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The effect of teprenone on the intestinal morphology and microbial community of Chinese sea bass (Lateolabrax maculatus) under intermittent hypoxic stress

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Abstract

Hypoxia stress may affect the fish intestine and thereby threaten the growth and survival of the fish. Teprenone is a clinically effective agent in protecting gastrointestinal mucosa. This study aims to assess the effect of teprenone in the intestine of Chinese sea bass Lateolabrax maculatus under intermittent hypoxic stress. L. maculatus juveniles were either raised under intermittent hypoxic condition or normal condition (NC). Part of the hypoxic-intervened fish were treated with teprenone at different concentrations (HTs), and the rest were regarded as hypoxic control (HC). Histological analysis was performed on the epithelial tissue of the fish intestine. High-throughput sequencing technology was used to analyze the diversity and composition of the microbial community in L. maculatus intestine. Reduced villi length and goblet cell, exfoliated enterocyte, and improper arrangement of villi were observed in HC compared with NC and HTs. Proteobacteria, Firmicutes, and Bacteroidetes represented the most abundant phyla in each sample. Significantly higher microbial diversity was detected in HC compared with NC (P < 0.05). At the phylum level, HC presented significantly decreased relative abundance of Proteobacteria, and significantly increased relative abundance of Bacteroidetes, Chloroflex, and Cyanobacteria compared with NC (P < 0.05). At the class level, HC showed significantly reduced relative abundance of Alphaproteobacteria and Bacilli, and significantly increased relative abundance of Clostridia, Gammaproteobacteria, and Bacteroides (P < 0.05). Teprenone protects the intestine from epithelial damages and maintains the microbial harmony in L. maculatus under intermittent hypoxic stress.

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  • 10 October 2020

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Authors and Affiliations

  1. Fisheries Engineering Institute, Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 231 West Xingang Road, Haizhu District, Guangzhou, 510300, China

    Hong-biao Dong, Ya-fei Duan, Hua Li, Qing-song Liu & Jia-song Zhang

  2. State Key Laboratory of Marine Environmental Science, College of Marine and Earth Sciences, Xiamen University, Xiamen, China

    Yong-xu Sun

  3. Zhuhai Modern Agricultural Development Center, Zhuhai, Guangdong, China

    Yong Li

  4. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China

    Wen-hao Wang

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  1. Hong-biao Dong
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  2. Yong-xu Sun
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  3. Ya-fei Duan
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  4. Hua Li
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  5. Yong Li
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Correspondence to Jia-song Zhang.

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Fig. S1

Schematic diagram of the modified tank for the detection and management of dissolved oxygen. 1: cultivation tank; 2: nitrogen device; 3: air device; 4: central control unit; 5: input device; 6: warning device; 7: monitoring device; 8: block device; 9: dissolved oxygen probe; 10: water-driving device; 11 and 12: nano-aerator pipe; 13: drain hole. (JPG 28 kb)

Fig. S2

The diagram of experimental setup. NC: fish neither exposed to hypoxic stress nor treated by teprenone; HC: hypoxia-induced fish without teprenone treatment; HT200: hypoxia-exposed fish treated by teprenone at 200 mg/kg; HT400: hypoxia-exposed fish treated by teprenone at 400 mg/kg; HT800: hypoxia-exposed fish treated by teprenone at 800 mg/kg. (JPG 168 kb)

Fig. S3

Rarefaction curve of the samples. NC: fish neither exposed to hypoxic stress nor treated by teprenone; HC: hypoxia-induced fish without teprenone treatment; HT200: hypoxia-exposed fish treated by teprenone at 200 mg/kg; HT400: hypoxia-exposed fish treated by teprenone at 400 mg/kg; HT800: hypoxia-exposed fish treated by teprenone at 800 mg/kg. (JPG 201 kb)

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Dong, Hb., Sun, Yx., Duan, Yf. et al. The effect of teprenone on the intestinal morphology and microbial community of Chinese sea bass (Lateolabrax maculatus) under intermittent hypoxic stress. Fish Physiol Biochem 46, 1873–1882 (2020). https://doi.org/10.1007/s10695-020-00838-0

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