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Molecular identification of grass carp igfbp2 and the effect of glucose, insulin, and glucagon on igfbp2 mRNA expression

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Abstract

The GH (growth hormone)/IGFs (insulin-like growth factors) system has an important function in the regulation of growth. In this system, IGFBPs play a crucial regulatory role in IGF functions. As a member of the IGFBP family, IGFBP2 can bind to IGF and regulate IGF functions to regulate development and growth. In addition, IGFBP2 shows key regulatory functions in cell proliferation and metabolism. In this study, the igfbp2 gene was cloned from grass carp (Ctenopharyngodon idellus) liver. The ORF of grass carp igfbp2 is 834 bp long and encodes 277 amino acids. The tissue distribution results showed that igfbp2 is expressed in multiple tissues in grass carp and has a high expression level in the liver. In the OGTT, igfbp2 expression was significantly decreased in the liver and brain after 6 h of treatment with glucose. In vitro, igfbp2 expression in grass carp’s primary hepatocytes was significantly suppressed by insulin after treatment for 6 and 12 h. Moreover, igfbp2 expression was markedly increased in a dose-dependent manner with glucagon incubation in grass carp’s primary hepatocytes. To the best of our knowledge, this is the first report about Igfbp2 in grass carp. These results will provide a basis for the in-depth study of grass carp Igfbp2.

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Abbreviations

GH:

Growth hormone

IGF:

Insulin-like growth factor

IGFBPs:

IGF binding proteins

ORF:

Open reading frame

OGTT:

Oral glucose tolerance test

GHR:

GH receptor

ALS:

Acid-labile subunit

RGD:

Arg-Gly-Asp

RT-PCR:

Reverse transcription PCR

cDNA:

Complementary DNA

PCR:

Polymerase Chain Reaction

PBS:

Phosphate buffer solution

FBS:

Fetal bovine serum

HBD:

Heparin-binding domain

T2DM:

Type 2 diabetes mellitus

C/EBPα:

CCAAT/enhancer binding protein α

PI3K:

Phosphatidylinositol 3-kinase

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Funding

This work was supported by the National Natural Science Foundation of China (31702358, 31672671, 31872581, and 31372540), the NSFC-United Fund Key Support Project of Henan (U1704109), the Key Scientific and Technology Research Project of Henan Province (192102110193, 182102110197, and 182102110284), the Key Scientific Research Projects of Henan Province (20A240005), the Doctoral Fund of Henan Normal University (5101229170134), and the Innovation Scientists and Technicians Troop Construction Projects of Henan Province (CXTD2016043).

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Authors and Affiliations

  1. College of Fisheries, Henan Normal University, Xinxiang, 453007, People’s Republic of China

    Guokun Yang, Wenli Zhao, Chaobin Qin, Liping Yang, Xiaolin Meng, Ronghua Lu, Xiao Yan, Xianglin Cao, Yanmin Zhang & Guoxing Nie

  2. College of Fisheries, Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang, 453007, People’s Republic of China

    Guokun Yang, Wenli Zhao, Chaobin Qin, Liping Yang, Xiaolin Meng, Ronghua Lu, Xiao Yan, Xianglin Cao, Yanmin Zhang & Guoxing Nie

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  1. Guokun Yang
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  2. Wenli Zhao
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  3. Chaobin Qin
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  4. Liping Yang
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  5. Xiaolin Meng
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  9. Yanmin Zhang
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  10. Guoxing Nie
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Correspondence to Guoxing Nie.

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Highlights

igfbp2 was cloned and highly expressed in grass carp liver and brain.

igfbp2 was regulated by OGTT in liver and brain.

igfbp2 was regulated by insulin/glucagon in primary grass carp hepatocytes.

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Yang, G., Zhao, W., Qin, C. et al. Molecular identification of grass carp igfbp2 and the effect of glucose, insulin, and glucagon on igfbp2 mRNA expression. Fish Physiol Biochem 46, 1469–1482 (2020). https://doi.org/10.1007/s10695-020-00804-w

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