.ch5
Tavabe KR, Rafiee G, Frinsko M, Daniels H (2013) Effects of different calcium and magnesium concentrations separately and in combination on Macrobrachium rosenbergii (de Man) larviculture. Aquaculture 412:160–166. https://doi.org/10.1016/j.aquaculture.2013.07.023
Teixeira CP, Barros MM, Pezzato LE, Fernandes AC, Albers Koch JF, Padovani CR (2012) Growth performance of Nile tilapia (Oreochromis niloticus), fed diets containing levels of pyridoxine and haematological response under heat stress. Aquac Res 43:1081–1088. https://doi.org/10.1111/j.1365-2109.2011.02911.x
Thiele VF, Brin M (1966) Chromatographic separation and microbiologic assay of vitamin B6 in tissues from normal and vitamin B6-depleted rats. J Nutr 90:347–353. https://doi.org/10.1093/jn/90.4.347
Toepfer EW, Polansky MM (1970) Microbiological assay of vitamin B6 and its components. J Assoc Off Anal Chem 53:546–550
Van den Berg H, Van Schaik F, Finglas PM, de Froidmont-Görtz I (1996) Third EU MAT intercomparison on methods for the determination of vitamins B-1, B-2 and B-6 in food. Food Chem 57:101–108. https://doi.org/10.1016/0308-8146(96)00145-8
Wikman A, Karlsson J, Carlstedt I, Artursson P (1993) A drug absorption model based on the mucus layer producing human intestinal goblet cell line HT29-H. Pharm Res 10:843–852. https://doi.org/10.1023/A:1018905109971
Wotton ID, Freeman H (1982) Microanalysis in medical biochemistry. Churchill, New York, USA
Acknowledgments
The authors wish to thank the Fisheries Department of Natural Resources Faculty of the University of Tehran for providing experimental facilities. Also, we would like to sincerely thank Prof. Rafiee for their technical assistance.
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Current research was funded by the University of Tehran under grant number 26713.
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The trial protocol was approved by the Ethics Committee for the Animal Research, University of Tehran; none of the fish suffered starvation, trauma, or electrical shock and all the fish were completely anesthetized before tissue sampling.
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Javanmardi, S., Rezaei Tavabe, K., Rosentrater, K.A. et al. Effects of different levels of vitamin B6 in tank water on the Nile tilapia (Oreochromis niloticus): growth performance, blood biochemical parameters, intestine and liver histology, and intestinal enzyme activity. Fish Physiol Biochem 46, 1909–1920 (2020). https://doi.org/10.1007/s10695-020-00840-6
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DOI: https://doi.org/10.1007/s10695-020-00840-6